Cargando…

Changes in Systemic Regulatory T Cells, Effector T Cells, and Monocyte Populations Associated With Early-Life Stunting

Stunting and environmental enteric dysfunction (EED) may be responsible for altered gut and systemic immune responses. However, their impact on circulating immune cell populations remains poorly characterized during early life. A detailed flow cytometry analysis of major systemic immune cell populat...

Descripción completa

Detalles Bibliográficos
Autores principales: Andriamanantena, Zo, Randrianarisaona, Fanirisoa, Rakotondrainipiana, Maheninasy, Andriantsalama, Prisca, Randriamparany, Ravaka, Randremanana, Rindra, Randrianirina, Frédérique, Novault, Sophie, Duffy, Darragh, Huetz, François, Hasan, Milena, Schoenhals, Matthieu, Sansonetti, Philippe J., Vonaesch, Pascale, Vigan-Womas, Inès
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202423/
https://www.ncbi.nlm.nih.gov/pubmed/35720335
http://dx.doi.org/10.3389/fimmu.2022.864084
_version_ 1784728529553850368
author Andriamanantena, Zo
Randrianarisaona, Fanirisoa
Rakotondrainipiana, Maheninasy
Andriantsalama, Prisca
Randriamparany, Ravaka
Randremanana, Rindra
Randrianirina, Frédérique
Novault, Sophie
Duffy, Darragh
Huetz, François
Hasan, Milena
Schoenhals, Matthieu
Sansonetti, Philippe J.
Vonaesch, Pascale
Vigan-Womas, Inès
author_facet Andriamanantena, Zo
Randrianarisaona, Fanirisoa
Rakotondrainipiana, Maheninasy
Andriantsalama, Prisca
Randriamparany, Ravaka
Randremanana, Rindra
Randrianirina, Frédérique
Novault, Sophie
Duffy, Darragh
Huetz, François
Hasan, Milena
Schoenhals, Matthieu
Sansonetti, Philippe J.
Vonaesch, Pascale
Vigan-Womas, Inès
author_sort Andriamanantena, Zo
collection PubMed
description Stunting and environmental enteric dysfunction (EED) may be responsible for altered gut and systemic immune responses. However, their impact on circulating immune cell populations remains poorly characterized during early life. A detailed flow cytometry analysis of major systemic immune cell populations in 53 stunted and 52 non-stunted (2 to 5 years old) children living in Antananarivo (Madagascar) was performed. Compared to age-matched non-stunted controls, stunted children aged 2-3 years old had a significantly lower relative proportion of classical monocytes. No significant associations were found between stunting and the percentages of effector T helper cell populations (Th1, Th2, Th17, Th1Th17, and cTfh). However, we found that HLA-DR expression (MFI) on all memory CD4(+) or CD8(+) T cell subsets was significantly lower in stunted children compared to non-stunted controls. Interestingly, in stunted children compared to the same age-matched non-stunted controls, we observed statistically significant age-specific differences in regulatory T cells (Treg) subsets. Indeed, in 2- to 3-year-old stunted children, a significantly higher percentage of memory Treg, whilst a significantly lower percentage of naive Treg, was found. Our results revealed that both innate and adaptive systemic cell percentages, as well as activation status, were impacted in an age-related manner during stunting. Our study provides valuable insights into the understanding of systemic immune system changes in stunted children.
format Online
Article
Text
id pubmed-9202423
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92024232022-06-17 Changes in Systemic Regulatory T Cells, Effector T Cells, and Monocyte Populations Associated With Early-Life Stunting Andriamanantena, Zo Randrianarisaona, Fanirisoa Rakotondrainipiana, Maheninasy Andriantsalama, Prisca Randriamparany, Ravaka Randremanana, Rindra Randrianirina, Frédérique Novault, Sophie Duffy, Darragh Huetz, François Hasan, Milena Schoenhals, Matthieu Sansonetti, Philippe J. Vonaesch, Pascale Vigan-Womas, Inès Front Immunol Immunology Stunting and environmental enteric dysfunction (EED) may be responsible for altered gut and systemic immune responses. However, their impact on circulating immune cell populations remains poorly characterized during early life. A detailed flow cytometry analysis of major systemic immune cell populations in 53 stunted and 52 non-stunted (2 to 5 years old) children living in Antananarivo (Madagascar) was performed. Compared to age-matched non-stunted controls, stunted children aged 2-3 years old had a significantly lower relative proportion of classical monocytes. No significant associations were found between stunting and the percentages of effector T helper cell populations (Th1, Th2, Th17, Th1Th17, and cTfh). However, we found that HLA-DR expression (MFI) on all memory CD4(+) or CD8(+) T cell subsets was significantly lower in stunted children compared to non-stunted controls. Interestingly, in stunted children compared to the same age-matched non-stunted controls, we observed statistically significant age-specific differences in regulatory T cells (Treg) subsets. Indeed, in 2- to 3-year-old stunted children, a significantly higher percentage of memory Treg, whilst a significantly lower percentage of naive Treg, was found. Our results revealed that both innate and adaptive systemic cell percentages, as well as activation status, were impacted in an age-related manner during stunting. Our study provides valuable insights into the understanding of systemic immune system changes in stunted children. Frontiers Media S.A. 2022-06-02 /pmc/articles/PMC9202423/ /pubmed/35720335 http://dx.doi.org/10.3389/fimmu.2022.864084 Text en Copyright © 2022 Andriamanantena, Randrianarisaona, Rakotondrainipiana, Andriantsalama, Randriamparany, Randremanana, Randrianirina, Novault, Duffy, Huetz, Hasan, Schoenhals, Sansonetti, Vonaesch, Vigan-Womas and Afribiota Investigators https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Andriamanantena, Zo
Randrianarisaona, Fanirisoa
Rakotondrainipiana, Maheninasy
Andriantsalama, Prisca
Randriamparany, Ravaka
Randremanana, Rindra
Randrianirina, Frédérique
Novault, Sophie
Duffy, Darragh
Huetz, François
Hasan, Milena
Schoenhals, Matthieu
Sansonetti, Philippe J.
Vonaesch, Pascale
Vigan-Womas, Inès
Changes in Systemic Regulatory T Cells, Effector T Cells, and Monocyte Populations Associated With Early-Life Stunting
title Changes in Systemic Regulatory T Cells, Effector T Cells, and Monocyte Populations Associated With Early-Life Stunting
title_full Changes in Systemic Regulatory T Cells, Effector T Cells, and Monocyte Populations Associated With Early-Life Stunting
title_fullStr Changes in Systemic Regulatory T Cells, Effector T Cells, and Monocyte Populations Associated With Early-Life Stunting
title_full_unstemmed Changes in Systemic Regulatory T Cells, Effector T Cells, and Monocyte Populations Associated With Early-Life Stunting
title_short Changes in Systemic Regulatory T Cells, Effector T Cells, and Monocyte Populations Associated With Early-Life Stunting
title_sort changes in systemic regulatory t cells, effector t cells, and monocyte populations associated with early-life stunting
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202423/
https://www.ncbi.nlm.nih.gov/pubmed/35720335
http://dx.doi.org/10.3389/fimmu.2022.864084
work_keys_str_mv AT andriamanantenazo changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT randrianarisaonafanirisoa changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT rakotondrainipianamaheninasy changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT andriantsalamaprisca changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT randriamparanyravaka changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT randremananarindra changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT randrianirinafrederique changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT novaultsophie changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT duffydarragh changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT huetzfrancois changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT hasanmilena changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT schoenhalsmatthieu changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT sansonettiphilippej changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT vonaeschpascale changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT viganwomasines changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting
AT changesinsystemicregulatorytcellseffectortcellsandmonocytepopulationsassociatedwithearlylifestunting