Cargando…
Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR‐383‐5p and regulating KIAA0101
BACKGROUND: Circular RNAs (circRNAs) can act as key regulators in human cancers, including esophageal squamous cell carcinoma (ESCC). However, the role and mechanism of circ_0005231 in ESCC have not previously been reported. METHODS: RNA levels and protein levels were detected by real‐time quantitat...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons Australia, Ltd
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200875/ https://www.ncbi.nlm.nih.gov/pubmed/35524161 http://dx.doi.org/10.1111/1759-7714.14450 |
_version_ | 1784728163828367360 |
---|---|
author | Sun, Zhiguang Zhang, Shaowei Zhang, Nan Wang, Jianxin Wang, Jindong Liu, Junfeng |
author_facet | Sun, Zhiguang Zhang, Shaowei Zhang, Nan Wang, Jianxin Wang, Jindong Liu, Junfeng |
author_sort | Sun, Zhiguang |
collection | PubMed |
description | BACKGROUND: Circular RNAs (circRNAs) can act as key regulators in human cancers, including esophageal squamous cell carcinoma (ESCC). However, the role and mechanism of circ_0005231 in ESCC have not previously been reported. METHODS: RNA levels and protein levels were detected by real‐time quantitative polymerase chain reaction (RT‐qPCR) and Western blot assay, respectively. Cell proliferation was assessed by colony formation assay and 5‐ethynyl‐2'‐deoxyuridine (EdU) assay. Wound healing and transwell assays were used to assess cell migration and invasion, respectively. The intermolecular interaction was predicted by bioinformatic analysis and verified by RNA immunoprecipitation (RIP), RNA pulldown and dual‐luciferase reporter assays. Xenograft tumor model was used for exploring the biological function of circ_0005231 in vivo. RESULTS: Circ_0005231 was upregulated in ESCC plasma, tissues and cells. Cell proliferation, migration and invasion were significantly restrained by knockdown of circ_0005231 in ESCC cells. Circ_0005231 acted as a sponge of miR‐383‐5p, and circ_0005231 regulated ESCC cellular behavior by sponging miR‐383‐5p. Moreover, miR‐383‐5p directly targeted KIAA0101, and circ_0005231 positively regulated KIAA0101 expression by sponging miR‐383‐5p. Furthermore, circ_0005231 knockdown suppressed the malignant behavior of ESCC cells by downregulating KIAA0101. Importantly, knockdown of circ_0005231 blocked xenograft tumor growth in vivo. CONCLUSION: Circ_0005231 acted as a sponge of miR‐383‐5p to promote ESCC progression by upregulating KIAA0101, which provided a potential therapeutic strategy for ESCC treatment. |
format | Online Article Text |
id | pubmed-9200875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley & Sons Australia, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-92008752022-06-23 Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR‐383‐5p and regulating KIAA0101 Sun, Zhiguang Zhang, Shaowei Zhang, Nan Wang, Jianxin Wang, Jindong Liu, Junfeng Thorac Cancer Original Articles BACKGROUND: Circular RNAs (circRNAs) can act as key regulators in human cancers, including esophageal squamous cell carcinoma (ESCC). However, the role and mechanism of circ_0005231 in ESCC have not previously been reported. METHODS: RNA levels and protein levels were detected by real‐time quantitative polymerase chain reaction (RT‐qPCR) and Western blot assay, respectively. Cell proliferation was assessed by colony formation assay and 5‐ethynyl‐2'‐deoxyuridine (EdU) assay. Wound healing and transwell assays were used to assess cell migration and invasion, respectively. The intermolecular interaction was predicted by bioinformatic analysis and verified by RNA immunoprecipitation (RIP), RNA pulldown and dual‐luciferase reporter assays. Xenograft tumor model was used for exploring the biological function of circ_0005231 in vivo. RESULTS: Circ_0005231 was upregulated in ESCC plasma, tissues and cells. Cell proliferation, migration and invasion were significantly restrained by knockdown of circ_0005231 in ESCC cells. Circ_0005231 acted as a sponge of miR‐383‐5p, and circ_0005231 regulated ESCC cellular behavior by sponging miR‐383‐5p. Moreover, miR‐383‐5p directly targeted KIAA0101, and circ_0005231 positively regulated KIAA0101 expression by sponging miR‐383‐5p. Furthermore, circ_0005231 knockdown suppressed the malignant behavior of ESCC cells by downregulating KIAA0101. Importantly, knockdown of circ_0005231 blocked xenograft tumor growth in vivo. CONCLUSION: Circ_0005231 acted as a sponge of miR‐383‐5p to promote ESCC progression by upregulating KIAA0101, which provided a potential therapeutic strategy for ESCC treatment. John Wiley & Sons Australia, Ltd 2022-05-06 2022-06 /pmc/articles/PMC9200875/ /pubmed/35524161 http://dx.doi.org/10.1111/1759-7714.14450 Text en © 2022 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Sun, Zhiguang Zhang, Shaowei Zhang, Nan Wang, Jianxin Wang, Jindong Liu, Junfeng Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR‐383‐5p and regulating KIAA0101 |
title | Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR‐383‐5p and regulating KIAA0101
|
title_full | Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR‐383‐5p and regulating KIAA0101
|
title_fullStr | Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR‐383‐5p and regulating KIAA0101
|
title_full_unstemmed | Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR‐383‐5p and regulating KIAA0101
|
title_short | Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR‐383‐5p and regulating KIAA0101
|
title_sort | circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging mir‐383‐5p and regulating kiaa0101 |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200875/ https://www.ncbi.nlm.nih.gov/pubmed/35524161 http://dx.doi.org/10.1111/1759-7714.14450 |
work_keys_str_mv | AT sunzhiguang circ0005231promotestheprogressionofesophagealsquamouscellcarcinomaviaspongingmir3835pandregulatingkiaa0101 AT zhangshaowei circ0005231promotestheprogressionofesophagealsquamouscellcarcinomaviaspongingmir3835pandregulatingkiaa0101 AT zhangnan circ0005231promotestheprogressionofesophagealsquamouscellcarcinomaviaspongingmir3835pandregulatingkiaa0101 AT wangjianxin circ0005231promotestheprogressionofesophagealsquamouscellcarcinomaviaspongingmir3835pandregulatingkiaa0101 AT wangjindong circ0005231promotestheprogressionofesophagealsquamouscellcarcinomaviaspongingmir3835pandregulatingkiaa0101 AT liujunfeng circ0005231promotestheprogressionofesophagealsquamouscellcarcinomaviaspongingmir3835pandregulatingkiaa0101 |