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Carnosic Acid as an intracanal medicament performs better than triple antibiotic paste and calcium hydroxide to eradicate Enterococcus faecalis from root canal: An in vitro confocal laser scanning microscopic study

BACKGROUND: Carnosic acid is an herbal derivative with potent antioxidant, anti-inflammatory, antimicrobial, and anticancer properties. AIM: Comparative evaluation of the antimicrobial potential of carnosic acid, calcium hydroxide, and triple antibiotic paste as intracanal medicaments against Entero...

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Autores principales: Dessai, Ashwini, Shetty, Neeta, Saralaya, Vishwas, Natarajan, Srikant, Mala, Kundabala
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200188/
https://www.ncbi.nlm.nih.gov/pubmed/35722077
http://dx.doi.org/10.4103/jcd.jcd_317_21
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author Dessai, Ashwini
Shetty, Neeta
Saralaya, Vishwas
Natarajan, Srikant
Mala, Kundabala
author_facet Dessai, Ashwini
Shetty, Neeta
Saralaya, Vishwas
Natarajan, Srikant
Mala, Kundabala
author_sort Dessai, Ashwini
collection PubMed
description BACKGROUND: Carnosic acid is an herbal derivative with potent antioxidant, anti-inflammatory, antimicrobial, and anticancer properties. AIM: Comparative evaluation of the antimicrobial potential of carnosic acid, calcium hydroxide, and triple antibiotic paste as intracanal medicaments against Enterococcus faecalis. SETTINGS AND DESIGN: Department of Conservative Dentistry and Microbiology, an in vitro study. MATERIALS AND METHODS: Fifty-two extracted single-rooted human teeth were decoronated and chemomechanical preparation was performed. The specimens were secured in the center of screw-capped vials and autoclaved. A strain of E. faecalis was inoculated into the canals and grown for 72 h. The teeth were divided into: Group I-Ca(OH)(2), Group II- triple antibiotic paste (TAP), Group III-Carnosic acid, and Group IV-Negative control. The medicaments were applied in the canal and left for 14 days. The specimens were sectioned transversely at three levels to create dentinal discs and observed under the confocal laser scanning microscopic (CLSM). Images were analyzed, and quantification of bacteria was done using the Image J software. STATISTICAL ANALYSIS: Mean percentage of live/dead bacteria was analyzed using One-way ANOVA and Post hoc Tukey test. RESULTS: Mean percentages of live and dead bacteria were seen under CLSM in Group I, Group II, and Group III were (4.44 ± 2.87, 4.56 ± 2.93, 1.61 ± 1.90), and (4.59 ± 3.04, 4.25 ± 2.98, 1.70 ± 1.99), respectively, with least mean percentages for live and dead bacteria in carnosic acid (Group III). CONCLUSION: Carnosic acid showed better antimicrobial efficacy against E. faecalis than TAP and Ca(OH)(2) by showing a low percentage of both live and dead bacteria.
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spelling pubmed-92001882022-06-16 Carnosic Acid as an intracanal medicament performs better than triple antibiotic paste and calcium hydroxide to eradicate Enterococcus faecalis from root canal: An in vitro confocal laser scanning microscopic study Dessai, Ashwini Shetty, Neeta Saralaya, Vishwas Natarajan, Srikant Mala, Kundabala J Conserv Dent Original Article BACKGROUND: Carnosic acid is an herbal derivative with potent antioxidant, anti-inflammatory, antimicrobial, and anticancer properties. AIM: Comparative evaluation of the antimicrobial potential of carnosic acid, calcium hydroxide, and triple antibiotic paste as intracanal medicaments against Enterococcus faecalis. SETTINGS AND DESIGN: Department of Conservative Dentistry and Microbiology, an in vitro study. MATERIALS AND METHODS: Fifty-two extracted single-rooted human teeth were decoronated and chemomechanical preparation was performed. The specimens were secured in the center of screw-capped vials and autoclaved. A strain of E. faecalis was inoculated into the canals and grown for 72 h. The teeth were divided into: Group I-Ca(OH)(2), Group II- triple antibiotic paste (TAP), Group III-Carnosic acid, and Group IV-Negative control. The medicaments were applied in the canal and left for 14 days. The specimens were sectioned transversely at three levels to create dentinal discs and observed under the confocal laser scanning microscopic (CLSM). Images were analyzed, and quantification of bacteria was done using the Image J software. STATISTICAL ANALYSIS: Mean percentage of live/dead bacteria was analyzed using One-way ANOVA and Post hoc Tukey test. RESULTS: Mean percentages of live and dead bacteria were seen under CLSM in Group I, Group II, and Group III were (4.44 ± 2.87, 4.56 ± 2.93, 1.61 ± 1.90), and (4.59 ± 3.04, 4.25 ± 2.98, 1.70 ± 1.99), respectively, with least mean percentages for live and dead bacteria in carnosic acid (Group III). CONCLUSION: Carnosic acid showed better antimicrobial efficacy against E. faecalis than TAP and Ca(OH)(2) by showing a low percentage of both live and dead bacteria. Wolters Kluwer - Medknow 2022 2022-05-02 /pmc/articles/PMC9200188/ /pubmed/35722077 http://dx.doi.org/10.4103/jcd.jcd_317_21 Text en Copyright: © 2022 Journal of Conservative Dentistry https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Dessai, Ashwini
Shetty, Neeta
Saralaya, Vishwas
Natarajan, Srikant
Mala, Kundabala
Carnosic Acid as an intracanal medicament performs better than triple antibiotic paste and calcium hydroxide to eradicate Enterococcus faecalis from root canal: An in vitro confocal laser scanning microscopic study
title Carnosic Acid as an intracanal medicament performs better than triple antibiotic paste and calcium hydroxide to eradicate Enterococcus faecalis from root canal: An in vitro confocal laser scanning microscopic study
title_full Carnosic Acid as an intracanal medicament performs better than triple antibiotic paste and calcium hydroxide to eradicate Enterococcus faecalis from root canal: An in vitro confocal laser scanning microscopic study
title_fullStr Carnosic Acid as an intracanal medicament performs better than triple antibiotic paste and calcium hydroxide to eradicate Enterococcus faecalis from root canal: An in vitro confocal laser scanning microscopic study
title_full_unstemmed Carnosic Acid as an intracanal medicament performs better than triple antibiotic paste and calcium hydroxide to eradicate Enterococcus faecalis from root canal: An in vitro confocal laser scanning microscopic study
title_short Carnosic Acid as an intracanal medicament performs better than triple antibiotic paste and calcium hydroxide to eradicate Enterococcus faecalis from root canal: An in vitro confocal laser scanning microscopic study
title_sort carnosic acid as an intracanal medicament performs better than triple antibiotic paste and calcium hydroxide to eradicate enterococcus faecalis from root canal: an in vitro confocal laser scanning microscopic study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200188/
https://www.ncbi.nlm.nih.gov/pubmed/35722077
http://dx.doi.org/10.4103/jcd.jcd_317_21
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