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Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia

Despite diagnostic advances in microbiology, the etiology of neutropenic fever remains elusive in most cases. In this study, we evaluated the utility of a metagenomic shotgun sequencing based assay for detection of bacteria and viruses in blood samples of patients with febrile neutropenia. We prospe...

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Autores principales: Vijayvargiya, Prakhar, Feri, Adeline, Mairey, Mathilde, Rouillon, Cécile, Jeraldo, Patricio R., Esquer Garrigos, Zerelda, Thoendel, Matthew J., Greenwood-Quaintance, Kerryl E., Sohail, M. Rizwan, Sampathkumar, Priya, Spychalla, Megan T., Stewart, A. K., Patnaik, Mrinal M., Tande, Aaron J., Cruveiller, Stéphane, Hannet, Irene, Beurdeley, Pascale, Patel, Robin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202879/
https://www.ncbi.nlm.nih.gov/pubmed/35709201
http://dx.doi.org/10.1371/journal.pone.0269405
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author Vijayvargiya, Prakhar
Feri, Adeline
Mairey, Mathilde
Rouillon, Cécile
Jeraldo, Patricio R.
Esquer Garrigos, Zerelda
Thoendel, Matthew J.
Greenwood-Quaintance, Kerryl E.
Sohail, M. Rizwan
Sampathkumar, Priya
Spychalla, Megan T.
Stewart, A. K.
Patnaik, Mrinal M.
Tande, Aaron J.
Cruveiller, Stéphane
Hannet, Irene
Beurdeley, Pascale
Patel, Robin
author_facet Vijayvargiya, Prakhar
Feri, Adeline
Mairey, Mathilde
Rouillon, Cécile
Jeraldo, Patricio R.
Esquer Garrigos, Zerelda
Thoendel, Matthew J.
Greenwood-Quaintance, Kerryl E.
Sohail, M. Rizwan
Sampathkumar, Priya
Spychalla, Megan T.
Stewart, A. K.
Patnaik, Mrinal M.
Tande, Aaron J.
Cruveiller, Stéphane
Hannet, Irene
Beurdeley, Pascale
Patel, Robin
author_sort Vijayvargiya, Prakhar
collection PubMed
description Despite diagnostic advances in microbiology, the etiology of neutropenic fever remains elusive in most cases. In this study, we evaluated the utility of a metagenomic shotgun sequencing based assay for detection of bacteria and viruses in blood samples of patients with febrile neutropenia. We prospectively enrolled 20 acute leukemia patients and obtained blood from these patients at three time points: 1) anytime from onset of neutropenia until before development of neutropenic fever, 2) within 24 hours of onset of neutropenic fever, 3) 5–7 days after onset of neutropenic fever. Blood samples underwent sample preparation, sequencing and analysis using the iDTECT® Dx Blood v1® platform (PathoQuest, Paris, France). Clinically relevant viruses or bacteria were detected in three cases each by metagenomic shotgun sequencing and blood cultures, albeit with no concordance between the two. Further optimization of sample preparation methods and sequencing platforms is needed before widespread adoption of this technology into clinical practice.
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spelling pubmed-92028792022-06-17 Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia Vijayvargiya, Prakhar Feri, Adeline Mairey, Mathilde Rouillon, Cécile Jeraldo, Patricio R. Esquer Garrigos, Zerelda Thoendel, Matthew J. Greenwood-Quaintance, Kerryl E. Sohail, M. Rizwan Sampathkumar, Priya Spychalla, Megan T. Stewart, A. K. Patnaik, Mrinal M. Tande, Aaron J. Cruveiller, Stéphane Hannet, Irene Beurdeley, Pascale Patel, Robin PLoS One Research Article Despite diagnostic advances in microbiology, the etiology of neutropenic fever remains elusive in most cases. In this study, we evaluated the utility of a metagenomic shotgun sequencing based assay for detection of bacteria and viruses in blood samples of patients with febrile neutropenia. We prospectively enrolled 20 acute leukemia patients and obtained blood from these patients at three time points: 1) anytime from onset of neutropenia until before development of neutropenic fever, 2) within 24 hours of onset of neutropenic fever, 3) 5–7 days after onset of neutropenic fever. Blood samples underwent sample preparation, sequencing and analysis using the iDTECT® Dx Blood v1® platform (PathoQuest, Paris, France). Clinically relevant viruses or bacteria were detected in three cases each by metagenomic shotgun sequencing and blood cultures, albeit with no concordance between the two. Further optimization of sample preparation methods and sequencing platforms is needed before widespread adoption of this technology into clinical practice. Public Library of Science 2022-06-16 /pmc/articles/PMC9202879/ /pubmed/35709201 http://dx.doi.org/10.1371/journal.pone.0269405 Text en © 2022 Vijayvargiya et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Vijayvargiya, Prakhar
Feri, Adeline
Mairey, Mathilde
Rouillon, Cécile
Jeraldo, Patricio R.
Esquer Garrigos, Zerelda
Thoendel, Matthew J.
Greenwood-Quaintance, Kerryl E.
Sohail, M. Rizwan
Sampathkumar, Priya
Spychalla, Megan T.
Stewart, A. K.
Patnaik, Mrinal M.
Tande, Aaron J.
Cruveiller, Stéphane
Hannet, Irene
Beurdeley, Pascale
Patel, Robin
Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia
title Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia
title_full Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia
title_fullStr Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia
title_full_unstemmed Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia
title_short Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia
title_sort metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202879/
https://www.ncbi.nlm.nih.gov/pubmed/35709201
http://dx.doi.org/10.1371/journal.pone.0269405
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