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Novel Fusarium wilt resistance genes uncovered in natural and cultivated strawberry populations are found on three non-homoeologous chromosomes

KEY MESSAGE: Several Fusarium wilt resistance genes were discovered, genetically and physically mapped, and rapidly deployed via marker-assisted selection to develop cultivars resistant to Fusarium oxysporum f. sp. fragariae, a devastating soil-borne pathogen of strawberry. ABSTRACT: Fusarium wilt,...

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Autores principales: Pincot, Dominique D. A., Feldmann, Mitchell J., Hardigan, Michael A., Vachev, Mishi V., Henry, Peter M., Gordon, Thomas R., Bjornson, Marta, Rodriguez, Alan, Cobo, Nicolas, Famula, Randi A., Cole, Glenn S., Coaker, Gitta L., Knapp, Steven J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205853/
https://www.ncbi.nlm.nih.gov/pubmed/35583656
http://dx.doi.org/10.1007/s00122-022-04102-2
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author Pincot, Dominique D. A.
Feldmann, Mitchell J.
Hardigan, Michael A.
Vachev, Mishi V.
Henry, Peter M.
Gordon, Thomas R.
Bjornson, Marta
Rodriguez, Alan
Cobo, Nicolas
Famula, Randi A.
Cole, Glenn S.
Coaker, Gitta L.
Knapp, Steven J.
author_facet Pincot, Dominique D. A.
Feldmann, Mitchell J.
Hardigan, Michael A.
Vachev, Mishi V.
Henry, Peter M.
Gordon, Thomas R.
Bjornson, Marta
Rodriguez, Alan
Cobo, Nicolas
Famula, Randi A.
Cole, Glenn S.
Coaker, Gitta L.
Knapp, Steven J.
author_sort Pincot, Dominique D. A.
collection PubMed
description KEY MESSAGE: Several Fusarium wilt resistance genes were discovered, genetically and physically mapped, and rapidly deployed via marker-assisted selection to develop cultivars resistant to Fusarium oxysporum f. sp. fragariae, a devastating soil-borne pathogen of strawberry. ABSTRACT: Fusarium wilt, a soilborne disease caused by Fusarium oxysporum f. sp. fragariae, poses a significant threat to strawberry (Fragaria [Formula: see text] ananassa) production in many parts of the world. This pathogen causes wilting, collapse, and death in susceptible genotypes. We previously identified a dominant gene (FW1) on chromosome 2B that confers resistance to race 1 of the pathogen, and hypothesized that gene-for-gene resistance to Fusarium wilt was widespread in strawberry. To explore this, a genetically diverse collection of heirloom and modern cultivars and octoploid ecotypes were screened for resistance to Fusarium wilt races 1 and 2. Here, we show that resistance to both races is widespread in natural and domesticated populations and that resistance to race 1 is conferred by partially to completely dominant alleles among loci (FW1, FW2, FW3, FW4, and FW5) found on three non-homoeologous chromosomes (1A, 2B, and 6B). The underlying genes have not yet been cloned and functionally characterized; however, plausible candidates were identified that encode pattern recognition receptors or other proteins known to confer gene-for-gene resistance in plants. High-throughput genotyping assays for SNPs in linkage disequilibrium with FW1-FW5 were developed to facilitate marker-assisted selection and accelerate the development of race 1 resistant cultivars. This study laid the foundation for identifying the genes encoded by FW1-FW5, in addition to exploring the genetics of resistance to race 2 and other races of the pathogen, as a precaution to averting a Fusarium wilt pandemic.
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spelling pubmed-92058532022-06-19 Novel Fusarium wilt resistance genes uncovered in natural and cultivated strawberry populations are found on three non-homoeologous chromosomes Pincot, Dominique D. A. Feldmann, Mitchell J. Hardigan, Michael A. Vachev, Mishi V. Henry, Peter M. Gordon, Thomas R. Bjornson, Marta Rodriguez, Alan Cobo, Nicolas Famula, Randi A. Cole, Glenn S. Coaker, Gitta L. Knapp, Steven J. Theor Appl Genet Original Article KEY MESSAGE: Several Fusarium wilt resistance genes were discovered, genetically and physically mapped, and rapidly deployed via marker-assisted selection to develop cultivars resistant to Fusarium oxysporum f. sp. fragariae, a devastating soil-borne pathogen of strawberry. ABSTRACT: Fusarium wilt, a soilborne disease caused by Fusarium oxysporum f. sp. fragariae, poses a significant threat to strawberry (Fragaria [Formula: see text] ananassa) production in many parts of the world. This pathogen causes wilting, collapse, and death in susceptible genotypes. We previously identified a dominant gene (FW1) on chromosome 2B that confers resistance to race 1 of the pathogen, and hypothesized that gene-for-gene resistance to Fusarium wilt was widespread in strawberry. To explore this, a genetically diverse collection of heirloom and modern cultivars and octoploid ecotypes were screened for resistance to Fusarium wilt races 1 and 2. Here, we show that resistance to both races is widespread in natural and domesticated populations and that resistance to race 1 is conferred by partially to completely dominant alleles among loci (FW1, FW2, FW3, FW4, and FW5) found on three non-homoeologous chromosomes (1A, 2B, and 6B). The underlying genes have not yet been cloned and functionally characterized; however, plausible candidates were identified that encode pattern recognition receptors or other proteins known to confer gene-for-gene resistance in plants. High-throughput genotyping assays for SNPs in linkage disequilibrium with FW1-FW5 were developed to facilitate marker-assisted selection and accelerate the development of race 1 resistant cultivars. This study laid the foundation for identifying the genes encoded by FW1-FW5, in addition to exploring the genetics of resistance to race 2 and other races of the pathogen, as a precaution to averting a Fusarium wilt pandemic. Springer Berlin Heidelberg 2022-05-18 2022 /pmc/articles/PMC9205853/ /pubmed/35583656 http://dx.doi.org/10.1007/s00122-022-04102-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Pincot, Dominique D. A.
Feldmann, Mitchell J.
Hardigan, Michael A.
Vachev, Mishi V.
Henry, Peter M.
Gordon, Thomas R.
Bjornson, Marta
Rodriguez, Alan
Cobo, Nicolas
Famula, Randi A.
Cole, Glenn S.
Coaker, Gitta L.
Knapp, Steven J.
Novel Fusarium wilt resistance genes uncovered in natural and cultivated strawberry populations are found on three non-homoeologous chromosomes
title Novel Fusarium wilt resistance genes uncovered in natural and cultivated strawberry populations are found on three non-homoeologous chromosomes
title_full Novel Fusarium wilt resistance genes uncovered in natural and cultivated strawberry populations are found on three non-homoeologous chromosomes
title_fullStr Novel Fusarium wilt resistance genes uncovered in natural and cultivated strawberry populations are found on three non-homoeologous chromosomes
title_full_unstemmed Novel Fusarium wilt resistance genes uncovered in natural and cultivated strawberry populations are found on three non-homoeologous chromosomes
title_short Novel Fusarium wilt resistance genes uncovered in natural and cultivated strawberry populations are found on three non-homoeologous chromosomes
title_sort novel fusarium wilt resistance genes uncovered in natural and cultivated strawberry populations are found on three non-homoeologous chromosomes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205853/
https://www.ncbi.nlm.nih.gov/pubmed/35583656
http://dx.doi.org/10.1007/s00122-022-04102-2
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