Re-track: Software to analyze the retraction and protrusion velocities of neurites, filopodia and other structures

We have developed new software, Re-track, that will quantify the rates of retraction and protrusion of structures emanating from the central core of a cell, such as neurites or filopodia. Re-Track, uses time-lapse images of cells in TIFF format and calculates the velocity of retraction or protrusion...

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Detalles Bibliográficos
Autores principales: Mousavi, Sayed Iman, Pearce, Katherine M., Scarlata, Suzanne, Tüzel, Erkan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195366/
https://www.ncbi.nlm.nih.gov/pubmed/32081618
http://dx.doi.org/10.1016/j.ab.2020.113626
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author Mousavi, Sayed Iman
Pearce, Katherine M.
Scarlata, Suzanne
Tüzel, Erkan
author_facet Mousavi, Sayed Iman
Pearce, Katherine M.
Scarlata, Suzanne
Tüzel, Erkan
author_sort Mousavi, Sayed Iman
collection PubMed
description We have developed new software, Re-track, that will quantify the rates of retraction and protrusion of structures emanating from the central core of a cell, such as neurites or filopodia. Re-Track, uses time-lapse images of cells in TIFF format and calculates the velocity of retraction or protrusion of a selected structure. The software uses a flexible moving boundary and has the ability to correct this boundary throughout analysis. Re-Track is fast, platform independent, and user friendly, and it can be used to follow biological events such as changes in neuronal connections, tip-growing cells such as moss, adaptive migration of cells, and similar behavior in non-biological systems.
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spelling pubmed-91953662022-06-14 Re-track: Software to analyze the retraction and protrusion velocities of neurites, filopodia and other structures Mousavi, Sayed Iman Pearce, Katherine M. Scarlata, Suzanne Tüzel, Erkan Anal Biochem Article We have developed new software, Re-track, that will quantify the rates of retraction and protrusion of structures emanating from the central core of a cell, such as neurites or filopodia. Re-Track, uses time-lapse images of cells in TIFF format and calculates the velocity of retraction or protrusion of a selected structure. The software uses a flexible moving boundary and has the ability to correct this boundary throughout analysis. Re-Track is fast, platform independent, and user friendly, and it can be used to follow biological events such as changes in neuronal connections, tip-growing cells such as moss, adaptive migration of cells, and similar behavior in non-biological systems. 2020-05-01 2020-02-17 /pmc/articles/PMC9195366/ /pubmed/32081618 http://dx.doi.org/10.1016/j.ab.2020.113626 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Mousavi, Sayed Iman
Pearce, Katherine M.
Scarlata, Suzanne
Tüzel, Erkan
Re-track: Software to analyze the retraction and protrusion velocities of neurites, filopodia and other structures
title Re-track: Software to analyze the retraction and protrusion velocities of neurites, filopodia and other structures
title_full Re-track: Software to analyze the retraction and protrusion velocities of neurites, filopodia and other structures
title_fullStr Re-track: Software to analyze the retraction and protrusion velocities of neurites, filopodia and other structures
title_full_unstemmed Re-track: Software to analyze the retraction and protrusion velocities of neurites, filopodia and other structures
title_short Re-track: Software to analyze the retraction and protrusion velocities of neurites, filopodia and other structures
title_sort re-track: software to analyze the retraction and protrusion velocities of neurites, filopodia and other structures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195366/
https://www.ncbi.nlm.nih.gov/pubmed/32081618
http://dx.doi.org/10.1016/j.ab.2020.113626
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