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Optimization of a Quantitative PCR Methodology for Detection of Aspergillus spp. and Rhizopus arrhizus

INTRODUCTION: Multiplex quantitative polymerase chain reaction (qPCR) methods for the detection of Aspergillus spp. based only on SYBR Green and melting curve analysis of PCR products are difficult to develop because most targets are located within ITS regions. The aim of this study was to adapt our...

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Detalles Bibliográficos
Autores principales: Mendonça, Alexandre, Carvalho-Pereira, Joana, Franco-Duarte, Ricardo, Sampaio, Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202985/
https://www.ncbi.nlm.nih.gov/pubmed/35710958
http://dx.doi.org/10.1007/s40291-022-00595-1