An optimized protocol for single nuclei isolation from clinical biopsies for RNA-seq

Single nuclei RNA sequencing (snRNA-seq) has evolved as a powerful tool to study complex human diseases. Single cell resolution enables the study of novel cell types, biological processes, cell trajectories, and cell–cell signaling pathways. snRNA-seq largely relies on the dissociation of intact nuc...

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Detalles Bibliográficos
Autores principales: Rousselle, Thomas V., McDaniels, Jennifer M., Shetty, Amol C., Bardhi, Elissa, Maluf, Daniel G., Mas, Valeria R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198012/
https://www.ncbi.nlm.nih.gov/pubmed/35701599
http://dx.doi.org/10.1038/s41598-022-14099-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198012/
https://www.ncbi.nlm.nih.gov/pubmed/35701599
http://dx.doi.org/10.1038/s41598-022-14099-9

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