Cargando…
The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca
The aerobic, thermophilic Actinobacterium, Thermobifida fusca has been proposed as an organism to be used for the efficient conversion of plant biomass to fatty acid-derived precursors of biofuels or biorenewable chemicals. Despite the potential of T. fusca to catabolize plant biomass, there is rema...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198275/ https://www.ncbi.nlm.nih.gov/pubmed/35720111 http://dx.doi.org/10.3389/fmolb.2022.896226 |
_version_ | 1784727576595398656 |
---|---|
author | Winkelman, Dirk C. Nikolau, Basil J. |
author_facet | Winkelman, Dirk C. Nikolau, Basil J. |
author_sort | Winkelman, Dirk C. |
collection | PubMed |
description | The aerobic, thermophilic Actinobacterium, Thermobifida fusca has been proposed as an organism to be used for the efficient conversion of plant biomass to fatty acid-derived precursors of biofuels or biorenewable chemicals. Despite the potential of T. fusca to catabolize plant biomass, there is remarkably little data available concerning the natural ability of this organism to produce fatty acids. Therefore, we determined the fatty acids that T. fusca produces when it is grown on different carbon sources (i.e., glucose, cellobiose, cellulose and avicel) and at two different growth temperatures, namely at the optimal growth temperature of 50°C and at a suboptimal temperature of 37°C. These analyses establish that T. fusca produces a combination of linear and branched chain fatty acids (BCFAs), including iso-, anteiso-, and 10-methyl BCFAs that range between 14- and 18-carbons in length. Although different carbon sources and growth temperatures both quantitatively and qualitatively affect the fatty acid profiles produced by T. fusca, growth temperature is the greater modifier of these traits. Additionally, genome scanning enabled the identification of many of the fatty acid biosynthetic genes encoded by T. fusca. |
format | Online Article Text |
id | pubmed-9198275 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91982752022-06-16 The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca Winkelman, Dirk C. Nikolau, Basil J. Front Mol Biosci Molecular Biosciences The aerobic, thermophilic Actinobacterium, Thermobifida fusca has been proposed as an organism to be used for the efficient conversion of plant biomass to fatty acid-derived precursors of biofuels or biorenewable chemicals. Despite the potential of T. fusca to catabolize plant biomass, there is remarkably little data available concerning the natural ability of this organism to produce fatty acids. Therefore, we determined the fatty acids that T. fusca produces when it is grown on different carbon sources (i.e., glucose, cellobiose, cellulose and avicel) and at two different growth temperatures, namely at the optimal growth temperature of 50°C and at a suboptimal temperature of 37°C. These analyses establish that T. fusca produces a combination of linear and branched chain fatty acids (BCFAs), including iso-, anteiso-, and 10-methyl BCFAs that range between 14- and 18-carbons in length. Although different carbon sources and growth temperatures both quantitatively and qualitatively affect the fatty acid profiles produced by T. fusca, growth temperature is the greater modifier of these traits. Additionally, genome scanning enabled the identification of many of the fatty acid biosynthetic genes encoded by T. fusca. Frontiers Media S.A. 2022-06-01 /pmc/articles/PMC9198275/ /pubmed/35720111 http://dx.doi.org/10.3389/fmolb.2022.896226 Text en Copyright © 2022 Winkelman and Nikolau. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Winkelman, Dirk C. Nikolau, Basil J. The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca |
title | The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca
|
title_full | The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca
|
title_fullStr | The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca
|
title_full_unstemmed | The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca
|
title_short | The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca
|
title_sort | effects of carbon source and growth temperature on the fatty acid profiles of thermobifida fusca |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198275/ https://www.ncbi.nlm.nih.gov/pubmed/35720111 http://dx.doi.org/10.3389/fmolb.2022.896226 |
work_keys_str_mv | AT winkelmandirkc theeffectsofcarbonsourceandgrowthtemperatureonthefattyacidprofilesofthermobifidafusca AT nikolaubasilj theeffectsofcarbonsourceandgrowthtemperatureonthefattyacidprofilesofthermobifidafusca AT winkelmandirkc effectsofcarbonsourceandgrowthtemperatureonthefattyacidprofilesofthermobifidafusca AT nikolaubasilj effectsofcarbonsourceandgrowthtemperatureonthefattyacidprofilesofthermobifidafusca |