Reengineering the specificity of the highly selective Clostridium botulinum protease via directed evolution

The botulinum neurotoxin serotype A (BoNT/A) cuts a single peptide bond in SNAP25, an activity used to treat a wide range of diseases. Reengineering the substrate specificity of BoNT/A’s protease domain (LC/A) could expand its therapeutic applications; however, LC/A’s extended substrate recognition...

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Detalles Bibliográficos
Autores principales: Dyer, Rebekah P., Isoda, Hariny M., Salcedo, Gabriela S., Speciale, Gaetano, Fletcher, Madison H., Le, Linh Q., Liu, Yi, Brami-Cherrier, Karen, Malik, Shiazah Z., Vazquez-Cintron, Edwin J., Chu, Andrew C., Rupp, David C., Jacky, Birgitte P. S., Nguyen, Thu T. M., Katz, Benjamin B., Steward, Lance E., Majumdar, Sudipta, Brideau-Andersen, Amy D., Weiss, Gregory A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200782/
https://www.ncbi.nlm.nih.gov/pubmed/35705606
http://dx.doi.org/10.1038/s41598-022-13617-z

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