Unmixing for ultra-high-plex fluorescence imaging

Writing in Nature communications, Seo and collaborators presented PICASSO as a method to achieve 15-color imaging of spatially overlapping proteins using no reference emission spectra in a single staining and imaging round. This accessible tool has the potential to be applied to diverse applications...

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Detalles Bibliográficos
Autores principales: Enninful, Archibald, Baysoy, Alev, Fan, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Comment
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203536/
https://www.ncbi.nlm.nih.gov/pubmed/35710800
http://dx.doi.org/10.1038/s41467-022-31110-z
Descripción
Sumario:Writing in Nature communications, Seo and collaborators presented PICASSO as a method to achieve 15-color imaging of spatially overlapping proteins using no reference emission spectra in a single staining and imaging round. This accessible tool has the potential to be applied to diverse applications within the spatial biology field without neglecting accuracy.

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