Spinach-based RNA mimicking GFP in plant cells

Spinach RNA-mimicking GFP (S-RMG) has been successfully used to monitor cellular RNAs including microRNAs in bacterium, yeast, and human cells. However, S-RMG has not been established in plants. In this study, we found that like bacterial, yeast, and human cellular tRNAs, plant tRNAs such as tRNA(Ly...

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Autores principales: Yu, Zhiming, Wang, Yue, Mei, Fengling, Yan, Haiting, Jin, Zhenhui, Zhang, Pengcheng, Zhang, Xian, Tör, Mahmut, Jackson, Stephen, Shi, Nongnong, Hong, Yiguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197860/
https://www.ncbi.nlm.nih.gov/pubmed/35267109
http://dx.doi.org/10.1007/s10142-022-00835-x
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author Yu, Zhiming
Wang, Yue
Mei, Fengling
Yan, Haiting
Jin, Zhenhui
Zhang, Pengcheng
Zhang, Xian
Tör, Mahmut
Jackson, Stephen
Shi, Nongnong
Hong, Yiguo
author_facet Yu, Zhiming
Wang, Yue
Mei, Fengling
Yan, Haiting
Jin, Zhenhui
Zhang, Pengcheng
Zhang, Xian
Tör, Mahmut
Jackson, Stephen
Shi, Nongnong
Hong, Yiguo
author_sort Yu, Zhiming
collection PubMed
description Spinach RNA-mimicking GFP (S-RMG) has been successfully used to monitor cellular RNAs including microRNAs in bacterium, yeast, and human cells. However, S-RMG has not been established in plants. In this study, we found that like bacterial, yeast, and human cellular tRNAs, plant tRNAs such as tRNA(Lys) can protect and/or stabilize the Spinach RNA aptamer interaction with the fluorophore DFHBI enabling detectable levels of green fluorescence to be emitted. The tRNA(Lys)-Spinach-tRNA(Lys), once delivered into “chloroplast-free” onion epidermal cells can emit strong green fluorescence in the presence of DFHBI. Our results demonstrate for the first time that Spinach-based RNA visualization has the potential for in vivo monitoring of RNAs in plant cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10142-022-00835-x.
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spelling pubmed-91978602022-06-16 Spinach-based RNA mimicking GFP in plant cells Yu, Zhiming Wang, Yue Mei, Fengling Yan, Haiting Jin, Zhenhui Zhang, Pengcheng Zhang, Xian Tör, Mahmut Jackson, Stephen Shi, Nongnong Hong, Yiguo Funct Integr Genomics Short Communication Spinach RNA-mimicking GFP (S-RMG) has been successfully used to monitor cellular RNAs including microRNAs in bacterium, yeast, and human cells. However, S-RMG has not been established in plants. In this study, we found that like bacterial, yeast, and human cellular tRNAs, plant tRNAs such as tRNA(Lys) can protect and/or stabilize the Spinach RNA aptamer interaction with the fluorophore DFHBI enabling detectable levels of green fluorescence to be emitted. The tRNA(Lys)-Spinach-tRNA(Lys), once delivered into “chloroplast-free” onion epidermal cells can emit strong green fluorescence in the presence of DFHBI. Our results demonstrate for the first time that Spinach-based RNA visualization has the potential for in vivo monitoring of RNAs in plant cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10142-022-00835-x. Springer Berlin Heidelberg 2022-03-10 2022 /pmc/articles/PMC9197860/ /pubmed/35267109 http://dx.doi.org/10.1007/s10142-022-00835-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Short Communication
Yu, Zhiming
Wang, Yue
Mei, Fengling
Yan, Haiting
Jin, Zhenhui
Zhang, Pengcheng
Zhang, Xian
Tör, Mahmut
Jackson, Stephen
Shi, Nongnong
Hong, Yiguo
Spinach-based RNA mimicking GFP in plant cells
title Spinach-based RNA mimicking GFP in plant cells
title_full Spinach-based RNA mimicking GFP in plant cells
title_fullStr Spinach-based RNA mimicking GFP in plant cells
title_full_unstemmed Spinach-based RNA mimicking GFP in plant cells
title_short Spinach-based RNA mimicking GFP in plant cells
title_sort spinach-based rna mimicking gfp in plant cells
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197860/
https://www.ncbi.nlm.nih.gov/pubmed/35267109
http://dx.doi.org/10.1007/s10142-022-00835-x
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