Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus

Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a...

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Detalles Bibliográficos
Autores principales: Domingo-Reinés, Joan, Martínez-Navajas, Gonzalo, Montes, Rosa, Lamolda, Mar, Simón, Iris, Castaño, Julio, Ríos-Pelegrina, Rosa, Lopez-Hidalgo, Javier Luis, García del Moral, Raimundo, Marchal, Juan A., Real, Pedro J., Ramos-Mejía, Verónica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200071/
https://www.ncbi.nlm.nih.gov/pubmed/35721502
http://dx.doi.org/10.3389/fcell.2022.846092
Descripción
Sumario:Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a particular subgroup of young pediatric patients with poor outcome. We report the generation and characterization of human Embryonic Stem Cell (hESC) clonal lines with inducible expression of NK5A. Temporal control of NK5A expression during hematopoietic differentiation from hESC will be critical for elucidating its participation during the leukemogenic process.

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