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Gut mucosa dissociation protocols influence cell type proportions and single-cell gene expression levels

Single-cell RNA sequencing (scRNA-seq) has revolutionized the study of the cellular landscape of organs. Most single-cell protocols require fresh material, which limits sample size per experiment, and consequently, introduces batch effects. This is especially true for samples acquired through comple...

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Detalles Bibliográficos
Autores principales:	Uniken Venema, Werna T. C., Ramírez-Sánchez, Aarón D., Bigaeva, Emilia, Withoff, Sebo, Jonkers, Iris, McIntyre, Rebecca E., Ghouraba, Mennatallah, Raine, Tim, Weersma, Rinse K., Franke, Lude, Festen, Eleonora A. M., van der Wijst, Monique G. P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197976/ https://www.ncbi.nlm.nih.gov/pubmed/35701452 http://dx.doi.org/10.1038/s41598-022-13812-y

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