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High Salt Induces a Delayed Activation of Human Neutrophils

High salt (NaCl) concentrations are found in a number of tissues under physiological and pathological conditions. Here, we analyzed the effects induced by high salt on the function of human neutrophils. The culture of neutrophils in medium supplemented with high salt (50 mM NaCl) for short periods (...

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Autores principales: Mazzitelli, Ignacio, Bleichmar, Lucía, Melucci, Claudia, Gerber, Pehuén Pereyra, Toscanini, Agustina, Cuestas, María Luján, Diaz, Fernando Erra, Geffner, Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204211/
https://www.ncbi.nlm.nih.gov/pubmed/35720394
http://dx.doi.org/10.3389/fimmu.2022.831844
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author Mazzitelli, Ignacio
Bleichmar, Lucía
Melucci, Claudia
Gerber, Pehuén Pereyra
Toscanini, Agustina
Cuestas, María Luján
Diaz, Fernando Erra
Geffner, Jorge
author_facet Mazzitelli, Ignacio
Bleichmar, Lucía
Melucci, Claudia
Gerber, Pehuén Pereyra
Toscanini, Agustina
Cuestas, María Luján
Diaz, Fernando Erra
Geffner, Jorge
author_sort Mazzitelli, Ignacio
collection PubMed
description High salt (NaCl) concentrations are found in a number of tissues under physiological and pathological conditions. Here, we analyzed the effects induced by high salt on the function of human neutrophils. The culture of neutrophils in medium supplemented with high salt (50 mM NaCl) for short periods (30-120 min) inhibited the ability of conventional agonists to induce the production of IL-8 and the activation of respiratory burst. By contrast, exposure to high salt for longer periods (6-18 h) resulted in the activation of neutrophils revealed by the production of high levels of IL-8, the activation of the respiratory burst, and a marked synergistic effect on the production of TNF-α induced by LPS. Increasing osmolarity of the culture medium by the addition of sorbitol or mannitol (100 mM) was shown to be completely unable to stimulate neutrophil responses, suggesting that high sodium but not an increased osmolarity mediates the activation on neutrophils responses. A similar biphasic effect was observed when the function of monocytes was analyzed. Short term exposure to high salt suppressed IL-8 and TNF-α production induced by LPS while culture for longer periods triggered the production of IL-8 but not TNF-α in the absence of LPS stimulation. Contradictory results have been published regarding how high salt modulates neutrophil function. Our results suggest that the modulation of neutrophil function by high salt is strongly dependent on the exposure time.
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spelling pubmed-92042112022-06-18 High Salt Induces a Delayed Activation of Human Neutrophils Mazzitelli, Ignacio Bleichmar, Lucía Melucci, Claudia Gerber, Pehuén Pereyra Toscanini, Agustina Cuestas, María Luján Diaz, Fernando Erra Geffner, Jorge Front Immunol Immunology High salt (NaCl) concentrations are found in a number of tissues under physiological and pathological conditions. Here, we analyzed the effects induced by high salt on the function of human neutrophils. The culture of neutrophils in medium supplemented with high salt (50 mM NaCl) for short periods (30-120 min) inhibited the ability of conventional agonists to induce the production of IL-8 and the activation of respiratory burst. By contrast, exposure to high salt for longer periods (6-18 h) resulted in the activation of neutrophils revealed by the production of high levels of IL-8, the activation of the respiratory burst, and a marked synergistic effect on the production of TNF-α induced by LPS. Increasing osmolarity of the culture medium by the addition of sorbitol or mannitol (100 mM) was shown to be completely unable to stimulate neutrophil responses, suggesting that high sodium but not an increased osmolarity mediates the activation on neutrophils responses. A similar biphasic effect was observed when the function of monocytes was analyzed. Short term exposure to high salt suppressed IL-8 and TNF-α production induced by LPS while culture for longer periods triggered the production of IL-8 but not TNF-α in the absence of LPS stimulation. Contradictory results have been published regarding how high salt modulates neutrophil function. Our results suggest that the modulation of neutrophil function by high salt is strongly dependent on the exposure time. Frontiers Media S.A. 2022-06-03 /pmc/articles/PMC9204211/ /pubmed/35720394 http://dx.doi.org/10.3389/fimmu.2022.831844 Text en Copyright © 2022 Mazzitelli, Bleichmar, Melucci, Gerber, Toscanini, Cuestas, Diaz and Geffner https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Mazzitelli, Ignacio
Bleichmar, Lucía
Melucci, Claudia
Gerber, Pehuén Pereyra
Toscanini, Agustina
Cuestas, María Luján
Diaz, Fernando Erra
Geffner, Jorge
High Salt Induces a Delayed Activation of Human Neutrophils
title High Salt Induces a Delayed Activation of Human Neutrophils
title_full High Salt Induces a Delayed Activation of Human Neutrophils
title_fullStr High Salt Induces a Delayed Activation of Human Neutrophils
title_full_unstemmed High Salt Induces a Delayed Activation of Human Neutrophils
title_short High Salt Induces a Delayed Activation of Human Neutrophils
title_sort high salt induces a delayed activation of human neutrophils
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204211/
https://www.ncbi.nlm.nih.gov/pubmed/35720394
http://dx.doi.org/10.3389/fimmu.2022.831844
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