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Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay
We developed two quantitative PCR-based assays to detect SARS-CoV-2-specific T cell immunity: qTACT and dqTACT. The assays quantify CXCL10 mRNA, after incubation of whole blood with viral peptides, as a proxy of an antigen-specific T cell response, and will allow population-level monitoring of cellu...
| Formato: | Online Artículo Texto |
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| Lenguaje: | English |
| Publicado: |
Nature Publishing Group US
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205411/ https://www.ncbi.nlm.nih.gov/pubmed/35715589 http://dx.doi.org/10.1038/s41587-022-01358-3 |
| _version_ | 1784729124903845888 |
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| collection | PubMed |
| description | We developed two quantitative PCR-based assays to detect SARS-CoV-2-specific T cell immunity: qTACT and dqTACT. The assays quantify CXCL10 mRNA, after incubation of whole blood with viral peptides, as a proxy of an antigen-specific T cell response, and will allow population-level monitoring of cellular immunity to SARS-CoV-2. |
| format | Online Article Text |
| id | pubmed-9205411 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Nature Publishing Group US |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-92054112022-06-21 Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay Nat Biotechnol Research Briefing We developed two quantitative PCR-based assays to detect SARS-CoV-2-specific T cell immunity: qTACT and dqTACT. The assays quantify CXCL10 mRNA, after incubation of whole blood with viral peptides, as a proxy of an antigen-specific T cell response, and will allow population-level monitoring of cellular immunity to SARS-CoV-2. Nature Publishing Group US 2022-06-17 2022 /pmc/articles/PMC9205411/ /pubmed/35715589 http://dx.doi.org/10.1038/s41587-022-01358-3 Text en © Springer Nature America, Inc. 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
| spellingShingle | Research Briefing Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay |
| title | Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay |
| title_full | Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay |
| title_fullStr | Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay |
| title_full_unstemmed | Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay |
| title_short | Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay |
| title_sort | measuring sars-cov-2 t cell immunity with a scalable qpcr-based assay |
| topic | Research Briefing |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205411/ https://www.ncbi.nlm.nih.gov/pubmed/35715589 http://dx.doi.org/10.1038/s41587-022-01358-3 |