Optimization of BRET saturation assays for robust and sensitive cytosolic protein–protein interaction studies

Bioluminescence resonance energy transfer (BRET) saturation is a method of studying protein–protein interaction (PPI) upon quantification of the dependence of the BRET signal on the acceptor/donor (A:D) expression ratio. In this study, using the very bright Nluc/YFP BRET pair acquired respectively w...

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Detalles Bibliográficos
Autores principales: Besson, Benoit, Eun, Hyeju, Kim, Seonhee, Windisch, Marc P., Bourhy, Herve, Grailhe, Regis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200754/
https://www.ncbi.nlm.nih.gov/pubmed/35705637
http://dx.doi.org/10.1038/s41598-022-12851-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200754/
https://www.ncbi.nlm.nih.gov/pubmed/35705637
http://dx.doi.org/10.1038/s41598-022-12851-9

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