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Construction of an Antibiotic-Free Vector and its Application in the Metabolic Engineering of Escherichia Coli for Polyhydroxybutyrate Production

An antibiotic- and inducer-free culture condition was proposed for polyhydroxybutyrate (PHB) production in recombinant Escherichia coli. First, antibiotic-free vectors were constructed by installing the plasmid maintenance system, alp7, hok/sok, and the hok/sok and alp7 combination into the pUC19 ve...

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Detalles Bibliográficos
Autores principales:	Liao, Ying-Cheng, Saengsawang, Boonyawee, Chen, Jun-Wei, Zhuo, Xiao-Zhen, Li, Si-Yu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204107/ https://www.ncbi.nlm.nih.gov/pubmed/35721860 http://dx.doi.org/10.3389/fbioe.2022.837944

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