Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus

Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a...

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Autores principales: Domingo-Reinés, Joan, Martínez-Navajas, Gonzalo, Montes, Rosa, Lamolda, Mar, Simón, Iris, Castaño, Julio, Ríos-Pelegrina, Rosa, Lopez-Hidalgo, Javier Luis, García del Moral, Raimundo, Marchal, Juan A., Real, Pedro J., Ramos-Mejía, Verónica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200071/
https://www.ncbi.nlm.nih.gov/pubmed/35721502
http://dx.doi.org/10.3389/fcell.2022.846092
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author Domingo-Reinés, Joan
Martínez-Navajas, Gonzalo
Montes, Rosa
Lamolda, Mar
Simón, Iris
Castaño, Julio
Ríos-Pelegrina, Rosa
Lopez-Hidalgo, Javier Luis
García del Moral, Raimundo
Marchal, Juan A.
Real, Pedro J.
Ramos-Mejía, Verónica
author_facet Domingo-Reinés, Joan
Martínez-Navajas, Gonzalo
Montes, Rosa
Lamolda, Mar
Simón, Iris
Castaño, Julio
Ríos-Pelegrina, Rosa
Lopez-Hidalgo, Javier Luis
García del Moral, Raimundo
Marchal, Juan A.
Real, Pedro J.
Ramos-Mejía, Verónica
author_sort Domingo-Reinés, Joan
collection PubMed
description Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a particular subgroup of young pediatric patients with poor outcome. We report the generation and characterization of human Embryonic Stem Cell (hESC) clonal lines with inducible expression of NK5A. Temporal control of NK5A expression during hematopoietic differentiation from hESC will be critical for elucidating its participation during the leukemogenic process.
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spelling pubmed-92000712022-06-16 Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus Domingo-Reinés, Joan Martínez-Navajas, Gonzalo Montes, Rosa Lamolda, Mar Simón, Iris Castaño, Julio Ríos-Pelegrina, Rosa Lopez-Hidalgo, Javier Luis García del Moral, Raimundo Marchal, Juan A. Real, Pedro J. Ramos-Mejía, Verónica Front Cell Dev Biol Cell and Developmental Biology Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a particular subgroup of young pediatric patients with poor outcome. We report the generation and characterization of human Embryonic Stem Cell (hESC) clonal lines with inducible expression of NK5A. Temporal control of NK5A expression during hematopoietic differentiation from hESC will be critical for elucidating its participation during the leukemogenic process. Frontiers Media S.A. 2022-06-01 /pmc/articles/PMC9200071/ /pubmed/35721502 http://dx.doi.org/10.3389/fcell.2022.846092 Text en Copyright © 2022 Domingo-Reinés, Martínez-Navajas, Montes, Lamolda, Simón, Castaño, Ríos-Pelegrina, Lopez-Hidalgo, García del Moral, Marchal, Real and Ramos-Mejía. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Domingo-Reinés, Joan
Martínez-Navajas, Gonzalo
Montes, Rosa
Lamolda, Mar
Simón, Iris
Castaño, Julio
Ríos-Pelegrina, Rosa
Lopez-Hidalgo, Javier Luis
García del Moral, Raimundo
Marchal, Juan A.
Real, Pedro J.
Ramos-Mejía, Verónica
Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus
title Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus
title_full Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus
title_fullStr Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus
title_full_unstemmed Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus
title_short Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus
title_sort generation of a h9 clonal cell line with inducible expression of nup98-kdm5a fusion gene in the aavs1 safe harbor locus
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200071/
https://www.ncbi.nlm.nih.gov/pubmed/35721502
http://dx.doi.org/10.3389/fcell.2022.846092
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