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Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma

Excision repair cross-complementation group 6 like (ERCC6L) has been reported to be upregulated in a variety of malignant tumors and plays a critical oncogenic role. However, the role and molecular mechanism of ERCC6L in lung adenocarcinoma (LUAD) remain unclear, and were therefore investigated in t...

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Autores principales: Huang, Xiaoyue, Jiang, Lingyu, Lu, Sufang, Yuan, Mingqing, Lin, Hui, Li, Baijun, Wen, Zhaoke, Zhong, Yonglong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204608/
https://www.ncbi.nlm.nih.gov/pubmed/35656882
http://dx.doi.org/10.3892/or.2022.8342
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author Huang, Xiaoyue
Jiang, Lingyu
Lu, Sufang
Yuan, Mingqing
Lin, Hui
Li, Baijun
Wen, Zhaoke
Zhong, Yonglong
author_facet Huang, Xiaoyue
Jiang, Lingyu
Lu, Sufang
Yuan, Mingqing
Lin, Hui
Li, Baijun
Wen, Zhaoke
Zhong, Yonglong
author_sort Huang, Xiaoyue
collection PubMed
description Excision repair cross-complementation group 6 like (ERCC6L) has been reported to be upregulated in a variety of malignant tumors and plays a critical oncogenic role. However, the role and molecular mechanism of ERCC6L in lung adenocarcinoma (LUAD) remain unclear, and were therefore investigated in the present study. Clinical data of patients with LUAD were obtained and bioinformatics analysis was performed to investigate the expression characteristics, prognostic value, and biological function of ERCC6L. In addition, cell function experiments were performed to detect the effect of ERCC6L silencing on the biological behavior of LUAD cells. The results revealed that ERCC6L expression was significantly higher in LUAD tissues vs. normal lung tissues and closely associated with nodal invasion, advanced clinical stage and survival in LUAD. Overexpression of ERCC6L was an independent prognostic biomarker of overall survival, progression-free interval, and disease-specific survival in patients with LUAD. DNA amplification and low methylation levels of ERCC6L suggested regulation at both the genetic and epigenetic levels. The most significant positive genes co-expressed with ERCC6L were mainly enriched in the cell cycle signaling pathway. The major functions of ERCC6L in LUAD cells were positively correlated with the cell cycle, DNA damage, DNA repair, proliferation, invasion and epithelial-mesenchymal transition (EMT). Knockdown of ERCC6L inhibited the proliferative, migratory and invasive abilities of A549 and PC9 cells. It also promoted cell apoptosis, and led to cell cycle arrest in the S phase. ERCC6L may regulate the EMT process through the Wnt/β-catenin and Wnt/Notch 3 signaling pathways, thus regulating the tumorigenesis and progression of LUAD. The overexpression of ERCC6L may be a biological indicator for the diagnosis and prognosis of LUAD. ERCC6L may be a novel molecular target for the treatment of lung cancer.
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spelling pubmed-92046082022-06-22 Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma Huang, Xiaoyue Jiang, Lingyu Lu, Sufang Yuan, Mingqing Lin, Hui Li, Baijun Wen, Zhaoke Zhong, Yonglong Oncol Rep Articles Excision repair cross-complementation group 6 like (ERCC6L) has been reported to be upregulated in a variety of malignant tumors and plays a critical oncogenic role. However, the role and molecular mechanism of ERCC6L in lung adenocarcinoma (LUAD) remain unclear, and were therefore investigated in the present study. Clinical data of patients with LUAD were obtained and bioinformatics analysis was performed to investigate the expression characteristics, prognostic value, and biological function of ERCC6L. In addition, cell function experiments were performed to detect the effect of ERCC6L silencing on the biological behavior of LUAD cells. The results revealed that ERCC6L expression was significantly higher in LUAD tissues vs. normal lung tissues and closely associated with nodal invasion, advanced clinical stage and survival in LUAD. Overexpression of ERCC6L was an independent prognostic biomarker of overall survival, progression-free interval, and disease-specific survival in patients with LUAD. DNA amplification and low methylation levels of ERCC6L suggested regulation at both the genetic and epigenetic levels. The most significant positive genes co-expressed with ERCC6L were mainly enriched in the cell cycle signaling pathway. The major functions of ERCC6L in LUAD cells were positively correlated with the cell cycle, DNA damage, DNA repair, proliferation, invasion and epithelial-mesenchymal transition (EMT). Knockdown of ERCC6L inhibited the proliferative, migratory and invasive abilities of A549 and PC9 cells. It also promoted cell apoptosis, and led to cell cycle arrest in the S phase. ERCC6L may regulate the EMT process through the Wnt/β-catenin and Wnt/Notch 3 signaling pathways, thus regulating the tumorigenesis and progression of LUAD. The overexpression of ERCC6L may be a biological indicator for the diagnosis and prognosis of LUAD. ERCC6L may be a novel molecular target for the treatment of lung cancer. D.A. Spandidos 2022-06-02 /pmc/articles/PMC9204608/ /pubmed/35656882 http://dx.doi.org/10.3892/or.2022.8342 Text en Copyright: © Huang et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Huang, Xiaoyue
Jiang, Lingyu
Lu, Sufang
Yuan, Mingqing
Lin, Hui
Li, Baijun
Wen, Zhaoke
Zhong, Yonglong
Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma
title Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma
title_full Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma
title_fullStr Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma
title_full_unstemmed Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma
title_short Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma
title_sort overexpression of ercc6l correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204608/
https://www.ncbi.nlm.nih.gov/pubmed/35656882
http://dx.doi.org/10.3892/or.2022.8342
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