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Cannabidiol Reduces Inflammatory Lung Damage After Meconium Aspiration in Newborn Piglets
AIM: To assess the effects of cannabidiol (CBD) on lung damage in a piglet model of meconium aspiration syndrome (MAS). MATERIALS AND METHODS: Meconium aspiration syndrome was modelled in newborn piglets via intratracheal instillation of 20% meconium in saline collected from healthy newborn humans....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207394/ https://www.ncbi.nlm.nih.gov/pubmed/35733813 http://dx.doi.org/10.3389/fped.2022.862035 |
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author | Arruza, Luis Barata, Lorena Vierge, Eva Rodríguez, Maria José Del Pozo, Aaron Hind, William Martínez-Orgado, José |
author_facet | Arruza, Luis Barata, Lorena Vierge, Eva Rodríguez, Maria José Del Pozo, Aaron Hind, William Martínez-Orgado, José |
author_sort | Arruza, Luis |
collection | PubMed |
description | AIM: To assess the effects of cannabidiol (CBD) on lung damage in a piglet model of meconium aspiration syndrome (MAS). MATERIALS AND METHODS: Meconium aspiration syndrome was modelled in newborn piglets via intratracheal instillation of 20% meconium in saline collected from healthy newborn humans. Piglets were treated i.v. with 5 mg/kg CBD (MAS + CBD) or Vehicle (MAS + VEH) 30 min after MAS induction and monitored for 6 h. Ventilated piglets without meconium instillation served as controls (CTL). Ventilatory and haemodynamic monitoring, histological and biochemical studies assessed the effects of treatment. RESULTS: Post-insult administration of CBD reduced MAS-induced deterioration of gas exchange, improving respiratory acidosis (final pH 7.38 ± 0.02, 7.22 ± 0.03 and 7.33 ± 0.03 and final pCO(2) 39.8 ± 1.3, 60.4 ± 3.8 and 45.7 ± 3.1 mmHg for CTL, MAS + VEH and MAS + CBD, respectively, p < 0.05). These beneficial effects were obtained despite the less aggressive ventilatory settings required for CBD-treated animals (final minute volume 230 ± 30, 348 ± 33 and 253 ± 24 mL/kg/min and final Oxygenation Index 1.64 ± 0.04, 12.57 ± 3.10 and 7.42 ± 2.07 mmHg for CTL, MAS + VEH and MAS + CBD, respectively, p < 0.05). CBD’s beneficial effects on gas exchange were associated with reduced histological lung damage, reduced leucocyte infiltration and oedema (histopathological score 1.6 ± 0.3, 8.6 ± 1.4 and 4.6 ± 0.7 points for CTL, MAS + VEH and MAS + CBD, respectively, p < 0.05), as well as reduced TNFα production (0.04 ± 0.01, 0.34 ± 0.06 and 0.12 ± 0.02 A.U. for CTL, MAS + VEH and MAS + CBD, respectively, p < 0.05). Moreover, CBD improved blood pressure stability (final mean blood pressure 74.5 ± 0.2, 62.2 ± 6.2, and 78.67 ± 4.1 mmHg for CTL, MAS + VEH and MAS + CBD, respectively, p < 0.05). CONCLUSION: Cannabidiol reduces histologic lung damage and inflammation in a piglet model of MAS. This translates into improved gas exchange and blood pressure stability. |
format | Online Article Text |
id | pubmed-9207394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92073942022-06-21 Cannabidiol Reduces Inflammatory Lung Damage After Meconium Aspiration in Newborn Piglets Arruza, Luis Barata, Lorena Vierge, Eva Rodríguez, Maria José Del Pozo, Aaron Hind, William Martínez-Orgado, José Front Pediatr Pediatrics AIM: To assess the effects of cannabidiol (CBD) on lung damage in a piglet model of meconium aspiration syndrome (MAS). MATERIALS AND METHODS: Meconium aspiration syndrome was modelled in newborn piglets via intratracheal instillation of 20% meconium in saline collected from healthy newborn humans. Piglets were treated i.v. with 5 mg/kg CBD (MAS + CBD) or Vehicle (MAS + VEH) 30 min after MAS induction and monitored for 6 h. Ventilated piglets without meconium instillation served as controls (CTL). Ventilatory and haemodynamic monitoring, histological and biochemical studies assessed the effects of treatment. RESULTS: Post-insult administration of CBD reduced MAS-induced deterioration of gas exchange, improving respiratory acidosis (final pH 7.38 ± 0.02, 7.22 ± 0.03 and 7.33 ± 0.03 and final pCO(2) 39.8 ± 1.3, 60.4 ± 3.8 and 45.7 ± 3.1 mmHg for CTL, MAS + VEH and MAS + CBD, respectively, p < 0.05). These beneficial effects were obtained despite the less aggressive ventilatory settings required for CBD-treated animals (final minute volume 230 ± 30, 348 ± 33 and 253 ± 24 mL/kg/min and final Oxygenation Index 1.64 ± 0.04, 12.57 ± 3.10 and 7.42 ± 2.07 mmHg for CTL, MAS + VEH and MAS + CBD, respectively, p < 0.05). CBD’s beneficial effects on gas exchange were associated with reduced histological lung damage, reduced leucocyte infiltration and oedema (histopathological score 1.6 ± 0.3, 8.6 ± 1.4 and 4.6 ± 0.7 points for CTL, MAS + VEH and MAS + CBD, respectively, p < 0.05), as well as reduced TNFα production (0.04 ± 0.01, 0.34 ± 0.06 and 0.12 ± 0.02 A.U. for CTL, MAS + VEH and MAS + CBD, respectively, p < 0.05). Moreover, CBD improved blood pressure stability (final mean blood pressure 74.5 ± 0.2, 62.2 ± 6.2, and 78.67 ± 4.1 mmHg for CTL, MAS + VEH and MAS + CBD, respectively, p < 0.05). CONCLUSION: Cannabidiol reduces histologic lung damage and inflammation in a piglet model of MAS. This translates into improved gas exchange and blood pressure stability. Frontiers Media S.A. 2022-06-06 /pmc/articles/PMC9207394/ /pubmed/35733813 http://dx.doi.org/10.3389/fped.2022.862035 Text en Copyright © 2022 Arruza, Barata, Vierge, Rodríguez, Del Pozo, Hind and Martínez-Orgado. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pediatrics Arruza, Luis Barata, Lorena Vierge, Eva Rodríguez, Maria José Del Pozo, Aaron Hind, William Martínez-Orgado, José Cannabidiol Reduces Inflammatory Lung Damage After Meconium Aspiration in Newborn Piglets |
title | Cannabidiol Reduces Inflammatory Lung Damage After Meconium Aspiration in Newborn Piglets |
title_full | Cannabidiol Reduces Inflammatory Lung Damage After Meconium Aspiration in Newborn Piglets |
title_fullStr | Cannabidiol Reduces Inflammatory Lung Damage After Meconium Aspiration in Newborn Piglets |
title_full_unstemmed | Cannabidiol Reduces Inflammatory Lung Damage After Meconium Aspiration in Newborn Piglets |
title_short | Cannabidiol Reduces Inflammatory Lung Damage After Meconium Aspiration in Newborn Piglets |
title_sort | cannabidiol reduces inflammatory lung damage after meconium aspiration in newborn piglets |
topic | Pediatrics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207394/ https://www.ncbi.nlm.nih.gov/pubmed/35733813 http://dx.doi.org/10.3389/fped.2022.862035 |
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