Cargando…

A Diaporthe Fungal Endophyte From a Wild Grass Improves Growth and Salinity Tolerance of Tritordeum and Perennial Ryegrass

Some microbiome components can provide functions that extend the capabilities of plants, increasing the environmental adaptability and performance of holobionts. Festuca rubra subsp. pruinosa is a perennial grass adapted to rocky sea cliffs, where soil and nutrients are very limited, and exposure to...

Descripción completa

Detalles Bibliográficos
Autores principales: Toghueo, Rufin M. K., Zabalgogeazcoa, Iñigo, Pereira, Eric C., Vazquez de Aldana, Beatriz R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198640/
https://www.ncbi.nlm.nih.gov/pubmed/35720593
http://dx.doi.org/10.3389/fpls.2022.896755
_version_ 1784727671689707520
author Toghueo, Rufin M. K.
Zabalgogeazcoa, Iñigo
Pereira, Eric C.
Vazquez de Aldana, Beatriz R.
author_facet Toghueo, Rufin M. K.
Zabalgogeazcoa, Iñigo
Pereira, Eric C.
Vazquez de Aldana, Beatriz R.
author_sort Toghueo, Rufin M. K.
collection PubMed
description Some microbiome components can provide functions that extend the capabilities of plants, increasing the environmental adaptability and performance of holobionts. Festuca rubra subsp. pruinosa is a perennial grass adapted to rocky sea cliffs, where soil and nutrients are very limited, and exposure to salinity is continuous. This study aimed to investigate if a Diaporthe fungal endophyte belonging to the core microbiome of Festuca rubra roots could improve the performance of two agricultural grasses. In a greenhouse experiment, plants of tritordeum (Triticum durum x Hordeum chilense) and perennial ryegrass (Lolium perenne) were inoculated with Diaporthe strain EB4 and subjected to two salinity conditions (0 and 200 mM NaCl). Biomass production, mineral elements, proline, hormone profiles, antioxidant capacity, and total phenolic compounds were examined in plants, and fungal functions potentially related to the promotion of plant growth were determined. The inoculation with Diaporthe promoted plant growth of both grasses, increasing leaf biomass (84% in tritordeum and 29% in perennial ryegrass), root biomass, nutrient content (N, Ca, Mg, and Fe), and the production of indole 3-acetic acid, regardless of the salinity treatment. Improved growth and nutrient uptake might occur because Diaporthe produces several extracellular enzymes capable of recycling organic nutrient pools. In addition, the fungus produced indole 3-acetic acid in vitro and modulated the production of this phytohormone in the plant. Under salinity, the activity of Diaporthe ameliorated the stress, increasing proline, nutrient uptake in roots, gibberellins, and indole 3-acetic acid, which in turn results into improved growth. Thus, this fungus can transfer to alternative hosts some advantages useful at its original habitat.
format Online
Article
Text
id pubmed-9198640
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91986402022-06-16 A Diaporthe Fungal Endophyte From a Wild Grass Improves Growth and Salinity Tolerance of Tritordeum and Perennial Ryegrass Toghueo, Rufin M. K. Zabalgogeazcoa, Iñigo Pereira, Eric C. Vazquez de Aldana, Beatriz R. Front Plant Sci Plant Science Some microbiome components can provide functions that extend the capabilities of plants, increasing the environmental adaptability and performance of holobionts. Festuca rubra subsp. pruinosa is a perennial grass adapted to rocky sea cliffs, where soil and nutrients are very limited, and exposure to salinity is continuous. This study aimed to investigate if a Diaporthe fungal endophyte belonging to the core microbiome of Festuca rubra roots could improve the performance of two agricultural grasses. In a greenhouse experiment, plants of tritordeum (Triticum durum x Hordeum chilense) and perennial ryegrass (Lolium perenne) were inoculated with Diaporthe strain EB4 and subjected to two salinity conditions (0 and 200 mM NaCl). Biomass production, mineral elements, proline, hormone profiles, antioxidant capacity, and total phenolic compounds were examined in plants, and fungal functions potentially related to the promotion of plant growth were determined. The inoculation with Diaporthe promoted plant growth of both grasses, increasing leaf biomass (84% in tritordeum and 29% in perennial ryegrass), root biomass, nutrient content (N, Ca, Mg, and Fe), and the production of indole 3-acetic acid, regardless of the salinity treatment. Improved growth and nutrient uptake might occur because Diaporthe produces several extracellular enzymes capable of recycling organic nutrient pools. In addition, the fungus produced indole 3-acetic acid in vitro and modulated the production of this phytohormone in the plant. Under salinity, the activity of Diaporthe ameliorated the stress, increasing proline, nutrient uptake in roots, gibberellins, and indole 3-acetic acid, which in turn results into improved growth. Thus, this fungus can transfer to alternative hosts some advantages useful at its original habitat. Frontiers Media S.A. 2022-05-27 /pmc/articles/PMC9198640/ /pubmed/35720593 http://dx.doi.org/10.3389/fpls.2022.896755 Text en Copyright © 2022 Toghueo, Zabalgogeazcoa, Pereira and Vazquez de Aldana. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Toghueo, Rufin M. K.
Zabalgogeazcoa, Iñigo
Pereira, Eric C.
Vazquez de Aldana, Beatriz R.
A Diaporthe Fungal Endophyte From a Wild Grass Improves Growth and Salinity Tolerance of Tritordeum and Perennial Ryegrass
title A Diaporthe Fungal Endophyte From a Wild Grass Improves Growth and Salinity Tolerance of Tritordeum and Perennial Ryegrass
title_full A Diaporthe Fungal Endophyte From a Wild Grass Improves Growth and Salinity Tolerance of Tritordeum and Perennial Ryegrass
title_fullStr A Diaporthe Fungal Endophyte From a Wild Grass Improves Growth and Salinity Tolerance of Tritordeum and Perennial Ryegrass
title_full_unstemmed A Diaporthe Fungal Endophyte From a Wild Grass Improves Growth and Salinity Tolerance of Tritordeum and Perennial Ryegrass
title_short A Diaporthe Fungal Endophyte From a Wild Grass Improves Growth and Salinity Tolerance of Tritordeum and Perennial Ryegrass
title_sort diaporthe fungal endophyte from a wild grass improves growth and salinity tolerance of tritordeum and perennial ryegrass
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198640/
https://www.ncbi.nlm.nih.gov/pubmed/35720593
http://dx.doi.org/10.3389/fpls.2022.896755
work_keys_str_mv AT toghueorufinmk adiaporthefungalendophytefromawildgrassimprovesgrowthandsalinitytoleranceoftritordeumandperennialryegrass
AT zabalgogeazcoainigo adiaporthefungalendophytefromawildgrassimprovesgrowthandsalinitytoleranceoftritordeumandperennialryegrass
AT pereiraericc adiaporthefungalendophytefromawildgrassimprovesgrowthandsalinitytoleranceoftritordeumandperennialryegrass
AT vazquezdealdanabeatrizr adiaporthefungalendophytefromawildgrassimprovesgrowthandsalinitytoleranceoftritordeumandperennialryegrass
AT toghueorufinmk diaporthefungalendophytefromawildgrassimprovesgrowthandsalinitytoleranceoftritordeumandperennialryegrass
AT zabalgogeazcoainigo diaporthefungalendophytefromawildgrassimprovesgrowthandsalinitytoleranceoftritordeumandperennialryegrass
AT pereiraericc diaporthefungalendophytefromawildgrassimprovesgrowthandsalinitytoleranceoftritordeumandperennialryegrass
AT vazquezdealdanabeatrizr diaporthefungalendophytefromawildgrassimprovesgrowthandsalinitytoleranceoftritordeumandperennialryegrass