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PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer

Ovarian cancer is the fifth most common cause of cancer‐related death in women. Ovarian clear cell carcinoma (OCCC) is a chemotherapy‐resistant epithelial ovarian cancer with poor prognosis. As a basis for the development of therapeutic agents that could improve the prognosis of OCCC, we performed a...

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Autores principales: Akasu‐Nagayoshi, Yoko, Hayashi, Tomoatsu, Kawabata, Ayako, Shimizu, Naomi, Yamada, Ai, Yokota, Naoko, Nakato, Ryuichiro, Shirahige, Katsuhiko, Okamoto, Aikou, Akiyama, Tetsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207365/
https://www.ncbi.nlm.nih.gov/pubmed/35377528
http://dx.doi.org/10.1111/cas.15358
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author Akasu‐Nagayoshi, Yoko
Hayashi, Tomoatsu
Kawabata, Ayako
Shimizu, Naomi
Yamada, Ai
Yokota, Naoko
Nakato, Ryuichiro
Shirahige, Katsuhiko
Okamoto, Aikou
Akiyama, Tetsu
author_facet Akasu‐Nagayoshi, Yoko
Hayashi, Tomoatsu
Kawabata, Ayako
Shimizu, Naomi
Yamada, Ai
Yokota, Naoko
Nakato, Ryuichiro
Shirahige, Katsuhiko
Okamoto, Aikou
Akiyama, Tetsu
author_sort Akasu‐Nagayoshi, Yoko
collection PubMed
description Ovarian cancer is the fifth most common cause of cancer‐related death in women. Ovarian clear cell carcinoma (OCCC) is a chemotherapy‐resistant epithelial ovarian cancer with poor prognosis. As a basis for the development of therapeutic agents that could improve the prognosis of OCCC, we performed a screen for proteins critical for the tumorigenicity of OCCC using the CRISPR/Cas9 system. Here we show that knockdown of the phosphate exporter XPR1/SLC53A1 induces the growth arrest and apoptosis of OCCC cells in vitro. Moreover, we show that knockdown of XPR1/SLC53A1 inhibits the proliferation of OCCC cells xenografted into immunocompromised mice. These results suggest that XPR1/SLC53A1 plays a critical role in the tumorigenesis of OCCC cells. We speculate that XPR1/SLC53A1 might be a promising molecular target for the therapeutic treatment of OCCC.
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spelling pubmed-92073652022-06-27 PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer Akasu‐Nagayoshi, Yoko Hayashi, Tomoatsu Kawabata, Ayako Shimizu, Naomi Yamada, Ai Yokota, Naoko Nakato, Ryuichiro Shirahige, Katsuhiko Okamoto, Aikou Akiyama, Tetsu Cancer Sci ORIGINAL ARTICLES Ovarian cancer is the fifth most common cause of cancer‐related death in women. Ovarian clear cell carcinoma (OCCC) is a chemotherapy‐resistant epithelial ovarian cancer with poor prognosis. As a basis for the development of therapeutic agents that could improve the prognosis of OCCC, we performed a screen for proteins critical for the tumorigenicity of OCCC using the CRISPR/Cas9 system. Here we show that knockdown of the phosphate exporter XPR1/SLC53A1 induces the growth arrest and apoptosis of OCCC cells in vitro. Moreover, we show that knockdown of XPR1/SLC53A1 inhibits the proliferation of OCCC cells xenografted into immunocompromised mice. These results suggest that XPR1/SLC53A1 plays a critical role in the tumorigenesis of OCCC cells. We speculate that XPR1/SLC53A1 might be a promising molecular target for the therapeutic treatment of OCCC. John Wiley and Sons Inc. 2022-04-24 2022-06 /pmc/articles/PMC9207365/ /pubmed/35377528 http://dx.doi.org/10.1111/cas.15358 Text en © 2022 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle ORIGINAL ARTICLES
Akasu‐Nagayoshi, Yoko
Hayashi, Tomoatsu
Kawabata, Ayako
Shimizu, Naomi
Yamada, Ai
Yokota, Naoko
Nakato, Ryuichiro
Shirahige, Katsuhiko
Okamoto, Aikou
Akiyama, Tetsu
PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer
title PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer
title_full PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer
title_fullStr PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer
title_full_unstemmed PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer
title_short PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer
title_sort phosphate exporter xpr1/slc53a1 is required for the tumorigenicity of epithelial ovarian cancer
topic ORIGINAL ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207365/
https://www.ncbi.nlm.nih.gov/pubmed/35377528
http://dx.doi.org/10.1111/cas.15358
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