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CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing

In recent years, the number of tools and techniques that enable genetic material to be added, removed or altered at specific locations in the genome has increased significantly. The objective is to know the structure of genomes, the function of genes and improve gene therapy. In this work we intend...

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Autores principales: Ferreira, Patrick, Choupina, Altino Branco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206401/
https://www.ncbi.nlm.nih.gov/pubmed/35716290
http://dx.doi.org/10.1007/s11033-022-07442-w
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author Ferreira, Patrick
Choupina, Altino Branco
author_facet Ferreira, Patrick
Choupina, Altino Branco
author_sort Ferreira, Patrick
collection PubMed
description In recent years, the number of tools and techniques that enable genetic material to be added, removed or altered at specific locations in the genome has increased significantly. The objective is to know the structure of genomes, the function of genes and improve gene therapy. In this work we intend to explain the functioning of the CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9) and the advantages that this technique may have compared to previously developed techniques, such as RNA interference (RNAi), Zinc Finger Nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) in gene and genome editing. We will start with the story of the discovery, then its biological function in the adaptive immune system of bacteria against bacteriophage attack, and ending with a description of the mechanism of action and its use in gene editing. We will also discuss other Cas enzymes with great potential for use in genome editing as an alternative to Cas9. CRISPR/Cas9 is a simple, inexpensive, and effective technique for gene editing with multiple applications from the development of functional genomics and epigenetics. This technique will, in the near future, have great applications in the development of cell models for use in medical and pharmaceutical processes, in targeted therapy, and improvement of agricultural and environmental species.
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spelling pubmed-92064012022-06-21 CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing Ferreira, Patrick Choupina, Altino Branco Mol Biol Rep Original Article In recent years, the number of tools and techniques that enable genetic material to be added, removed or altered at specific locations in the genome has increased significantly. The objective is to know the structure of genomes, the function of genes and improve gene therapy. In this work we intend to explain the functioning of the CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9) and the advantages that this technique may have compared to previously developed techniques, such as RNA interference (RNAi), Zinc Finger Nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) in gene and genome editing. We will start with the story of the discovery, then its biological function in the adaptive immune system of bacteria against bacteriophage attack, and ending with a description of the mechanism of action and its use in gene editing. We will also discuss other Cas enzymes with great potential for use in genome editing as an alternative to Cas9. CRISPR/Cas9 is a simple, inexpensive, and effective technique for gene editing with multiple applications from the development of functional genomics and epigenetics. This technique will, in the near future, have great applications in the development of cell models for use in medical and pharmaceutical processes, in targeted therapy, and improvement of agricultural and environmental species. Springer Netherlands 2022-06-18 2022 /pmc/articles/PMC9206401/ /pubmed/35716290 http://dx.doi.org/10.1007/s11033-022-07442-w Text en © The Author(s), under exclusive licence to Springer Nature B.V. 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Ferreira, Patrick
Choupina, Altino Branco
CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing
title CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing
title_full CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing
title_fullStr CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing
title_full_unstemmed CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing
title_short CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing
title_sort crispr/cas9 a simple, inexpensive and effective technique for gene editing
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206401/
https://www.ncbi.nlm.nih.gov/pubmed/35716290
http://dx.doi.org/10.1007/s11033-022-07442-w
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