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Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology
Standardised and high-throughput methods have been developed for the production and experimental handling of some 3D in vitro models. However, adapted analytical tools are still missing for scientists and researchers to fully exploit the potential of complex cellular models in pre-clinical drug test...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200833/ https://www.ncbi.nlm.nih.gov/pubmed/35705590 http://dx.doi.org/10.1038/s41598-022-13987-4 |
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author | Heub, Sarah Navaee, Fatemeh Migliozzi, Daniel Ledroit, Diane Boder-Pasche, Stéphanie Goldowsky, Jonas Vuille-Dit-Bille, Emilie Hofer, Joëlle Gaiser, Carine Revol, Vincent Suter-Dick, Laura Weder, Gilles |
author_facet | Heub, Sarah Navaee, Fatemeh Migliozzi, Daniel Ledroit, Diane Boder-Pasche, Stéphanie Goldowsky, Jonas Vuille-Dit-Bille, Emilie Hofer, Joëlle Gaiser, Carine Revol, Vincent Suter-Dick, Laura Weder, Gilles |
author_sort | Heub, Sarah |
collection | PubMed |
description | Standardised and high-throughput methods have been developed for the production and experimental handling of some 3D in vitro models. However, adapted analytical tools are still missing for scientists and researchers to fully exploit the potential of complex cellular models in pre-clinical drug testing and precision medicine. Histology is the established, cost-effective and gold standard method for structural and functional tissue analysis. However, standard histological processes are challenging and costly to apply to 3D cell models, as their small size often leads to poor alignment of samples, which lowers analysis throughput. This body of work proposes a new approach: HistoBrick facilitates histological processing of spheroids and organoids by enabling gel embedding of 3D cell models with precise coplanar alignment, parallel to the sectioning plane, thus minimising the loss of sample material. HistoBrick’s features are compatible with automation standards, potentially allowing automated sample transfer from a multi-well plate to the gel device. Moreover, HistoBrick’s technology was validated by demonstrating the alignment of HepG2 cultured spheroids measuring 150–200 µm in diameter with a height precision of ± 80 µm. HistoBrick allows up to 96 samples to be studied across minimal sections, paving the way towards high-throughput micro-histology. |
format | Online Article Text |
id | pubmed-9200833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-92008332022-06-17 Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology Heub, Sarah Navaee, Fatemeh Migliozzi, Daniel Ledroit, Diane Boder-Pasche, Stéphanie Goldowsky, Jonas Vuille-Dit-Bille, Emilie Hofer, Joëlle Gaiser, Carine Revol, Vincent Suter-Dick, Laura Weder, Gilles Sci Rep Article Standardised and high-throughput methods have been developed for the production and experimental handling of some 3D in vitro models. However, adapted analytical tools are still missing for scientists and researchers to fully exploit the potential of complex cellular models in pre-clinical drug testing and precision medicine. Histology is the established, cost-effective and gold standard method for structural and functional tissue analysis. However, standard histological processes are challenging and costly to apply to 3D cell models, as their small size often leads to poor alignment of samples, which lowers analysis throughput. This body of work proposes a new approach: HistoBrick facilitates histological processing of spheroids and organoids by enabling gel embedding of 3D cell models with precise coplanar alignment, parallel to the sectioning plane, thus minimising the loss of sample material. HistoBrick’s features are compatible with automation standards, potentially allowing automated sample transfer from a multi-well plate to the gel device. Moreover, HistoBrick’s technology was validated by demonstrating the alignment of HepG2 cultured spheroids measuring 150–200 µm in diameter with a height precision of ± 80 µm. HistoBrick allows up to 96 samples to be studied across minimal sections, paving the way towards high-throughput micro-histology. Nature Publishing Group UK 2022-06-15 /pmc/articles/PMC9200833/ /pubmed/35705590 http://dx.doi.org/10.1038/s41598-022-13987-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Heub, Sarah Navaee, Fatemeh Migliozzi, Daniel Ledroit, Diane Boder-Pasche, Stéphanie Goldowsky, Jonas Vuille-Dit-Bille, Emilie Hofer, Joëlle Gaiser, Carine Revol, Vincent Suter-Dick, Laura Weder, Gilles Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology |
title | Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology |
title_full | Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology |
title_fullStr | Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology |
title_full_unstemmed | Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology |
title_short | Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology |
title_sort | coplanar embedding of multiple 3d cell models in hydrogel towards high-throughput micro-histology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200833/ https://www.ncbi.nlm.nih.gov/pubmed/35705590 http://dx.doi.org/10.1038/s41598-022-13987-4 |
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