Monitoring basal autophagy in the retina utilizing CAG-mRFP-EGFP-MAP1LC3B reporter mouse: technical and biological considerations

We describe the utility of a tandem-tagged autophagy reporter mouse model (CAG-RFP-EGFP-MAP1LC3B) in investigating basal macroautophagic/autophagic flux in the neural retina. Western blot, in situ hybridization, immunohistochemistry, and confocal microscopy showed that CAG promoter-driven expression...

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Detalles Bibliográficos
Autores principales: Ramachandra Rao, Sriganesh, Fliesler, Steven J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196719/
https://www.ncbi.nlm.nih.gov/pubmed/34674604
http://dx.doi.org/10.1080/15548627.2021.1969634

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196719/
https://www.ncbi.nlm.nih.gov/pubmed/34674604
http://dx.doi.org/10.1080/15548627.2021.1969634

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