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Optimization of a Quantitative PCR Methodology for Detection of Aspergillus spp. and Rhizopus arrhizus
INTRODUCTION: Multiplex quantitative polymerase chain reaction (qPCR) methods for the detection of Aspergillus spp. based only on SYBR Green and melting curve analysis of PCR products are difficult to develop because most targets are located within ITS regions. The aim of this study was to adapt our...
Autores principales: | Mendonça, Alexandre, Carvalho-Pereira, Joana, Franco-Duarte, Ricardo, Sampaio, Paula |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202985/ https://www.ncbi.nlm.nih.gov/pubmed/35710958 http://dx.doi.org/10.1007/s40291-022-00595-1 |
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