CRISPR/Cas9-mediated tissue-specific knockout and cDNA rescue using sgRNAs that target exon-intron junctions in Drosophila melanogaster

In this protocol, we take CRISPR/Cas9 and Gal4/UAS approaches to achieve tissue-specific knockout in parallel with rescue of the knockout by cDNA expression in Drosophila. We demonstrate that guide RNAs targeting the exon-intron junction of target genes cleave the genomic locus of the genes, but not...

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Detalles Bibliográficos
Autores principales: Chilian, Madison, Vargas Parra, Karen, Sandoval, Abigail, Ramirez, Juan, Yoon, Wan Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204798/
https://www.ncbi.nlm.nih.gov/pubmed/35719725
http://dx.doi.org/10.1016/j.xpro.2022.101465

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