A combined approach to evaluate total phosphorus/inorganic phosphate levels in plants

Inorganic phosphate (Pi) and phosphorus (P) homeostasis are essential for plant growth and yield, and reliable detection of dynamic Pi/P in different tissues is important for studying their biological functions. Here, we report a combined protocol for rapid determination of Pi/P levels. We first per...

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Detalles Bibliográficos
Autores principales: Ma, Bin, Liu, Yu, Li, Xiaoyuan, Fang, Zijun, Zhang, Lin, He, Zuhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204740/
https://www.ncbi.nlm.nih.gov/pubmed/35719721
http://dx.doi.org/10.1016/j.xpro.2022.101456
Descripción
Sumario:Inorganic phosphate (Pi) and phosphorus (P) homeostasis are essential for plant growth and yield, and reliable detection of dynamic Pi/P in different tissues is important for studying their biological functions. Here, we report a combined protocol for rapid determination of Pi/P levels. We first perform (31)P NMR assay to reveal the intracellular Pi distribution and then dissect the level of Pi/P by the chromogenic reaction and ICP-MS analysis. Finally, we take μXRF element fluorescence assay to achieve the visual P distribution. For complete details on the use and execution of this protocol, please refer to Ma et al. (2021).

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