Cargando…

Inducing Tolerance to Abiotic Stress in Hordeum vulgare L. by Halotolerant Endophytic Fungi Associated With Salt Lake Plants

A characteristic trait of plants living in harsh environments is their association with fungal endophytes, which enable them to survive under extreme stress. Abiotic stress resistance in agro-ecosystems, particularly in arid and semi-arid regions, can be increased by inoculating these fungal endophy...

Descripción completa

Detalles Bibliográficos
Autores principales: Hosseyni Moghaddam, Mahdieh S., Safaie, Naser, Rahimlou, Saleh, Hagh-Doust, Niloufar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205400/
https://www.ncbi.nlm.nih.gov/pubmed/35722330
http://dx.doi.org/10.3389/fmicb.2022.906365
_version_ 1784729121732952064
author Hosseyni Moghaddam, Mahdieh S.
Safaie, Naser
Rahimlou, Saleh
Hagh-Doust, Niloufar
author_facet Hosseyni Moghaddam, Mahdieh S.
Safaie, Naser
Rahimlou, Saleh
Hagh-Doust, Niloufar
author_sort Hosseyni Moghaddam, Mahdieh S.
collection PubMed
description A characteristic trait of plants living in harsh environments is their association with fungal endophytes, which enable them to survive under extreme stress. Abiotic stress resistance in agro-ecosystems, particularly in arid and semi-arid regions, can be increased by inoculating these fungal endophytes on plants other than their original hosts. The present study is therefore focused on the possible role of three halotolerant endophytic fungi, i.e., Periconia macrospinosa, Neocamarosporium goegapense, and N. chichastianum, isolated from roots of salt lake plants growing in the central desert of Iran, in alleviating the adverse effects of salinity and drought stresses on barley under greenhouse conditions. To perform this experiment, a randomized block design was applied with three factors: fungi (four levels including three halotolerant endophytic species and control), salinity (three levels including 8, 12, and 16 dS/m), and drought (four levels including 100, 80, 60, 40 percent field capacity). All plants were measured for growth characteristics, chlorophyll concentration, proline content, and antioxidant enzyme activities. A three-way analysis of variance indicated that all three fungal endophytes, to varying extents, induced the barley plants’ resistance to salinity and drought, and their combined effects. Additionally, we found that fungal endophytes were more effective when the barley plants were subjected to higher levels of salinity and drought. Under the stress of salinity and drought, a strong relationship between inoculation of fungal endophytes and enhancement of biomass, shoot length, chlorophyll concentration, proline content, and activity of catalase, peroxidase, and superoxide dismutase was indicated. We discussed that increased root growth, proline content, and antioxidant enzyme activity are the main physiological and biochemical mechanisms causing stress resistance in barley plants inoculated with endophytes. Our research findings illustrate that fungal endophytes have a substantial potential for increasing abiotic stress tolerance in barley plants, which can be applied in agricultural ecosystems.
format Online
Article
Text
id pubmed-9205400
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92054002022-06-18 Inducing Tolerance to Abiotic Stress in Hordeum vulgare L. by Halotolerant Endophytic Fungi Associated With Salt Lake Plants Hosseyni Moghaddam, Mahdieh S. Safaie, Naser Rahimlou, Saleh Hagh-Doust, Niloufar Front Microbiol Microbiology A characteristic trait of plants living in harsh environments is their association with fungal endophytes, which enable them to survive under extreme stress. Abiotic stress resistance in agro-ecosystems, particularly in arid and semi-arid regions, can be increased by inoculating these fungal endophytes on plants other than their original hosts. The present study is therefore focused on the possible role of three halotolerant endophytic fungi, i.e., Periconia macrospinosa, Neocamarosporium goegapense, and N. chichastianum, isolated from roots of salt lake plants growing in the central desert of Iran, in alleviating the adverse effects of salinity and drought stresses on barley under greenhouse conditions. To perform this experiment, a randomized block design was applied with three factors: fungi (four levels including three halotolerant endophytic species and control), salinity (three levels including 8, 12, and 16 dS/m), and drought (four levels including 100, 80, 60, 40 percent field capacity). All plants were measured for growth characteristics, chlorophyll concentration, proline content, and antioxidant enzyme activities. A three-way analysis of variance indicated that all three fungal endophytes, to varying extents, induced the barley plants’ resistance to salinity and drought, and their combined effects. Additionally, we found that fungal endophytes were more effective when the barley plants were subjected to higher levels of salinity and drought. Under the stress of salinity and drought, a strong relationship between inoculation of fungal endophytes and enhancement of biomass, shoot length, chlorophyll concentration, proline content, and activity of catalase, peroxidase, and superoxide dismutase was indicated. We discussed that increased root growth, proline content, and antioxidant enzyme activity are the main physiological and biochemical mechanisms causing stress resistance in barley plants inoculated with endophytes. Our research findings illustrate that fungal endophytes have a substantial potential for increasing abiotic stress tolerance in barley plants, which can be applied in agricultural ecosystems. Frontiers Media S.A. 2022-05-20 /pmc/articles/PMC9205400/ /pubmed/35722330 http://dx.doi.org/10.3389/fmicb.2022.906365 Text en Copyright © 2022 Hosseyni Moghaddam, Safaie, Rahimlou and Hagh-Doust. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hosseyni Moghaddam, Mahdieh S.
Safaie, Naser
Rahimlou, Saleh
Hagh-Doust, Niloufar
Inducing Tolerance to Abiotic Stress in Hordeum vulgare L. by Halotolerant Endophytic Fungi Associated With Salt Lake Plants
title Inducing Tolerance to Abiotic Stress in Hordeum vulgare L. by Halotolerant Endophytic Fungi Associated With Salt Lake Plants
title_full Inducing Tolerance to Abiotic Stress in Hordeum vulgare L. by Halotolerant Endophytic Fungi Associated With Salt Lake Plants
title_fullStr Inducing Tolerance to Abiotic Stress in Hordeum vulgare L. by Halotolerant Endophytic Fungi Associated With Salt Lake Plants
title_full_unstemmed Inducing Tolerance to Abiotic Stress in Hordeum vulgare L. by Halotolerant Endophytic Fungi Associated With Salt Lake Plants
title_short Inducing Tolerance to Abiotic Stress in Hordeum vulgare L. by Halotolerant Endophytic Fungi Associated With Salt Lake Plants
title_sort inducing tolerance to abiotic stress in hordeum vulgare l. by halotolerant endophytic fungi associated with salt lake plants
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205400/
https://www.ncbi.nlm.nih.gov/pubmed/35722330
http://dx.doi.org/10.3389/fmicb.2022.906365
work_keys_str_mv AT hosseynimoghaddammahdiehs inducingtolerancetoabioticstressinhordeumvulgarelbyhalotolerantendophyticfungiassociatedwithsaltlakeplants
AT safaienaser inducingtolerancetoabioticstressinhordeumvulgarelbyhalotolerantendophyticfungiassociatedwithsaltlakeplants
AT rahimlousaleh inducingtolerancetoabioticstressinhordeumvulgarelbyhalotolerantendophyticfungiassociatedwithsaltlakeplants
AT haghdoustniloufar inducingtolerancetoabioticstressinhordeumvulgarelbyhalotolerantendophyticfungiassociatedwithsaltlakeplants