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The Cellular and Viral circRNAs Induced by Fowl Adenovirus Serotype 4 Infection

Circular RNAs (circRNAs) are a new class of noncoding RNAs that play vital roles in many biological processes. Virus infection induces modifications in cellular circRNA transcriptomes and expresses viral circRNAs. The outbreaks of Hydropericardium-hepatitis syndrome (HHS) caused by fowl adenovirus s...

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Autores principales: Liu, Xiao-Na, Guo, Xiao-Ran, Han, Ying, Tian, Tian, Sun, Jian, Lei, Bai-Shi, Zhang, Wu-Chao, Yuan, Wan-Zhe, Zhao, Kuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201442/
https://www.ncbi.nlm.nih.gov/pubmed/35722302
http://dx.doi.org/10.3389/fmicb.2022.925953
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author Liu, Xiao-Na
Guo, Xiao-Ran
Han, Ying
Tian, Tian
Sun, Jian
Lei, Bai-Shi
Zhang, Wu-Chao
Yuan, Wan-Zhe
Zhao, Kuan
author_facet Liu, Xiao-Na
Guo, Xiao-Ran
Han, Ying
Tian, Tian
Sun, Jian
Lei, Bai-Shi
Zhang, Wu-Chao
Yuan, Wan-Zhe
Zhao, Kuan
author_sort Liu, Xiao-Na
collection PubMed
description Circular RNAs (circRNAs) are a new class of noncoding RNAs that play vital roles in many biological processes. Virus infection induces modifications in cellular circRNA transcriptomes and expresses viral circRNAs. The outbreaks of Hydropericardium-hepatitis syndrome (HHS) caused by fowl adenovirus serotype 4 (FAdV-4) have resulted in huge economic losses to the poultry industry worldwide. To investigate the expression of circRNAs during FAdV-4 infection, we performed transcriptome analysis of FAdV-4-infected leghorn male hepatoma (LMH) cells. In total, 19,154 cellular circRNAs and 135 differentially expressed (DE) cellular circRNAs were identified. The characteristics of the DE cellular circRNAs were analyzed and most of them were related to multiple biological processes according to GO and KEGG enrichment analysis. The accuracy of 10 cellular circRNAs were verified by semiquantitative RT-PCR and sequencing. The change trend was consistent with the RNA sequencing results. Moreover, 2014 viral circRNAs were identified and 10 circRNAs were verified by the same methods. Our analysis showed that seven circRNAs with the same 3′ terminal and variable 5′ terminal regions were located at pTP protein and DNA pol protein of FAdV-4, which may be generated via alternative splicing events. Moreover, the expression level of viral circRNAs was closely related to the replication efficiency of the virus and partial of the viral circRNAs promoted the replication of FAdV-4. Competing endogenous RNA analysis further showed that the effects of cellular and viral circRNAs on host or viral genes may act via miRNAs. Collectively, our findings first indicate that FAdV-4 infection induced the differential expression of cellular circRNAs and FAdV-4 also expressed viral circRNAs, some of which affected FAdV-4 replication. These findings will provide new clues for further understanding FAdV-4 and provide a basis for investigating host-virus interactions.
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spelling pubmed-92014422022-06-17 The Cellular and Viral circRNAs Induced by Fowl Adenovirus Serotype 4 Infection Liu, Xiao-Na Guo, Xiao-Ran Han, Ying Tian, Tian Sun, Jian Lei, Bai-Shi Zhang, Wu-Chao Yuan, Wan-Zhe Zhao, Kuan Front Microbiol Microbiology Circular RNAs (circRNAs) are a new class of noncoding RNAs that play vital roles in many biological processes. Virus infection induces modifications in cellular circRNA transcriptomes and expresses viral circRNAs. The outbreaks of Hydropericardium-hepatitis syndrome (HHS) caused by fowl adenovirus serotype 4 (FAdV-4) have resulted in huge economic losses to the poultry industry worldwide. To investigate the expression of circRNAs during FAdV-4 infection, we performed transcriptome analysis of FAdV-4-infected leghorn male hepatoma (LMH) cells. In total, 19,154 cellular circRNAs and 135 differentially expressed (DE) cellular circRNAs were identified. The characteristics of the DE cellular circRNAs were analyzed and most of them were related to multiple biological processes according to GO and KEGG enrichment analysis. The accuracy of 10 cellular circRNAs were verified by semiquantitative RT-PCR and sequencing. The change trend was consistent with the RNA sequencing results. Moreover, 2014 viral circRNAs were identified and 10 circRNAs were verified by the same methods. Our analysis showed that seven circRNAs with the same 3′ terminal and variable 5′ terminal regions were located at pTP protein and DNA pol protein of FAdV-4, which may be generated via alternative splicing events. Moreover, the expression level of viral circRNAs was closely related to the replication efficiency of the virus and partial of the viral circRNAs promoted the replication of FAdV-4. Competing endogenous RNA analysis further showed that the effects of cellular and viral circRNAs on host or viral genes may act via miRNAs. Collectively, our findings first indicate that FAdV-4 infection induced the differential expression of cellular circRNAs and FAdV-4 also expressed viral circRNAs, some of which affected FAdV-4 replication. These findings will provide new clues for further understanding FAdV-4 and provide a basis for investigating host-virus interactions. Frontiers Media S.A. 2022-06-02 /pmc/articles/PMC9201442/ /pubmed/35722302 http://dx.doi.org/10.3389/fmicb.2022.925953 Text en Copyright © 2022 Liu, Guo, Han, Tian, Sun, Lei, Zhang, Yuan and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Xiao-Na
Guo, Xiao-Ran
Han, Ying
Tian, Tian
Sun, Jian
Lei, Bai-Shi
Zhang, Wu-Chao
Yuan, Wan-Zhe
Zhao, Kuan
The Cellular and Viral circRNAs Induced by Fowl Adenovirus Serotype 4 Infection
title The Cellular and Viral circRNAs Induced by Fowl Adenovirus Serotype 4 Infection
title_full The Cellular and Viral circRNAs Induced by Fowl Adenovirus Serotype 4 Infection
title_fullStr The Cellular and Viral circRNAs Induced by Fowl Adenovirus Serotype 4 Infection
title_full_unstemmed The Cellular and Viral circRNAs Induced by Fowl Adenovirus Serotype 4 Infection
title_short The Cellular and Viral circRNAs Induced by Fowl Adenovirus Serotype 4 Infection
title_sort cellular and viral circrnas induced by fowl adenovirus serotype 4 infection
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201442/
https://www.ncbi.nlm.nih.gov/pubmed/35722302
http://dx.doi.org/10.3389/fmicb.2022.925953
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