Effect of CDCA5 on Proliferation and Metastasis of Triple Negative Breast Cancer Cells under shRNA Interference Technology

OBJECTIVE: It was to explore the effect of cell division cycle associated 5 (CDCA5) under shRNA interference on proliferation and metastasis of triple negative breast cancer (TNBC) cells. METHODS: MDA-ME-231 and BT549 cells were selected as the research objects. According to the different interference methods and CDCA5 interference sequences, they were divided into the interference group 1(MDA-ME-231), the interference group 2(MDA-ME-231), the interference group 1(BT549), the interference group 2(BT549) (using shRNA technology), the control group (MDA-ME-231), and the control group (BT549) (breast cancer cells under normal culture conditions). MCF10A cells were routinely cultured as the negative control group to analyze the effect of CDCA5 expression on the proliferation and migration of cancer cells. RESULTS: The expression of CDCA5 protein in (MDA-ME-231) and (BT549) cells in control group was significantly higher than that in negative control group (P < 0.05). Compared with the control group, the inhibition rates of CDCA5 expression in 1(MDA-ME-231), 2(MDA-ME-231), 1(BT549), and 2(BT549) cells in the interference group were 39.01%, 42.98%, 49.57%, and 60.98%, respectively (P < 0.05). From 12 h, the proliferation level of TNBC cells at different culture time was lower than that of the control group (P < 0.05). Compared with the number of staining cells in the control group, the positive staining cells in 1(MDA-ME-231) (61.42%), 2(MDA-ME-231) (72.06%), 1(BT549) (52.53%), and 2(BT549) (59.65%) in the interference group were significantly decreased (P < 0.05). CONCLUSION: The results show that the expression of CDCA5 in TNBC is increased, which plays an important role in the proliferation and migration of cancer cells. shRNA interference technology can knock down the expression of CDCA5 and inhibit its “promoting cancer” effect.