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SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish

Precise genetic modifications in model organisms are essential for biomedical research. The recent development of PAM-less base editors makes it possible to assess the functional impact and pathogenicity of nucleotide mutations in animals. Here we first optimize SpG and SpRY systems in zebrafish by...

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Detalles Bibliográficos
Autores principales:	Liang, Fang, Zhang, Yu, Li, Lin, Yang, Yexin, Fei, Ji-Feng, Liu, Yanmei, Qin, Wei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198057/ https://www.ncbi.nlm.nih.gov/pubmed/35701400 http://dx.doi.org/10.1038/s41467-022-31034-8

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