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Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring

[Image: see text] The ability to continuously monitor the concentration of specific molecules in the body is a long-sought goal of biomedical research. For this purpose, interstitial fluid (ISF) was proposed as the ideal target biofluid because its composition can rapidly equilibrate with that of sy...

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Autores principales: Wu, Yao, Tehrani, Farshad, Teymourian, Hazhir, Mack, John, Shaver, Alexander, Reynoso, Maria, Kavner, Jonathan, Huang, Nickey, Furmidge, Allison, Duvvuri, Andrés, Nie, Yuhang, Laffel, Lori M., Doyle, Francis J., Patti, Mary-Elizabeth, Dassau, Eyal, Wang, Joseph, Arroyo-Currás, Netzahualcóyotl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202557/
https://www.ncbi.nlm.nih.gov/pubmed/35653647
http://dx.doi.org/10.1021/acs.analchem.2c00829
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author Wu, Yao
Tehrani, Farshad
Teymourian, Hazhir
Mack, John
Shaver, Alexander
Reynoso, Maria
Kavner, Jonathan
Huang, Nickey
Furmidge, Allison
Duvvuri, Andrés
Nie, Yuhang
Laffel, Lori M.
Doyle, Francis J.
Patti, Mary-Elizabeth
Dassau, Eyal
Wang, Joseph
Arroyo-Currás, Netzahualcóyotl
author_facet Wu, Yao
Tehrani, Farshad
Teymourian, Hazhir
Mack, John
Shaver, Alexander
Reynoso, Maria
Kavner, Jonathan
Huang, Nickey
Furmidge, Allison
Duvvuri, Andrés
Nie, Yuhang
Laffel, Lori M.
Doyle, Francis J.
Patti, Mary-Elizabeth
Dassau, Eyal
Wang, Joseph
Arroyo-Currás, Netzahualcóyotl
author_sort Wu, Yao
collection PubMed
description [Image: see text] The ability to continuously monitor the concentration of specific molecules in the body is a long-sought goal of biomedical research. For this purpose, interstitial fluid (ISF) was proposed as the ideal target biofluid because its composition can rapidly equilibrate with that of systemic blood, allowing the assessment of molecular concentrations that reflect full-body physiology. In the past, continuous monitoring in ISF was enabled by microneedle sensor arrays. Yet, benchmark microneedle sensors can only detect molecules that undergo redox reactions, which limits the ability to sense metabolites, biomarkers, and therapeutics that are not redox-active. To overcome this barrier, here, we expand the scope of these devices by demonstrating the first use of microneedle-supported electrochemical, aptamer-based (E-AB) sensors. This platform achieves molecular recognition based on affinity interactions, vastly expanding the scope of molecules that can be sensed. We report the fabrication of microneedle E-AB sensor arrays and a method to regenerate them for multiple uses. In addition, we demonstrate continuous molecular measurements using these sensors in flow systems in vitro using single and multiplexed microneedle array configurations. Translation of the platform to in vivo measurements is possible as we demonstrate with a first E-AB measurement in the ISF of a rodent. The encouraging results reported in this work should serve as the basis for future translation of microneedle E-AB sensor arrays to biomedical research in preclinical animal models.
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spelling pubmed-92025572023-06-02 Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring Wu, Yao Tehrani, Farshad Teymourian, Hazhir Mack, John Shaver, Alexander Reynoso, Maria Kavner, Jonathan Huang, Nickey Furmidge, Allison Duvvuri, Andrés Nie, Yuhang Laffel, Lori M. Doyle, Francis J. Patti, Mary-Elizabeth Dassau, Eyal Wang, Joseph Arroyo-Currás, Netzahualcóyotl Anal Chem [Image: see text] The ability to continuously monitor the concentration of specific molecules in the body is a long-sought goal of biomedical research. For this purpose, interstitial fluid (ISF) was proposed as the ideal target biofluid because its composition can rapidly equilibrate with that of systemic blood, allowing the assessment of molecular concentrations that reflect full-body physiology. In the past, continuous monitoring in ISF was enabled by microneedle sensor arrays. Yet, benchmark microneedle sensors can only detect molecules that undergo redox reactions, which limits the ability to sense metabolites, biomarkers, and therapeutics that are not redox-active. To overcome this barrier, here, we expand the scope of these devices by demonstrating the first use of microneedle-supported electrochemical, aptamer-based (E-AB) sensors. This platform achieves molecular recognition based on affinity interactions, vastly expanding the scope of molecules that can be sensed. We report the fabrication of microneedle E-AB sensor arrays and a method to regenerate them for multiple uses. In addition, we demonstrate continuous molecular measurements using these sensors in flow systems in vitro using single and multiplexed microneedle array configurations. Translation of the platform to in vivo measurements is possible as we demonstrate with a first E-AB measurement in the ISF of a rodent. The encouraging results reported in this work should serve as the basis for future translation of microneedle E-AB sensor arrays to biomedical research in preclinical animal models. American Chemical Society 2022-06-02 2022-06-14 /pmc/articles/PMC9202557/ /pubmed/35653647 http://dx.doi.org/10.1021/acs.analchem.2c00829 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Wu, Yao
Tehrani, Farshad
Teymourian, Hazhir
Mack, John
Shaver, Alexander
Reynoso, Maria
Kavner, Jonathan
Huang, Nickey
Furmidge, Allison
Duvvuri, Andrés
Nie, Yuhang
Laffel, Lori M.
Doyle, Francis J.
Patti, Mary-Elizabeth
Dassau, Eyal
Wang, Joseph
Arroyo-Currás, Netzahualcóyotl
Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring
title Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring
title_full Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring
title_fullStr Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring
title_full_unstemmed Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring
title_short Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring
title_sort microneedle aptamer-based sensors for continuous, real-time therapeutic drug monitoring
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202557/
https://www.ncbi.nlm.nih.gov/pubmed/35653647
http://dx.doi.org/10.1021/acs.analchem.2c00829
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