Bipolar Electrode Arrays for Chemical Imaging and Multiplexed Sensing

[Image: see text] Bipolar electrodes (BPEs) with arrays of cathodic and anodic poles were developed for use in closed bipolar systems. To increase the number of BPEs in the array, the anodic and cathodic poles were connected with each other using thin leads. A further increase in the number of BPEs was achieved by forming the cathodic and anodic poles of the BPEs and the leads in different layers. A device with 9 × 10 arrays of cathodes and anodes was thus realized. When using this device to sense hydrogen peroxide (H(2)O(2)), the sensitivity and linear range of calibration plots could be adjusted by changing the driving voltage and the area ratio between the cathodic and anodic poles. The devices were used to image H(2)O(2) and obtain time-lapse images for the diffusion and dilution of H(2)O(2). Furthermore, DNA detection was demonstrated using an electroactive intercalator. The sensitivity could be improved by making the anodic poles smaller with respect to the cathodic pole and concentrating the electrochemiluminescence (ECL) in a small area. The ECL intensity changed according to the target DNA concentration in the solution.