A combined approach to evaluate total phosphorus/inorganic phosphate levels in plants

Inorganic phosphate (Pi) and phosphorus (P) homeostasis are essential for plant growth and yield, and reliable detection of dynamic Pi/P in different tissues is important for studying their biological functions. Here, we report a combined protocol for rapid determination of Pi/P levels. We first per...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Bin, Liu, Yu, Li, Xiaoyuan, Fang, Zijun, Zhang, Lin, He, Zuhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204740/
https://www.ncbi.nlm.nih.gov/pubmed/35719721
http://dx.doi.org/10.1016/j.xpro.2022.101456
_version_ 1784728991063605248
author Ma, Bin
Liu, Yu
Li, Xiaoyuan
Fang, Zijun
Zhang, Lin
He, Zuhua
author_facet Ma, Bin
Liu, Yu
Li, Xiaoyuan
Fang, Zijun
Zhang, Lin
He, Zuhua
author_sort Ma, Bin
collection PubMed
description Inorganic phosphate (Pi) and phosphorus (P) homeostasis are essential for plant growth and yield, and reliable detection of dynamic Pi/P in different tissues is important for studying their biological functions. Here, we report a combined protocol for rapid determination of Pi/P levels. We first perform (31)P NMR assay to reveal the intracellular Pi distribution and then dissect the level of Pi/P by the chromogenic reaction and ICP-MS analysis. Finally, we take μXRF element fluorescence assay to achieve the visual P distribution. For complete details on the use and execution of this protocol, please refer to Ma et al. (2021).
format Online
Article
Text
id pubmed-9204740
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-92047402022-06-18 A combined approach to evaluate total phosphorus/inorganic phosphate levels in plants Ma, Bin Liu, Yu Li, Xiaoyuan Fang, Zijun Zhang, Lin He, Zuhua STAR Protoc Protocol Inorganic phosphate (Pi) and phosphorus (P) homeostasis are essential for plant growth and yield, and reliable detection of dynamic Pi/P in different tissues is important for studying their biological functions. Here, we report a combined protocol for rapid determination of Pi/P levels. We first perform (31)P NMR assay to reveal the intracellular Pi distribution and then dissect the level of Pi/P by the chromogenic reaction and ICP-MS analysis. Finally, we take μXRF element fluorescence assay to achieve the visual P distribution. For complete details on the use and execution of this protocol, please refer to Ma et al. (2021). Elsevier 2022-06-14 /pmc/articles/PMC9204740/ /pubmed/35719721 http://dx.doi.org/10.1016/j.xpro.2022.101456 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ma, Bin
Liu, Yu
Li, Xiaoyuan
Fang, Zijun
Zhang, Lin
He, Zuhua
A combined approach to evaluate total phosphorus/inorganic phosphate levels in plants
title A combined approach to evaluate total phosphorus/inorganic phosphate levels in plants
title_full A combined approach to evaluate total phosphorus/inorganic phosphate levels in plants
title_fullStr A combined approach to evaluate total phosphorus/inorganic phosphate levels in plants
title_full_unstemmed A combined approach to evaluate total phosphorus/inorganic phosphate levels in plants
title_short A combined approach to evaluate total phosphorus/inorganic phosphate levels in plants
title_sort combined approach to evaluate total phosphorus/inorganic phosphate levels in plants
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204740/
https://www.ncbi.nlm.nih.gov/pubmed/35719721
http://dx.doi.org/10.1016/j.xpro.2022.101456
work_keys_str_mv AT mabin acombinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants
AT liuyu acombinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants
AT lixiaoyuan acombinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants
AT fangzijun acombinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants
AT zhanglin acombinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants
AT hezuhua acombinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants
AT mabin combinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants
AT liuyu combinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants
AT lixiaoyuan combinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants
AT fangzijun combinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants
AT zhanglin combinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants
AT hezuhua combinedapproachtoevaluatetotalphosphorusinorganicphosphatelevelsinplants

Ejemplares similares