Cargando…

CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition

Tumor metastasis and invasion are the main impediments to lung adenocarcinoma successful treatment. Previous studies demonstrate that chemotherapeutic agents can elevate the malignancy of cancer cells other than their therapeutic effects. In this study, the effects of transient low-dose cisplatin tr...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Ruijie, Li, Shengjin, Lan, Jian, Li, Changyi, Du, Xianzhi, Dong, Weijie, Yu, Qian, Wang, Daoxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196332/
https://www.ncbi.nlm.nih.gov/pubmed/35711928
http://dx.doi.org/10.3389/fgene.2022.891665
_version_ 1784727164484059136
author Zhang, Ruijie
Li, Shengjin
Lan, Jian
Li, Changyi
Du, Xianzhi
Dong, Weijie
Yu, Qian
Wang, Daoxin
author_facet Zhang, Ruijie
Li, Shengjin
Lan, Jian
Li, Changyi
Du, Xianzhi
Dong, Weijie
Yu, Qian
Wang, Daoxin
author_sort Zhang, Ruijie
collection PubMed
description Tumor metastasis and invasion are the main impediments to lung adenocarcinoma successful treatment. Previous studies demonstrate that chemotherapeutic agents can elevate the malignancy of cancer cells other than their therapeutic effects. In this study, the effects of transient low-dose cisplatin treatment on the malignant development of lung adenocarcinoma cells (A549) were detected, and the underlying epigenetic mechanisms were investigated. The findings showed that A549 cells exhibited epithelial-mesenchymal transition (EMT)-like phenotype along with malignant progression under the transient low-dose cisplatin treatment. Meanwhile, low-dose cisplatin was found to induce contactin-1 (CNTN-1) upregulation in A549 cells. Subsequently, we found that further overexpressing CNTN-1 in A549 cells obviously activated the EMT process in vitro and in vivo, and caused malignant development of A549 cells in vitro. Taken together, we conclude that low-dose cisplatin can activate the EMT process and resulting malignant progression through upregulating CNTN-1 in A549 cells. The findings provided new evidence that a low concentration of chemotherapeutic agents could facilitate the malignancy of carcinoma cells via activating the EMT process other than their therapeutic effects.
format Online
Article
Text
id pubmed-9196332
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91963322022-06-15 CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition Zhang, Ruijie Li, Shengjin Lan, Jian Li, Changyi Du, Xianzhi Dong, Weijie Yu, Qian Wang, Daoxin Front Genet Genetics Tumor metastasis and invasion are the main impediments to lung adenocarcinoma successful treatment. Previous studies demonstrate that chemotherapeutic agents can elevate the malignancy of cancer cells other than their therapeutic effects. In this study, the effects of transient low-dose cisplatin treatment on the malignant development of lung adenocarcinoma cells (A549) were detected, and the underlying epigenetic mechanisms were investigated. The findings showed that A549 cells exhibited epithelial-mesenchymal transition (EMT)-like phenotype along with malignant progression under the transient low-dose cisplatin treatment. Meanwhile, low-dose cisplatin was found to induce contactin-1 (CNTN-1) upregulation in A549 cells. Subsequently, we found that further overexpressing CNTN-1 in A549 cells obviously activated the EMT process in vitro and in vivo, and caused malignant development of A549 cells in vitro. Taken together, we conclude that low-dose cisplatin can activate the EMT process and resulting malignant progression through upregulating CNTN-1 in A549 cells. The findings provided new evidence that a low concentration of chemotherapeutic agents could facilitate the malignancy of carcinoma cells via activating the EMT process other than their therapeutic effects. Frontiers Media S.A. 2022-05-27 /pmc/articles/PMC9196332/ /pubmed/35711928 http://dx.doi.org/10.3389/fgene.2022.891665 Text en Copyright © 2022 Zhang, Li, Lan, Li, Du, Dong, Yu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhang, Ruijie
Li, Shengjin
Lan, Jian
Li, Changyi
Du, Xianzhi
Dong, Weijie
Yu, Qian
Wang, Daoxin
CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition
title CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition
title_full CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition
title_fullStr CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition
title_full_unstemmed CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition
title_short CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition
title_sort cntn-1 upregulation induced by low-dose cisplatin promotes malignant progression of lung adenocarcinoma cells via activation of epithelial-mesenchymal transition
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196332/
https://www.ncbi.nlm.nih.gov/pubmed/35711928
http://dx.doi.org/10.3389/fgene.2022.891665
work_keys_str_mv AT zhangruijie cntn1upregulationinducedbylowdosecisplatinpromotesmalignantprogressionoflungadenocarcinomacellsviaactivationofepithelialmesenchymaltransition
AT lishengjin cntn1upregulationinducedbylowdosecisplatinpromotesmalignantprogressionoflungadenocarcinomacellsviaactivationofepithelialmesenchymaltransition
AT lanjian cntn1upregulationinducedbylowdosecisplatinpromotesmalignantprogressionoflungadenocarcinomacellsviaactivationofepithelialmesenchymaltransition
AT lichangyi cntn1upregulationinducedbylowdosecisplatinpromotesmalignantprogressionoflungadenocarcinomacellsviaactivationofepithelialmesenchymaltransition
AT duxianzhi cntn1upregulationinducedbylowdosecisplatinpromotesmalignantprogressionoflungadenocarcinomacellsviaactivationofepithelialmesenchymaltransition
AT dongweijie cntn1upregulationinducedbylowdosecisplatinpromotesmalignantprogressionoflungadenocarcinomacellsviaactivationofepithelialmesenchymaltransition
AT yuqian cntn1upregulationinducedbylowdosecisplatinpromotesmalignantprogressionoflungadenocarcinomacellsviaactivationofepithelialmesenchymaltransition
AT wangdaoxin cntn1upregulationinducedbylowdosecisplatinpromotesmalignantprogressionoflungadenocarcinomacellsviaactivationofepithelialmesenchymaltransition