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Insight Into the Properties and Immunoregulatory Effect of Extracellular Vesicles Produced by Candida glabrata, Candida parapsilosis, and Candida tropicalis Biofilms

Currently, non-albicans Candida species, including C. tropicalis, C. glabrata, and C. parapsilosis, are becoming an increasing epidemiological threat, predominantly due to the distinct collection of virulence mechanisms, as well as emerging resistance to antifungal drugs typically used in the treatm...

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Autores principales: Kulig, Kamila, Karnas, Elzbieta, Woznicka, Olga, Kuleta, Patryk, Zuba-Surma, Ewa, Pyza, Elzbieta, Osyczka, Artur, Kozik, Andrzej, Rapala-Kozik, Maria, Karkowska-Kuleta, Justyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207348/
https://www.ncbi.nlm.nih.gov/pubmed/35734578
http://dx.doi.org/10.3389/fcimb.2022.879237
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author Kulig, Kamila
Karnas, Elzbieta
Woznicka, Olga
Kuleta, Patryk
Zuba-Surma, Ewa
Pyza, Elzbieta
Osyczka, Artur
Kozik, Andrzej
Rapala-Kozik, Maria
Karkowska-Kuleta, Justyna
author_facet Kulig, Kamila
Karnas, Elzbieta
Woznicka, Olga
Kuleta, Patryk
Zuba-Surma, Ewa
Pyza, Elzbieta
Osyczka, Artur
Kozik, Andrzej
Rapala-Kozik, Maria
Karkowska-Kuleta, Justyna
author_sort Kulig, Kamila
collection PubMed
description Currently, non-albicans Candida species, including C. tropicalis, C. glabrata, and C. parapsilosis, are becoming an increasing epidemiological threat, predominantly due to the distinct collection of virulence mechanisms, as well as emerging resistance to antifungal drugs typically used in the treatment of candidiasis. They can produce biofilms that release extracellular vesicles (EVs), which are nanometric spherical structures surrounded by a lipid bilayer, transporting diversified biologically active cargo, that may be involved in intercellular communication, biofilm matrix production, and interaction with the host. In this work, we characterize the size and protein composition of these structures for three species of non-albicans Candida fungi forming biofilm, indicating considerable heterogeneity of the investigated population of fungal EVs. Examination of the influence of EVs on cytokine production by the human monocytic cell line THP-1 differentiated into macrophage-like cells revealed that the tested vesicles have a stimulating effect on the secretion of tumor necrosis factor α and interleukin 8, while they reduce the production of interleukin 10. This may indicate the proinflammatory nature of the effect of EVs produced by these species on the host immune cells. Moreover, it has been indicated that vesicles may be involved in C. tropicalis biofilm resistance to fluconazole and caspofungin. This reveals the important role of EVs not only in the physiology of C. tropicalis, C. glabrata, and C. parapsilosis fungi but also in the pathogenesis of infections associated with the production of fungal biofilm.
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spelling pubmed-92073482022-06-21 Insight Into the Properties and Immunoregulatory Effect of Extracellular Vesicles Produced by Candida glabrata, Candida parapsilosis, and Candida tropicalis Biofilms Kulig, Kamila Karnas, Elzbieta Woznicka, Olga Kuleta, Patryk Zuba-Surma, Ewa Pyza, Elzbieta Osyczka, Artur Kozik, Andrzej Rapala-Kozik, Maria Karkowska-Kuleta, Justyna Front Cell Infect Microbiol Cellular and Infection Microbiology Currently, non-albicans Candida species, including C. tropicalis, C. glabrata, and C. parapsilosis, are becoming an increasing epidemiological threat, predominantly due to the distinct collection of virulence mechanisms, as well as emerging resistance to antifungal drugs typically used in the treatment of candidiasis. They can produce biofilms that release extracellular vesicles (EVs), which are nanometric spherical structures surrounded by a lipid bilayer, transporting diversified biologically active cargo, that may be involved in intercellular communication, biofilm matrix production, and interaction with the host. In this work, we characterize the size and protein composition of these structures for three species of non-albicans Candida fungi forming biofilm, indicating considerable heterogeneity of the investigated population of fungal EVs. Examination of the influence of EVs on cytokine production by the human monocytic cell line THP-1 differentiated into macrophage-like cells revealed that the tested vesicles have a stimulating effect on the secretion of tumor necrosis factor α and interleukin 8, while they reduce the production of interleukin 10. This may indicate the proinflammatory nature of the effect of EVs produced by these species on the host immune cells. Moreover, it has been indicated that vesicles may be involved in C. tropicalis biofilm resistance to fluconazole and caspofungin. This reveals the important role of EVs not only in the physiology of C. tropicalis, C. glabrata, and C. parapsilosis fungi but also in the pathogenesis of infections associated with the production of fungal biofilm. Frontiers Media S.A. 2022-06-06 /pmc/articles/PMC9207348/ /pubmed/35734578 http://dx.doi.org/10.3389/fcimb.2022.879237 Text en Copyright © 2022 Kulig, Karnas, Woznicka, Kuleta, Zuba-Surma, Pyza, Osyczka, Kozik, Rapala-Kozik and Karkowska-Kuleta https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Kulig, Kamila
Karnas, Elzbieta
Woznicka, Olga
Kuleta, Patryk
Zuba-Surma, Ewa
Pyza, Elzbieta
Osyczka, Artur
Kozik, Andrzej
Rapala-Kozik, Maria
Karkowska-Kuleta, Justyna
Insight Into the Properties and Immunoregulatory Effect of Extracellular Vesicles Produced by Candida glabrata, Candida parapsilosis, and Candida tropicalis Biofilms
title Insight Into the Properties and Immunoregulatory Effect of Extracellular Vesicles Produced by Candida glabrata, Candida parapsilosis, and Candida tropicalis Biofilms
title_full Insight Into the Properties and Immunoregulatory Effect of Extracellular Vesicles Produced by Candida glabrata, Candida parapsilosis, and Candida tropicalis Biofilms
title_fullStr Insight Into the Properties and Immunoregulatory Effect of Extracellular Vesicles Produced by Candida glabrata, Candida parapsilosis, and Candida tropicalis Biofilms
title_full_unstemmed Insight Into the Properties and Immunoregulatory Effect of Extracellular Vesicles Produced by Candida glabrata, Candida parapsilosis, and Candida tropicalis Biofilms
title_short Insight Into the Properties and Immunoregulatory Effect of Extracellular Vesicles Produced by Candida glabrata, Candida parapsilosis, and Candida tropicalis Biofilms
title_sort insight into the properties and immunoregulatory effect of extracellular vesicles produced by candida glabrata, candida parapsilosis, and candida tropicalis biofilms
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207348/
https://www.ncbi.nlm.nih.gov/pubmed/35734578
http://dx.doi.org/10.3389/fcimb.2022.879237
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