Cargando…

circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

Circular RNA (circRNA) participates in a variety of pathophysiological processes, including the development of gastric cancer (GC). However, the role of circ_0006089 in GC progression and its underlying molecular mechanism need to be further revealed. Quantitative real‐time PCR was utilized for dete...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Ying, Zhang, Qilin, Liao, Bingling, Qiu, Xiaofeng, Hu, Sheng, Xu, Qihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207367/
https://www.ncbi.nlm.nih.gov/pubmed/35347818
http://dx.doi.org/10.1111/cas.15351
_version_ 1784729513578463232
author Zhou, Ying
Zhang, Qilin
Liao, Bingling
Qiu, Xiaofeng
Hu, Sheng
Xu, Qihua
author_facet Zhou, Ying
Zhang, Qilin
Liao, Bingling
Qiu, Xiaofeng
Hu, Sheng
Xu, Qihua
author_sort Zhou, Ying
collection PubMed
description Circular RNA (circRNA) participates in a variety of pathophysiological processes, including the development of gastric cancer (GC). However, the role of circ_0006089 in GC progression and its underlying molecular mechanism need to be further revealed. Quantitative real‐time PCR was utilized for detecting circ_0006089, microRNA (miR)‐361‐3p and transforming growth factor‐β1 (TGFB1) expression. The interaction between miR‐361‐3p and circ_0006089 or TGFB1 was confirmed using a dual‐luciferase reporter assay and an RNA immunoprecipitation (RIP) assay. Cell proliferation, metastasis, apoptosis, and angiogenesis were determined using colony formation assay, EdU assay, transwell assay, flow cytometry, and tube formation assay. Cell glycolysis was evaluated by detecting glucose consumption, lactate production, and ATP levels. In addition, western blot (WB) analysis was used to measure protein expression. Xenograft tumor models were used to assess the effect of circ_0006089 knockdown on GC tumorigenesis. circ_0006089 had been found to be upregulated in GC tissues and cells, and it could act as an miR‐361‐3p sponge. circ_0006089 knockdown suppressed GC proliferation, metastasis, glycolysis, angiogenesis, and increased apoptosis, while this effect could be revoked by miR‐361‐3p inhibitor. TGFB1 was targeted by miR‐361‐3p, and its overexpression reversed the effects of miR‐361‐3p on GC cell function. Also, circ_0006089 promoted TGFB1 expression via sponging miR‐361‐3p. Animal experiments showed that silenced circ_0006089 inhibited GC tumorigenesis through the miR‐361‐3p/TGFB1 pathway. Our results revealed that the circ_0006089/miR‐361‐3p/TGFB1 axis contributed to GC progression, confirming that circ_0006089 might be a potential therapeutic target for GC.
format Online
Article
Text
id pubmed-9207367
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-92073672022-06-27 circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1 Zhou, Ying Zhang, Qilin Liao, Bingling Qiu, Xiaofeng Hu, Sheng Xu, Qihua Cancer Sci ORIGINAL ARTICLES Circular RNA (circRNA) participates in a variety of pathophysiological processes, including the development of gastric cancer (GC). However, the role of circ_0006089 in GC progression and its underlying molecular mechanism need to be further revealed. Quantitative real‐time PCR was utilized for detecting circ_0006089, microRNA (miR)‐361‐3p and transforming growth factor‐β1 (TGFB1) expression. The interaction between miR‐361‐3p and circ_0006089 or TGFB1 was confirmed using a dual‐luciferase reporter assay and an RNA immunoprecipitation (RIP) assay. Cell proliferation, metastasis, apoptosis, and angiogenesis were determined using colony formation assay, EdU assay, transwell assay, flow cytometry, and tube formation assay. Cell glycolysis was evaluated by detecting glucose consumption, lactate production, and ATP levels. In addition, western blot (WB) analysis was used to measure protein expression. Xenograft tumor models were used to assess the effect of circ_0006089 knockdown on GC tumorigenesis. circ_0006089 had been found to be upregulated in GC tissues and cells, and it could act as an miR‐361‐3p sponge. circ_0006089 knockdown suppressed GC proliferation, metastasis, glycolysis, angiogenesis, and increased apoptosis, while this effect could be revoked by miR‐361‐3p inhibitor. TGFB1 was targeted by miR‐361‐3p, and its overexpression reversed the effects of miR‐361‐3p on GC cell function. Also, circ_0006089 promoted TGFB1 expression via sponging miR‐361‐3p. Animal experiments showed that silenced circ_0006089 inhibited GC tumorigenesis through the miR‐361‐3p/TGFB1 pathway. Our results revealed that the circ_0006089/miR‐361‐3p/TGFB1 axis contributed to GC progression, confirming that circ_0006089 might be a potential therapeutic target for GC. John Wiley and Sons Inc. 2022-04-24 2022-06 /pmc/articles/PMC9207367/ /pubmed/35347818 http://dx.doi.org/10.1111/cas.15351 Text en © 2022 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle ORIGINAL ARTICLES
Zhou, Ying
Zhang, Qilin
Liao, Bingling
Qiu, Xiaofeng
Hu, Sheng
Xu, Qihua
circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1
title circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1
title_full circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1
title_fullStr circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1
title_full_unstemmed circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1
title_short circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1
title_sort circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating mir‐361‐3p/tgfb1
topic ORIGINAL ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207367/
https://www.ncbi.nlm.nih.gov/pubmed/35347818
http://dx.doi.org/10.1111/cas.15351
work_keys_str_mv AT zhouying circ0006089promotesgastriccancergrowthmetastasisglycolysisandangiogenesisbyregulatingmir3613ptgfb1
AT zhangqilin circ0006089promotesgastriccancergrowthmetastasisglycolysisandangiogenesisbyregulatingmir3613ptgfb1
AT liaobingling circ0006089promotesgastriccancergrowthmetastasisglycolysisandangiogenesisbyregulatingmir3613ptgfb1
AT qiuxiaofeng circ0006089promotesgastriccancergrowthmetastasisglycolysisandangiogenesisbyregulatingmir3613ptgfb1
AT husheng circ0006089promotesgastriccancergrowthmetastasisglycolysisandangiogenesisbyregulatingmir3613ptgfb1
AT xuqihua circ0006089promotesgastriccancergrowthmetastasisglycolysisandangiogenesisbyregulatingmir3613ptgfb1