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Oxygen consumption and acid secretion in isolated gas gland cells of the European eel Anguilla anguilla
Swimbladder gas gland cells are known to produce lactic acid required for the acidification of swimbladder blood and decreasing the oxygen carrying capacity of swimbladder blood, i.e., the onset of the Root effect. Gas gland cells have also been shown to metabolize glucose via the pentose phosphate...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197889/ https://www.ncbi.nlm.nih.gov/pubmed/35289381 http://dx.doi.org/10.1007/s00360-022-01432-x |
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author | Drechsel, Victoria Schneebauer, Gabriel Sandbichler, Adolf M. Fiechtner, Birgit Pelster, Bernd |
author_facet | Drechsel, Victoria Schneebauer, Gabriel Sandbichler, Adolf M. Fiechtner, Birgit Pelster, Bernd |
author_sort | Drechsel, Victoria |
collection | PubMed |
description | Swimbladder gas gland cells are known to produce lactic acid required for the acidification of swimbladder blood and decreasing the oxygen carrying capacity of swimbladder blood, i.e., the onset of the Root effect. Gas gland cells have also been shown to metabolize glucose via the pentose phosphate shunt, but the role of the pentose phosphate shunt for acid secretion has not yet been evaluated. Similarly, aerobic metabolism of gas gland cells has been largely neglected so far. In the present study, we therefore simultaneously assessed the role of glycolysis and of the pentose phosphate shunt for acid secretion and recorded oxygen consumption of isolated swimbladder gas gland cells of the European eel. Presence of glucose was essential for acid secretion, and at glucose concentrations of about 1.5 mmol l(−1) acid secretion of gas gland cells reached a maximum, indicating that glucose concentrations in swimbladder blood should not be limiting acid production and secretion under physiological conditions. The data revealed that most of the acid was produced in the glycolytic pathway, but a significant fraction was also contributed by the pentose phosphate shunt. Addition of glucose to gas gland cells incubated in a glucose-free medium resulted in a reduction of oxygen uptake. Inhibition of mitochondrial respiration significantly reduced oxygen consumption, but a fraction of mitochondria-independent respiration remained in presence of rotenone and antimycin A. In the presence of glucose, application of either iodo-acetate inhibiting glycolysis or 6-AN inhibiting the pentose phosphate shunt did not significantly affect oxygen uptake, indicating an independent regulation of oxidative phosphorylation and of acid production. Inhibition of the muscarinic acetylcholine receptor caused a slight elevation in acid secretion, while forskolin caused a concentration-dependent reduction in acid secretion, indicating muscarinic and c-AMP-dependent control of acid secretion in gas gland cells. |
format | Online Article Text |
id | pubmed-9197889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-91978892022-06-16 Oxygen consumption and acid secretion in isolated gas gland cells of the European eel Anguilla anguilla Drechsel, Victoria Schneebauer, Gabriel Sandbichler, Adolf M. Fiechtner, Birgit Pelster, Bernd J Comp Physiol B Original Paper Swimbladder gas gland cells are known to produce lactic acid required for the acidification of swimbladder blood and decreasing the oxygen carrying capacity of swimbladder blood, i.e., the onset of the Root effect. Gas gland cells have also been shown to metabolize glucose via the pentose phosphate shunt, but the role of the pentose phosphate shunt for acid secretion has not yet been evaluated. Similarly, aerobic metabolism of gas gland cells has been largely neglected so far. In the present study, we therefore simultaneously assessed the role of glycolysis and of the pentose phosphate shunt for acid secretion and recorded oxygen consumption of isolated swimbladder gas gland cells of the European eel. Presence of glucose was essential for acid secretion, and at glucose concentrations of about 1.5 mmol l(−1) acid secretion of gas gland cells reached a maximum, indicating that glucose concentrations in swimbladder blood should not be limiting acid production and secretion under physiological conditions. The data revealed that most of the acid was produced in the glycolytic pathway, but a significant fraction was also contributed by the pentose phosphate shunt. Addition of glucose to gas gland cells incubated in a glucose-free medium resulted in a reduction of oxygen uptake. Inhibition of mitochondrial respiration significantly reduced oxygen consumption, but a fraction of mitochondria-independent respiration remained in presence of rotenone and antimycin A. In the presence of glucose, application of either iodo-acetate inhibiting glycolysis or 6-AN inhibiting the pentose phosphate shunt did not significantly affect oxygen uptake, indicating an independent regulation of oxidative phosphorylation and of acid production. Inhibition of the muscarinic acetylcholine receptor caused a slight elevation in acid secretion, while forskolin caused a concentration-dependent reduction in acid secretion, indicating muscarinic and c-AMP-dependent control of acid secretion in gas gland cells. Springer Berlin Heidelberg 2022-03-14 2022 /pmc/articles/PMC9197889/ /pubmed/35289381 http://dx.doi.org/10.1007/s00360-022-01432-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Paper Drechsel, Victoria Schneebauer, Gabriel Sandbichler, Adolf M. Fiechtner, Birgit Pelster, Bernd Oxygen consumption and acid secretion in isolated gas gland cells of the European eel Anguilla anguilla |
title | Oxygen consumption and acid secretion in isolated gas gland cells of the European eel Anguilla anguilla |
title_full | Oxygen consumption and acid secretion in isolated gas gland cells of the European eel Anguilla anguilla |
title_fullStr | Oxygen consumption and acid secretion in isolated gas gland cells of the European eel Anguilla anguilla |
title_full_unstemmed | Oxygen consumption and acid secretion in isolated gas gland cells of the European eel Anguilla anguilla |
title_short | Oxygen consumption and acid secretion in isolated gas gland cells of the European eel Anguilla anguilla |
title_sort | oxygen consumption and acid secretion in isolated gas gland cells of the european eel anguilla anguilla |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197889/ https://www.ncbi.nlm.nih.gov/pubmed/35289381 http://dx.doi.org/10.1007/s00360-022-01432-x |
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