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Production Ganoderma lucidum extract nanoparticles by expansion of supercritical fluid solution and evaluation of the antioxidant ability
Due to the growing human tendency to treat with natural substances, fungi such as Ganoderma lucidum can be a good source to meet this need. Effectiveness, ease of use and a rich source of active ingredients such as ganoderic acids have caused G. lucidum to be considered in the pharmaceutical and foo...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198024/ https://www.ncbi.nlm.nih.gov/pubmed/35701498 http://dx.doi.org/10.1038/s41598-022-13727-8 |
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author | Karimi, Mehrnaz Raofie, Farhad Karimi, Mehrdad |
author_facet | Karimi, Mehrnaz Raofie, Farhad Karimi, Mehrdad |
author_sort | Karimi, Mehrnaz |
collection | PubMed |
description | Due to the growing human tendency to treat with natural substances, fungi such as Ganoderma lucidum can be a good source to meet this need. Effectiveness, ease of use and a rich source of active ingredients such as ganoderic acids have caused G. lucidum to be considered in the pharmaceutical and food industries. In this project, G. lucidum was applied to extraction using supercritical carbon dioxide. Then expansion of supercritical fluid solution (ESS) was used as, novel, repeatable and green method to yield nanoparticles from G.lucidum extract. The response surface method was used to improve the Extraction efficiency, antioxidant activity, and improving the nanoparticles production status. Optimal conditions were observed at the extraction step by setting pressure at 27.5 MPa, dynamic time of 46 min, and modifier volume of 162 μL. The optimum point for the production of nanoparticles was obtained as follows: pressure drop at 25 MPa, 20 min for collection time, and 40° C for temperature. Under these conditions, the size and count were 86.13 nm, and 98, respectively. Nanoparticles were analyzed by FESM and, the DPPH was used for antioxidant activity evaluation. The LC–MS identified various ganoderic acids from G.lucidum that are famous to be highly oxygenated triterpenoids. |
format | Online Article Text |
id | pubmed-9198024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-91980242022-06-16 Production Ganoderma lucidum extract nanoparticles by expansion of supercritical fluid solution and evaluation of the antioxidant ability Karimi, Mehrnaz Raofie, Farhad Karimi, Mehrdad Sci Rep Article Due to the growing human tendency to treat with natural substances, fungi such as Ganoderma lucidum can be a good source to meet this need. Effectiveness, ease of use and a rich source of active ingredients such as ganoderic acids have caused G. lucidum to be considered in the pharmaceutical and food industries. In this project, G. lucidum was applied to extraction using supercritical carbon dioxide. Then expansion of supercritical fluid solution (ESS) was used as, novel, repeatable and green method to yield nanoparticles from G.lucidum extract. The response surface method was used to improve the Extraction efficiency, antioxidant activity, and improving the nanoparticles production status. Optimal conditions were observed at the extraction step by setting pressure at 27.5 MPa, dynamic time of 46 min, and modifier volume of 162 μL. The optimum point for the production of nanoparticles was obtained as follows: pressure drop at 25 MPa, 20 min for collection time, and 40° C for temperature. Under these conditions, the size and count were 86.13 nm, and 98, respectively. Nanoparticles were analyzed by FESM and, the DPPH was used for antioxidant activity evaluation. The LC–MS identified various ganoderic acids from G.lucidum that are famous to be highly oxygenated triterpenoids. Nature Publishing Group UK 2022-06-14 /pmc/articles/PMC9198024/ /pubmed/35701498 http://dx.doi.org/10.1038/s41598-022-13727-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Karimi, Mehrnaz Raofie, Farhad Karimi, Mehrdad Production Ganoderma lucidum extract nanoparticles by expansion of supercritical fluid solution and evaluation of the antioxidant ability |
title | Production Ganoderma lucidum extract nanoparticles by expansion of supercritical fluid solution and evaluation of the antioxidant ability |
title_full | Production Ganoderma lucidum extract nanoparticles by expansion of supercritical fluid solution and evaluation of the antioxidant ability |
title_fullStr | Production Ganoderma lucidum extract nanoparticles by expansion of supercritical fluid solution and evaluation of the antioxidant ability |
title_full_unstemmed | Production Ganoderma lucidum extract nanoparticles by expansion of supercritical fluid solution and evaluation of the antioxidant ability |
title_short | Production Ganoderma lucidum extract nanoparticles by expansion of supercritical fluid solution and evaluation of the antioxidant ability |
title_sort | production ganoderma lucidum extract nanoparticles by expansion of supercritical fluid solution and evaluation of the antioxidant ability |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198024/ https://www.ncbi.nlm.nih.gov/pubmed/35701498 http://dx.doi.org/10.1038/s41598-022-13727-8 |
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