Residues located in the primase domain of the bacteriophage T7 primase-helicase are essential for loading the hexameric complex onto DNA

The T7 primase-helicase plays a pivotal role in the replication of T7 DNA. Using affinity isolation of peptide–nucleic acid crosslinks and mass spectrometry, we identify protein regions in the primase-helicase and T7 DNA polymerase that form contacts with the RNA primer and DNA template. The contact...

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Detalles Bibliográficos
Autores principales: Hernandez, Alfredo J., Lee, Seung-Joo, Thompson, Noah J., Griffith, Jack D., Richardson, Charles C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198812/
https://www.ncbi.nlm.nih.gov/pubmed/35500649
http://dx.doi.org/10.1016/j.jbc.2022.101996

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