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Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers

Triticum timopheevii (2n = 28, A(t)A(t)GG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat Triticum aestivum (2n = 42, AABBDD) for various important agronomic traits. A breeding scheme that propagated advanced backcrossed populations of...

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Autores principales: King, Julie, Grewal, Surbhi, Othmeni, Manel, Coombes, Benedict, Yang, Cai-yun, Walter, Nicola, Ashling, Stephen, Scholefield, Duncan, Walker, Jack, Hubbart-Edwards, Stella, Hall, Anthony, King, Ian Phillip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198554/
https://www.ncbi.nlm.nih.gov/pubmed/35720607
http://dx.doi.org/10.3389/fpls.2022.919519
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author King, Julie
Grewal, Surbhi
Othmeni, Manel
Coombes, Benedict
Yang, Cai-yun
Walter, Nicola
Ashling, Stephen
Scholefield, Duncan
Walker, Jack
Hubbart-Edwards, Stella
Hall, Anthony
King, Ian Phillip
author_facet King, Julie
Grewal, Surbhi
Othmeni, Manel
Coombes, Benedict
Yang, Cai-yun
Walter, Nicola
Ashling, Stephen
Scholefield, Duncan
Walker, Jack
Hubbart-Edwards, Stella
Hall, Anthony
King, Ian Phillip
author_sort King, Julie
collection PubMed
description Triticum timopheevii (2n = 28, A(t)A(t)GG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat Triticum aestivum (2n = 42, AABBDD) for various important agronomic traits. A breeding scheme that propagated advanced backcrossed populations of wheat-T. timopheevii introgression lines through further backcrossing and self-fertilisation resulted in the generation of 99 introgression lines (ILs) that carried 309 homozygous segments from the A(t) and G subgenomes of T. timopheevii. These introgressions contained 89 and 74 unique segments from the A(t) and G subgenomes, respectively. These overlapping segments covered 98.9% of the T. timopheevii genome that has now been introgressed into bread wheat cv. Paragon including the entirety of all T. timopheevii chromosomes via varying sized segments except for chromosomes 3A(t), 4G, and 6G. Homozygous ILs contained between one and eight of these introgressions with an average of three per introgression line. These homozygous introgressions were detected through the development of a set of 480 chromosome-specific Kompetitive allele specific PCR (KASP) markers that are well-distributed across the wheat genome. Of these, 149 were developed in this study based on single nucleotide polymorphisms (SNPs) discovered through whole genome sequencing of T. timopheevii. A majority of these KASP markers were also found to be T. timopheevii subgenome specific with 182 detecting A(t) subgenome and 275 detecting G subgenome segments. These markers showed that 98% of the A(t) segments had recombined with the A genome of wheat and 74% of the G genome segments had recombined with the B genome of wheat with the rest recombining with the D genome of wheat. These results were validated through multi-colour in situ hybridisation analysis. Together these homozygous wheat-T. timopheevii ILs and chromosome-specific KASP markers provide an invaluable resource to wheat breeders for trait discovery to combat biotic and abiotic stress factors affecting wheat production due to climate change.
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spelling pubmed-91985542022-06-16 Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers King, Julie Grewal, Surbhi Othmeni, Manel Coombes, Benedict Yang, Cai-yun Walter, Nicola Ashling, Stephen Scholefield, Duncan Walker, Jack Hubbart-Edwards, Stella Hall, Anthony King, Ian Phillip Front Plant Sci Plant Science Triticum timopheevii (2n = 28, A(t)A(t)GG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat Triticum aestivum (2n = 42, AABBDD) for various important agronomic traits. A breeding scheme that propagated advanced backcrossed populations of wheat-T. timopheevii introgression lines through further backcrossing and self-fertilisation resulted in the generation of 99 introgression lines (ILs) that carried 309 homozygous segments from the A(t) and G subgenomes of T. timopheevii. These introgressions contained 89 and 74 unique segments from the A(t) and G subgenomes, respectively. These overlapping segments covered 98.9% of the T. timopheevii genome that has now been introgressed into bread wheat cv. Paragon including the entirety of all T. timopheevii chromosomes via varying sized segments except for chromosomes 3A(t), 4G, and 6G. Homozygous ILs contained between one and eight of these introgressions with an average of three per introgression line. These homozygous introgressions were detected through the development of a set of 480 chromosome-specific Kompetitive allele specific PCR (KASP) markers that are well-distributed across the wheat genome. Of these, 149 were developed in this study based on single nucleotide polymorphisms (SNPs) discovered through whole genome sequencing of T. timopheevii. A majority of these KASP markers were also found to be T. timopheevii subgenome specific with 182 detecting A(t) subgenome and 275 detecting G subgenome segments. These markers showed that 98% of the A(t) segments had recombined with the A genome of wheat and 74% of the G genome segments had recombined with the B genome of wheat with the rest recombining with the D genome of wheat. These results were validated through multi-colour in situ hybridisation analysis. Together these homozygous wheat-T. timopheevii ILs and chromosome-specific KASP markers provide an invaluable resource to wheat breeders for trait discovery to combat biotic and abiotic stress factors affecting wheat production due to climate change. Frontiers Media S.A. 2022-06-01 /pmc/articles/PMC9198554/ /pubmed/35720607 http://dx.doi.org/10.3389/fpls.2022.919519 Text en Copyright © 2022 King, Grewal, Othmeni, Coombes, Yang, Walter, Ashling, Scholefield, Walker, Hubbart-Edwards, Hall and King. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
King, Julie
Grewal, Surbhi
Othmeni, Manel
Coombes, Benedict
Yang, Cai-yun
Walter, Nicola
Ashling, Stephen
Scholefield, Duncan
Walker, Jack
Hubbart-Edwards, Stella
Hall, Anthony
King, Ian Phillip
Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers
title Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers
title_full Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers
title_fullStr Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers
title_full_unstemmed Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers
title_short Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers
title_sort introgression of the triticum timopheevii genome into wheat detected by chromosome-specific kompetitive allele specific pcr markers
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198554/
https://www.ncbi.nlm.nih.gov/pubmed/35720607
http://dx.doi.org/10.3389/fpls.2022.919519
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