Effects of Dark Septate Endophytes on the Performance and Soil Microbia of Lycium ruthenicum Under Drought Stress

In the current study, we explored the effects of dark septate endophytes (DSE) (Neocamarosporium phragmitis, Alternaria chlamydospore, and Microascus alveolaris) on the performance and rhizosphere soil microbial composition of Lycium ruthenicum Murr under drought stress. Differences in plant growth and physiological indexes, soil parameters, and microbial composition under different treatments were studied. Three DSE species could form good symbiotic relationships with L. ruthenicum plants, and the symbionts depended on DSE species and water availability. Inoculation of DSE had the greatest benefit on host plants under drought conditions. In particular, N. phragmitis and A. chlamydospore had a significant positive influence on the biomass, morphological and physiological indexes of host plants. Additionally, the content of arbuscular mycorrhiza (AM) fungi, gram-negative bacteria, and actinomycetes in the soil was significantly elevated after DSE inoculation in the absence of water. Based on a variance decomposition analysis, DSE was the most important factor affecting the growth and physiological parameters of host plants, and DSE inoculation combined with water conditions significantly affected the contents of soil microbial communities. Structural equation model (SEM) analysis showed that the positive effects of DSE on L. ruthenicum varied with DSE species and plant parameters under different water conditions. These results are helpful to understand the ecological function of DSE and its potential application in the cultivation of L. ruthenicum plants in drylands.