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Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance

The F-box gene family is one of the largest gene families in plants. These genes regulate plant growth and development, as well as biotic and abiotic stress responses, and they have been extensively researched. Drought stress is one of the major factors limiting the yield and quality of soybean. In...

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Autores principales: Xu, Keheng, Zhao, Yu, Zhao, Yan, Feng, Chen, Zhang, Yinhe, Wang, Fawei, Li, Xiaowei, Gao, Hongtao, Liu, Weican, Jing, Yan, Saxena, Rachit K., Feng, Xianzhong, Zhou, Yonggang, Li, Haiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201338/
https://www.ncbi.nlm.nih.gov/pubmed/35720533
http://dx.doi.org/10.3389/fpls.2022.823529
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author Xu, Keheng
Zhao, Yu
Zhao, Yan
Feng, Chen
Zhang, Yinhe
Wang, Fawei
Li, Xiaowei
Gao, Hongtao
Liu, Weican
Jing, Yan
Saxena, Rachit K.
Feng, Xianzhong
Zhou, Yonggang
Li, Haiyan
author_facet Xu, Keheng
Zhao, Yu
Zhao, Yan
Feng, Chen
Zhang, Yinhe
Wang, Fawei
Li, Xiaowei
Gao, Hongtao
Liu, Weican
Jing, Yan
Saxena, Rachit K.
Feng, Xianzhong
Zhou, Yonggang
Li, Haiyan
author_sort Xu, Keheng
collection PubMed
description The F-box gene family is one of the largest gene families in plants. These genes regulate plant growth and development, as well as biotic and abiotic stress responses, and they have been extensively researched. Drought stress is one of the major factors limiting the yield and quality of soybean. In this study, bioinformatics analysis of the soybean F-box gene family was performed, and the role of soybean F-box-like gene GmFBL144 in drought stress adaptation was characterized. We identified 507 F-box genes in the soybean genome database, which were classified into 11 subfamilies. The expression profiles showed that GmFBL144 was highly expressed in plant roots. Overexpression of GmFBL144 increased the sensitivity of transgenic Arabidopsis to drought stress. Under drought stress, the hydrogen peroxide (H(2)O(2)) and malonaldehyde (MDA) contents of transgenic Arabidopsis were higher than those of the wild type (WT) and empty vector control, and the chlorophyll content was lower than that of the control. Y2H and bimolecular fluorescence complementation (BiFC) assays showed that GmFBL144 can interact with GmsHSP. Furthermore, our results showed that GmFBL144 can form SCF(FBL144) (E3 ubiquitin ligase) with GmSkp1 and GmCullin1. Altogether, these results indicate that the soybean F-box-like protein GmFBL144 may negatively regulate plant drought stress tolerance by interacting with sHSP. These findings provide a basis for molecular genetics and breeding of soybean.
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spelling pubmed-92013382022-06-17 Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance Xu, Keheng Zhao, Yu Zhao, Yan Feng, Chen Zhang, Yinhe Wang, Fawei Li, Xiaowei Gao, Hongtao Liu, Weican Jing, Yan Saxena, Rachit K. Feng, Xianzhong Zhou, Yonggang Li, Haiyan Front Plant Sci Plant Science The F-box gene family is one of the largest gene families in plants. These genes regulate plant growth and development, as well as biotic and abiotic stress responses, and they have been extensively researched. Drought stress is one of the major factors limiting the yield and quality of soybean. In this study, bioinformatics analysis of the soybean F-box gene family was performed, and the role of soybean F-box-like gene GmFBL144 in drought stress adaptation was characterized. We identified 507 F-box genes in the soybean genome database, which were classified into 11 subfamilies. The expression profiles showed that GmFBL144 was highly expressed in plant roots. Overexpression of GmFBL144 increased the sensitivity of transgenic Arabidopsis to drought stress. Under drought stress, the hydrogen peroxide (H(2)O(2)) and malonaldehyde (MDA) contents of transgenic Arabidopsis were higher than those of the wild type (WT) and empty vector control, and the chlorophyll content was lower than that of the control. Y2H and bimolecular fluorescence complementation (BiFC) assays showed that GmFBL144 can interact with GmsHSP. Furthermore, our results showed that GmFBL144 can form SCF(FBL144) (E3 ubiquitin ligase) with GmSkp1 and GmCullin1. Altogether, these results indicate that the soybean F-box-like protein GmFBL144 may negatively regulate plant drought stress tolerance by interacting with sHSP. These findings provide a basis for molecular genetics and breeding of soybean. Frontiers Media S.A. 2022-06-02 /pmc/articles/PMC9201338/ /pubmed/35720533 http://dx.doi.org/10.3389/fpls.2022.823529 Text en Copyright © 2022 Xu, Zhao, Zhao, Feng, Zhang, Wang, Li, Gao, Liu, Jing, Saxena, Feng, Zhou and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xu, Keheng
Zhao, Yu
Zhao, Yan
Feng, Chen
Zhang, Yinhe
Wang, Fawei
Li, Xiaowei
Gao, Hongtao
Liu, Weican
Jing, Yan
Saxena, Rachit K.
Feng, Xianzhong
Zhou, Yonggang
Li, Haiyan
Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance
title Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance
title_full Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance
title_fullStr Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance
title_full_unstemmed Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance
title_short Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance
title_sort soybean f-box-like protein gmfbl144 interacts with small heat shock protein and negatively regulates plant drought stress tolerance
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201338/
https://www.ncbi.nlm.nih.gov/pubmed/35720533
http://dx.doi.org/10.3389/fpls.2022.823529
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