Cargando…
Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring
[Image: see text] The ability to continuously monitor the concentration of specific molecules in the body is a long-sought goal of biomedical research. For this purpose, interstitial fluid (ISF) was proposed as the ideal target biofluid because its composition can rapidly equilibrate with that of sy...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202557/ https://www.ncbi.nlm.nih.gov/pubmed/35653647 http://dx.doi.org/10.1021/acs.analchem.2c00829 |
_version_ | 1784728555044732928 |
---|---|
author | Wu, Yao Tehrani, Farshad Teymourian, Hazhir Mack, John Shaver, Alexander Reynoso, Maria Kavner, Jonathan Huang, Nickey Furmidge, Allison Duvvuri, Andrés Nie, Yuhang Laffel, Lori M. Doyle, Francis J. Patti, Mary-Elizabeth Dassau, Eyal Wang, Joseph Arroyo-Currás, Netzahualcóyotl |
author_facet | Wu, Yao Tehrani, Farshad Teymourian, Hazhir Mack, John Shaver, Alexander Reynoso, Maria Kavner, Jonathan Huang, Nickey Furmidge, Allison Duvvuri, Andrés Nie, Yuhang Laffel, Lori M. Doyle, Francis J. Patti, Mary-Elizabeth Dassau, Eyal Wang, Joseph Arroyo-Currás, Netzahualcóyotl |
author_sort | Wu, Yao |
collection | PubMed |
description | [Image: see text] The ability to continuously monitor the concentration of specific molecules in the body is a long-sought goal of biomedical research. For this purpose, interstitial fluid (ISF) was proposed as the ideal target biofluid because its composition can rapidly equilibrate with that of systemic blood, allowing the assessment of molecular concentrations that reflect full-body physiology. In the past, continuous monitoring in ISF was enabled by microneedle sensor arrays. Yet, benchmark microneedle sensors can only detect molecules that undergo redox reactions, which limits the ability to sense metabolites, biomarkers, and therapeutics that are not redox-active. To overcome this barrier, here, we expand the scope of these devices by demonstrating the first use of microneedle-supported electrochemical, aptamer-based (E-AB) sensors. This platform achieves molecular recognition based on affinity interactions, vastly expanding the scope of molecules that can be sensed. We report the fabrication of microneedle E-AB sensor arrays and a method to regenerate them for multiple uses. In addition, we demonstrate continuous molecular measurements using these sensors in flow systems in vitro using single and multiplexed microneedle array configurations. Translation of the platform to in vivo measurements is possible as we demonstrate with a first E-AB measurement in the ISF of a rodent. The encouraging results reported in this work should serve as the basis for future translation of microneedle E-AB sensor arrays to biomedical research in preclinical animal models. |
format | Online Article Text |
id | pubmed-9202557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-92025572023-06-02 Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring Wu, Yao Tehrani, Farshad Teymourian, Hazhir Mack, John Shaver, Alexander Reynoso, Maria Kavner, Jonathan Huang, Nickey Furmidge, Allison Duvvuri, Andrés Nie, Yuhang Laffel, Lori M. Doyle, Francis J. Patti, Mary-Elizabeth Dassau, Eyal Wang, Joseph Arroyo-Currás, Netzahualcóyotl Anal Chem [Image: see text] The ability to continuously monitor the concentration of specific molecules in the body is a long-sought goal of biomedical research. For this purpose, interstitial fluid (ISF) was proposed as the ideal target biofluid because its composition can rapidly equilibrate with that of systemic blood, allowing the assessment of molecular concentrations that reflect full-body physiology. In the past, continuous monitoring in ISF was enabled by microneedle sensor arrays. Yet, benchmark microneedle sensors can only detect molecules that undergo redox reactions, which limits the ability to sense metabolites, biomarkers, and therapeutics that are not redox-active. To overcome this barrier, here, we expand the scope of these devices by demonstrating the first use of microneedle-supported electrochemical, aptamer-based (E-AB) sensors. This platform achieves molecular recognition based on affinity interactions, vastly expanding the scope of molecules that can be sensed. We report the fabrication of microneedle E-AB sensor arrays and a method to regenerate them for multiple uses. In addition, we demonstrate continuous molecular measurements using these sensors in flow systems in vitro using single and multiplexed microneedle array configurations. Translation of the platform to in vivo measurements is possible as we demonstrate with a first E-AB measurement in the ISF of a rodent. The encouraging results reported in this work should serve as the basis for future translation of microneedle E-AB sensor arrays to biomedical research in preclinical animal models. American Chemical Society 2022-06-02 2022-06-14 /pmc/articles/PMC9202557/ /pubmed/35653647 http://dx.doi.org/10.1021/acs.analchem.2c00829 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Wu, Yao Tehrani, Farshad Teymourian, Hazhir Mack, John Shaver, Alexander Reynoso, Maria Kavner, Jonathan Huang, Nickey Furmidge, Allison Duvvuri, Andrés Nie, Yuhang Laffel, Lori M. Doyle, Francis J. Patti, Mary-Elizabeth Dassau, Eyal Wang, Joseph Arroyo-Currás, Netzahualcóyotl Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring |
title | Microneedle Aptamer-Based Sensors for Continuous,
Real-Time Therapeutic Drug Monitoring |
title_full | Microneedle Aptamer-Based Sensors for Continuous,
Real-Time Therapeutic Drug Monitoring |
title_fullStr | Microneedle Aptamer-Based Sensors for Continuous,
Real-Time Therapeutic Drug Monitoring |
title_full_unstemmed | Microneedle Aptamer-Based Sensors for Continuous,
Real-Time Therapeutic Drug Monitoring |
title_short | Microneedle Aptamer-Based Sensors for Continuous,
Real-Time Therapeutic Drug Monitoring |
title_sort | microneedle aptamer-based sensors for continuous,
real-time therapeutic drug monitoring |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202557/ https://www.ncbi.nlm.nih.gov/pubmed/35653647 http://dx.doi.org/10.1021/acs.analchem.2c00829 |
work_keys_str_mv | AT wuyao microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT tehranifarshad microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT teymourianhazhir microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT mackjohn microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT shaveralexander microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT reynosomaria microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT kavnerjonathan microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT huangnickey microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT furmidgeallison microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT duvvuriandres microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT nieyuhang microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT laffellorim microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT doylefrancisj microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT pattimaryelizabeth microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT dassaueyal microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT wangjoseph microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring AT arroyocurrasnetzahualcoyotl microneedleaptamerbasedsensorsforcontinuousrealtimetherapeuticdrugmonitoring |