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Peste des Petits Ruminants Virus Exhibits Cell-Dependent Interferon Active Response
Peste des petits ruminants (PPR) is an acute and highly pathogenic infectious disease caused by peste des petits ruminants virus (PPRV), which can infect goats and sheep and poses a major threat to the small ruminants industry. The innate immune response plays an important role as a line of defense...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195304/ https://www.ncbi.nlm.nih.gov/pubmed/35711660 http://dx.doi.org/10.3389/fcimb.2022.874936 |
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author | Tang, Jingyu Tang, Aoxing Du, Hanyu Jia, Nannan Zhu, Jie Li, Chuanfeng Meng, Chunchun Liu, Guangqing |
author_facet | Tang, Jingyu Tang, Aoxing Du, Hanyu Jia, Nannan Zhu, Jie Li, Chuanfeng Meng, Chunchun Liu, Guangqing |
author_sort | Tang, Jingyu |
collection | PubMed |
description | Peste des petits ruminants (PPR) is an acute and highly pathogenic infectious disease caused by peste des petits ruminants virus (PPRV), which can infect goats and sheep and poses a major threat to the small ruminants industry. The innate immune response plays an important role as a line of defense against the virus. The effect of PPRV on the active innate immune response has been described in several studies, with different conclusions. We infected three goat-derived cell lines with PPRV and tested their innate immune response. PPRV proliferated in caprine endometrial epithelial cells (EECs), caprine skin fibroblasts cells (GSFs), and goat fibroblast cells (GFs), and all cells expressed interferon (IFN) by poly (I: C) stimulation. PPRV infection stimulated expression of type I and type III IFN on EECs, and expression of the latter was significantly stronger, but IFN was not stimulated in fibroblasts (GSFs and GFs). Our results suggested that the effect of PPRV on IFN was cell-type specific. Nine IFN-stimulated genes (ISGs) were detected in EECs, but only ISG15 and RSAD2 were significantly upregulated. The effects of PPRV on IFN and IFN-induced ISGs were cell-type specific, which advances our understanding of the innate immune response induced by PPRV and creates new possibilities for the control of PPRV infection. |
format | Online Article Text |
id | pubmed-9195304 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91953042022-06-15 Peste des Petits Ruminants Virus Exhibits Cell-Dependent Interferon Active Response Tang, Jingyu Tang, Aoxing Du, Hanyu Jia, Nannan Zhu, Jie Li, Chuanfeng Meng, Chunchun Liu, Guangqing Front Cell Infect Microbiol Cellular and Infection Microbiology Peste des petits ruminants (PPR) is an acute and highly pathogenic infectious disease caused by peste des petits ruminants virus (PPRV), which can infect goats and sheep and poses a major threat to the small ruminants industry. The innate immune response plays an important role as a line of defense against the virus. The effect of PPRV on the active innate immune response has been described in several studies, with different conclusions. We infected three goat-derived cell lines with PPRV and tested their innate immune response. PPRV proliferated in caprine endometrial epithelial cells (EECs), caprine skin fibroblasts cells (GSFs), and goat fibroblast cells (GFs), and all cells expressed interferon (IFN) by poly (I: C) stimulation. PPRV infection stimulated expression of type I and type III IFN on EECs, and expression of the latter was significantly stronger, but IFN was not stimulated in fibroblasts (GSFs and GFs). Our results suggested that the effect of PPRV on IFN was cell-type specific. Nine IFN-stimulated genes (ISGs) were detected in EECs, but only ISG15 and RSAD2 were significantly upregulated. The effects of PPRV on IFN and IFN-induced ISGs were cell-type specific, which advances our understanding of the innate immune response induced by PPRV and creates new possibilities for the control of PPRV infection. Frontiers Media S.A. 2022-05-31 /pmc/articles/PMC9195304/ /pubmed/35711660 http://dx.doi.org/10.3389/fcimb.2022.874936 Text en Copyright © 2022 Tang, Tang, Du, Jia, Zhu, Li, Meng and Liu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Tang, Jingyu Tang, Aoxing Du, Hanyu Jia, Nannan Zhu, Jie Li, Chuanfeng Meng, Chunchun Liu, Guangqing Peste des Petits Ruminants Virus Exhibits Cell-Dependent Interferon Active Response |
title | Peste des Petits Ruminants Virus Exhibits Cell-Dependent Interferon Active Response |
title_full | Peste des Petits Ruminants Virus Exhibits Cell-Dependent Interferon Active Response |
title_fullStr | Peste des Petits Ruminants Virus Exhibits Cell-Dependent Interferon Active Response |
title_full_unstemmed | Peste des Petits Ruminants Virus Exhibits Cell-Dependent Interferon Active Response |
title_short | Peste des Petits Ruminants Virus Exhibits Cell-Dependent Interferon Active Response |
title_sort | peste des petits ruminants virus exhibits cell-dependent interferon active response |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195304/ https://www.ncbi.nlm.nih.gov/pubmed/35711660 http://dx.doi.org/10.3389/fcimb.2022.874936 |
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