Cargando…
PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer
Ovarian cancer is the fifth most common cause of cancer‐related death in women. Ovarian clear cell carcinoma (OCCC) is a chemotherapy‐resistant epithelial ovarian cancer with poor prognosis. As a basis for the development of therapeutic agents that could improve the prognosis of OCCC, we performed a...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207365/ https://www.ncbi.nlm.nih.gov/pubmed/35377528 http://dx.doi.org/10.1111/cas.15358 |
_version_ | 1784729512331706368 |
---|---|
author | Akasu‐Nagayoshi, Yoko Hayashi, Tomoatsu Kawabata, Ayako Shimizu, Naomi Yamada, Ai Yokota, Naoko Nakato, Ryuichiro Shirahige, Katsuhiko Okamoto, Aikou Akiyama, Tetsu |
author_facet | Akasu‐Nagayoshi, Yoko Hayashi, Tomoatsu Kawabata, Ayako Shimizu, Naomi Yamada, Ai Yokota, Naoko Nakato, Ryuichiro Shirahige, Katsuhiko Okamoto, Aikou Akiyama, Tetsu |
author_sort | Akasu‐Nagayoshi, Yoko |
collection | PubMed |
description | Ovarian cancer is the fifth most common cause of cancer‐related death in women. Ovarian clear cell carcinoma (OCCC) is a chemotherapy‐resistant epithelial ovarian cancer with poor prognosis. As a basis for the development of therapeutic agents that could improve the prognosis of OCCC, we performed a screen for proteins critical for the tumorigenicity of OCCC using the CRISPR/Cas9 system. Here we show that knockdown of the phosphate exporter XPR1/SLC53A1 induces the growth arrest and apoptosis of OCCC cells in vitro. Moreover, we show that knockdown of XPR1/SLC53A1 inhibits the proliferation of OCCC cells xenografted into immunocompromised mice. These results suggest that XPR1/SLC53A1 plays a critical role in the tumorigenesis of OCCC cells. We speculate that XPR1/SLC53A1 might be a promising molecular target for the therapeutic treatment of OCCC. |
format | Online Article Text |
id | pubmed-9207365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92073652022-06-27 PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer Akasu‐Nagayoshi, Yoko Hayashi, Tomoatsu Kawabata, Ayako Shimizu, Naomi Yamada, Ai Yokota, Naoko Nakato, Ryuichiro Shirahige, Katsuhiko Okamoto, Aikou Akiyama, Tetsu Cancer Sci ORIGINAL ARTICLES Ovarian cancer is the fifth most common cause of cancer‐related death in women. Ovarian clear cell carcinoma (OCCC) is a chemotherapy‐resistant epithelial ovarian cancer with poor prognosis. As a basis for the development of therapeutic agents that could improve the prognosis of OCCC, we performed a screen for proteins critical for the tumorigenicity of OCCC using the CRISPR/Cas9 system. Here we show that knockdown of the phosphate exporter XPR1/SLC53A1 induces the growth arrest and apoptosis of OCCC cells in vitro. Moreover, we show that knockdown of XPR1/SLC53A1 inhibits the proliferation of OCCC cells xenografted into immunocompromised mice. These results suggest that XPR1/SLC53A1 plays a critical role in the tumorigenesis of OCCC cells. We speculate that XPR1/SLC53A1 might be a promising molecular target for the therapeutic treatment of OCCC. John Wiley and Sons Inc. 2022-04-24 2022-06 /pmc/articles/PMC9207365/ /pubmed/35377528 http://dx.doi.org/10.1111/cas.15358 Text en © 2022 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | ORIGINAL ARTICLES Akasu‐Nagayoshi, Yoko Hayashi, Tomoatsu Kawabata, Ayako Shimizu, Naomi Yamada, Ai Yokota, Naoko Nakato, Ryuichiro Shirahige, Katsuhiko Okamoto, Aikou Akiyama, Tetsu PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer |
title | PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer |
title_full | PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer |
title_fullStr | PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer |
title_full_unstemmed | PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer |
title_short | PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer |
title_sort | phosphate exporter xpr1/slc53a1 is required for the tumorigenicity of epithelial ovarian cancer |
topic | ORIGINAL ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207365/ https://www.ncbi.nlm.nih.gov/pubmed/35377528 http://dx.doi.org/10.1111/cas.15358 |
work_keys_str_mv | AT akasunagayoshiyoko phosphateexporterxpr1slc53a1isrequiredforthetumorigenicityofepithelialovariancancer AT hayashitomoatsu phosphateexporterxpr1slc53a1isrequiredforthetumorigenicityofepithelialovariancancer AT kawabataayako phosphateexporterxpr1slc53a1isrequiredforthetumorigenicityofepithelialovariancancer AT shimizunaomi phosphateexporterxpr1slc53a1isrequiredforthetumorigenicityofepithelialovariancancer AT yamadaai phosphateexporterxpr1slc53a1isrequiredforthetumorigenicityofepithelialovariancancer AT yokotanaoko phosphateexporterxpr1slc53a1isrequiredforthetumorigenicityofepithelialovariancancer AT nakatoryuichiro phosphateexporterxpr1slc53a1isrequiredforthetumorigenicityofepithelialovariancancer AT shirahigekatsuhiko phosphateexporterxpr1slc53a1isrequiredforthetumorigenicityofepithelialovariancancer AT okamotoaikou phosphateexporterxpr1slc53a1isrequiredforthetumorigenicityofepithelialovariancancer AT akiyamatetsu phosphateexporterxpr1slc53a1isrequiredforthetumorigenicityofepithelialovariancancer |