Strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration

Repairing of large bone injuries is an important problem in bone regeneration field. Thus, developing new therapeutic approaches such as tissue engineering using 3D scaffolds is necessary. Incorporation of some bioactive materials and trace elements can improve scaffold properties. We made chitosan/...

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Autores principales: Manoochehri, Hamed, Ghorbani, Masoud, Moosazadeh Moghaddam, Mehrdad, Nourani, Mohammad Reza, Makvandi, Pooyan, Sharifi, Esmaeel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205926/
https://www.ncbi.nlm.nih.gov/pubmed/35715472
http://dx.doi.org/10.1038/s41598-022-14329-0
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author Manoochehri, Hamed
Ghorbani, Masoud
Moosazadeh Moghaddam, Mehrdad
Nourani, Mohammad Reza
Makvandi, Pooyan
Sharifi, Esmaeel
author_facet Manoochehri, Hamed
Ghorbani, Masoud
Moosazadeh Moghaddam, Mehrdad
Nourani, Mohammad Reza
Makvandi, Pooyan
Sharifi, Esmaeel
author_sort Manoochehri, Hamed
collection PubMed
description Repairing of large bone injuries is an important problem in bone regeneration field. Thus, developing new therapeutic approaches such as tissue engineering using 3D scaffolds is necessary. Incorporation of some bioactive materials and trace elements can improve scaffold properties. We made chitosan/alginate/strontium-doped bioglass composite scaffolds with optimized properties for bone tissue engineering. Bioglass (BG) and Sr-doped bioglasses (Sr-BG) were synthesized using Sol–Gel method. Alginate-Chitosan (Alg/Cs) scaffold and scaffolds containing different ratio (10%, 20% and 30%) of BG (Alg/Cs/BG10, 20, 30) or Sr-BG (Alg/Cs/Sr-BG10, 20, 30) were fabricated using freeze drying method. Characterization of bioglasses/scaffolds was done using zeta sizer, FTIR, XRD, (FE) SEM and EDS. Also, mechanical strength, antibacterial effect degradation and swelling profile of scaffolds were evaluated. Bone differentiation efficiency and viability of MSCs on scaffolds were determined by Alizarin Red, ALP and MTT methods. Cell toxicity and antibacterial effect of bioglasses were determined using MTT, MIC and MBC methods. Incorporation of BG into Alg/Cs scaffolds amplified biomineralization and mechanical properties along with improved swelling ratio, degradation profile and cell differentiation. Mechanical strength and cell differentiation efficiency of Alg/Cs/BG20 scaffold was considerably higher than scaffolds with lower or higher BG concentrations. Alg/Cs/Sr-BG scaffolds had higher mechanical stability and more differentiation efficiency in comparison with Alg/Cs and Alg/Cs/BG scaffolds. Also, Mechanical strength and cell differentiation efficiency of Alg/Cs/Sr-BG20 scaffold was considerably higher than scaffolds with various Sr-BG concentrations. Biomineralization of Alg/Cs/BG scaffolds slightly was higher than Alg/Cs/Sr-BG scaffolds. Overall, we concluded that Alg/Cs/Sr-BG20 scaffolds are more suitable for repairing bone major injuries.
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spelling pubmed-92059262022-06-19 Strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration Manoochehri, Hamed Ghorbani, Masoud Moosazadeh Moghaddam, Mehrdad Nourani, Mohammad Reza Makvandi, Pooyan Sharifi, Esmaeel Sci Rep Article Repairing of large bone injuries is an important problem in bone regeneration field. Thus, developing new therapeutic approaches such as tissue engineering using 3D scaffolds is necessary. Incorporation of some bioactive materials and trace elements can improve scaffold properties. We made chitosan/alginate/strontium-doped bioglass composite scaffolds with optimized properties for bone tissue engineering. Bioglass (BG) and Sr-doped bioglasses (Sr-BG) were synthesized using Sol–Gel method. Alginate-Chitosan (Alg/Cs) scaffold and scaffolds containing different ratio (10%, 20% and 30%) of BG (Alg/Cs/BG10, 20, 30) or Sr-BG (Alg/Cs/Sr-BG10, 20, 30) were fabricated using freeze drying method. Characterization of bioglasses/scaffolds was done using zeta sizer, FTIR, XRD, (FE) SEM and EDS. Also, mechanical strength, antibacterial effect degradation and swelling profile of scaffolds were evaluated. Bone differentiation efficiency and viability of MSCs on scaffolds were determined by Alizarin Red, ALP and MTT methods. Cell toxicity and antibacterial effect of bioglasses were determined using MTT, MIC and MBC methods. Incorporation of BG into Alg/Cs scaffolds amplified biomineralization and mechanical properties along with improved swelling ratio, degradation profile and cell differentiation. Mechanical strength and cell differentiation efficiency of Alg/Cs/BG20 scaffold was considerably higher than scaffolds with lower or higher BG concentrations. Alg/Cs/Sr-BG scaffolds had higher mechanical stability and more differentiation efficiency in comparison with Alg/Cs and Alg/Cs/BG scaffolds. Also, Mechanical strength and cell differentiation efficiency of Alg/Cs/Sr-BG20 scaffold was considerably higher than scaffolds with various Sr-BG concentrations. Biomineralization of Alg/Cs/BG scaffolds slightly was higher than Alg/Cs/Sr-BG scaffolds. Overall, we concluded that Alg/Cs/Sr-BG20 scaffolds are more suitable for repairing bone major injuries. Nature Publishing Group UK 2022-06-17 /pmc/articles/PMC9205926/ /pubmed/35715472 http://dx.doi.org/10.1038/s41598-022-14329-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Manoochehri, Hamed
Ghorbani, Masoud
Moosazadeh Moghaddam, Mehrdad
Nourani, Mohammad Reza
Makvandi, Pooyan
Sharifi, Esmaeel
Strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration
title Strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration
title_full Strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration
title_fullStr Strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration
title_full_unstemmed Strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration
title_short Strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration
title_sort strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205926/
https://www.ncbi.nlm.nih.gov/pubmed/35715472
http://dx.doi.org/10.1038/s41598-022-14329-0
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