Cargando…

Molecular Structure and Phylogenetic Analyses of the Complete Chloroplast Genomes of Three Medicinal Plants Conioselinum vaginatum, Ligusticum sinense, and Ligusticum jeholense

Most plants of Ligusticum have an important medicinal and economic value with a long history, Ligusticum sinense and L. jeholense (“Gaoben”) has long been used in traditional Chinese medicine for the treatment of carminative, dispelling cold, dehumidification, and analgesia. While in the market Coni...

Descripción completa

Detalles Bibliográficos
Autores principales: Wei, Xue-Ping, Zhang, Xiao-Yi, Dong, Yu-Qing, Cheng, Ji-Long, Bai, Yun-Jun, Liu, Jiu-Shi, Qi, Yao-Dong, Zhang, Ben-Gang, Liu, Hai-Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207526/
https://www.ncbi.nlm.nih.gov/pubmed/35734262
http://dx.doi.org/10.3389/fpls.2022.878263
_version_ 1784729551278964736
author Wei, Xue-Ping
Zhang, Xiao-Yi
Dong, Yu-Qing
Cheng, Ji-Long
Bai, Yun-Jun
Liu, Jiu-Shi
Qi, Yao-Dong
Zhang, Ben-Gang
Liu, Hai-Tao
author_facet Wei, Xue-Ping
Zhang, Xiao-Yi
Dong, Yu-Qing
Cheng, Ji-Long
Bai, Yun-Jun
Liu, Jiu-Shi
Qi, Yao-Dong
Zhang, Ben-Gang
Liu, Hai-Tao
author_sort Wei, Xue-Ping
collection PubMed
description Most plants of Ligusticum have an important medicinal and economic value with a long history, Ligusticum sinense and L. jeholense (“Gaoben”) has long been used in traditional Chinese medicine for the treatment of carminative, dispelling cold, dehumidification, and analgesia. While in the market Conioselinum vaginatum (Xinjiang Gaoben) is substitution for Gaoben, and occupies a higher market share. These three Gaoben-related medicinal materials are similar in morphology, and are difficult to distinguish from each other by the commonly used DNA barcodes. The chloroplast genome has been widely used for molecular markers, evolutionary biology, and barcoding identification. In this study, the complete chloroplast genome sequences of C. vaginatum, L. sinense, and L. jeholense were reported. The results showed that the complete chloroplast genomes of these three species have typical quadripartite structures, which were comprised of 148,664, 148,539, and 148,497 bp. A total of 114 genes were identified, including 81 protein-coding genes (PCGs), 29 tRNA genes, and four rRNA genes. Our study indicated that highly variable region ycf2-trnL and accD-ycf4 that can be used as specific DNA barcodes to distinguish and identify C. vaginatum, L. sinense, and L. jeholense. In addition, phylogenetic study showed that C. vaginatum nested in Ligusticum and as a sister group of L. sinense and L. jeholense, which suggested these two genera are both in need of revision. This study offer valuable information for future research in the identification of Gaoben-related medicinal materials and will benefit for further phylogenetic study of Apiaceae.
format Online
Article
Text
id pubmed-9207526
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92075262022-06-21 Molecular Structure and Phylogenetic Analyses of the Complete Chloroplast Genomes of Three Medicinal Plants Conioselinum vaginatum, Ligusticum sinense, and Ligusticum jeholense Wei, Xue-Ping Zhang, Xiao-Yi Dong, Yu-Qing Cheng, Ji-Long Bai, Yun-Jun Liu, Jiu-Shi Qi, Yao-Dong Zhang, Ben-Gang Liu, Hai-Tao Front Plant Sci Plant Science Most plants of Ligusticum have an important medicinal and economic value with a long history, Ligusticum sinense and L. jeholense (“Gaoben”) has long been used in traditional Chinese medicine for the treatment of carminative, dispelling cold, dehumidification, and analgesia. While in the market Conioselinum vaginatum (Xinjiang Gaoben) is substitution for Gaoben, and occupies a higher market share. These three Gaoben-related medicinal materials are similar in morphology, and are difficult to distinguish from each other by the commonly used DNA barcodes. The chloroplast genome has been widely used for molecular markers, evolutionary biology, and barcoding identification. In this study, the complete chloroplast genome sequences of C. vaginatum, L. sinense, and L. jeholense were reported. The results showed that the complete chloroplast genomes of these three species have typical quadripartite structures, which were comprised of 148,664, 148,539, and 148,497 bp. A total of 114 genes were identified, including 81 protein-coding genes (PCGs), 29 tRNA genes, and four rRNA genes. Our study indicated that highly variable region ycf2-trnL and accD-ycf4 that can be used as specific DNA barcodes to distinguish and identify C. vaginatum, L. sinense, and L. jeholense. In addition, phylogenetic study showed that C. vaginatum nested in Ligusticum and as a sister group of L. sinense and L. jeholense, which suggested these two genera are both in need of revision. This study offer valuable information for future research in the identification of Gaoben-related medicinal materials and will benefit for further phylogenetic study of Apiaceae. Frontiers Media S.A. 2022-06-06 /pmc/articles/PMC9207526/ /pubmed/35734262 http://dx.doi.org/10.3389/fpls.2022.878263 Text en Copyright © 2022 Wei, Zhang, Dong, Cheng, Bai, Liu, Qi, Zhang and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wei, Xue-Ping
Zhang, Xiao-Yi
Dong, Yu-Qing
Cheng, Ji-Long
Bai, Yun-Jun
Liu, Jiu-Shi
Qi, Yao-Dong
Zhang, Ben-Gang
Liu, Hai-Tao
Molecular Structure and Phylogenetic Analyses of the Complete Chloroplast Genomes of Three Medicinal Plants Conioselinum vaginatum, Ligusticum sinense, and Ligusticum jeholense
title Molecular Structure and Phylogenetic Analyses of the Complete Chloroplast Genomes of Three Medicinal Plants Conioselinum vaginatum, Ligusticum sinense, and Ligusticum jeholense
title_full Molecular Structure and Phylogenetic Analyses of the Complete Chloroplast Genomes of Three Medicinal Plants Conioselinum vaginatum, Ligusticum sinense, and Ligusticum jeholense
title_fullStr Molecular Structure and Phylogenetic Analyses of the Complete Chloroplast Genomes of Three Medicinal Plants Conioselinum vaginatum, Ligusticum sinense, and Ligusticum jeholense
title_full_unstemmed Molecular Structure and Phylogenetic Analyses of the Complete Chloroplast Genomes of Three Medicinal Plants Conioselinum vaginatum, Ligusticum sinense, and Ligusticum jeholense
title_short Molecular Structure and Phylogenetic Analyses of the Complete Chloroplast Genomes of Three Medicinal Plants Conioselinum vaginatum, Ligusticum sinense, and Ligusticum jeholense
title_sort molecular structure and phylogenetic analyses of the complete chloroplast genomes of three medicinal plants conioselinum vaginatum, ligusticum sinense, and ligusticum jeholense
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207526/
https://www.ncbi.nlm.nih.gov/pubmed/35734262
http://dx.doi.org/10.3389/fpls.2022.878263
work_keys_str_mv AT weixueping molecularstructureandphylogeneticanalysesofthecompletechloroplastgenomesofthreemedicinalplantsconioselinumvaginatumligusticumsinenseandligusticumjeholense
AT zhangxiaoyi molecularstructureandphylogeneticanalysesofthecompletechloroplastgenomesofthreemedicinalplantsconioselinumvaginatumligusticumsinenseandligusticumjeholense
AT dongyuqing molecularstructureandphylogeneticanalysesofthecompletechloroplastgenomesofthreemedicinalplantsconioselinumvaginatumligusticumsinenseandligusticumjeholense
AT chengjilong molecularstructureandphylogeneticanalysesofthecompletechloroplastgenomesofthreemedicinalplantsconioselinumvaginatumligusticumsinenseandligusticumjeholense
AT baiyunjun molecularstructureandphylogeneticanalysesofthecompletechloroplastgenomesofthreemedicinalplantsconioselinumvaginatumligusticumsinenseandligusticumjeholense
AT liujiushi molecularstructureandphylogeneticanalysesofthecompletechloroplastgenomesofthreemedicinalplantsconioselinumvaginatumligusticumsinenseandligusticumjeholense
AT qiyaodong molecularstructureandphylogeneticanalysesofthecompletechloroplastgenomesofthreemedicinalplantsconioselinumvaginatumligusticumsinenseandligusticumjeholense
AT zhangbengang molecularstructureandphylogeneticanalysesofthecompletechloroplastgenomesofthreemedicinalplantsconioselinumvaginatumligusticumsinenseandligusticumjeholense
AT liuhaitao molecularstructureandphylogeneticanalysesofthecompletechloroplastgenomesofthreemedicinalplantsconioselinumvaginatumligusticumsinenseandligusticumjeholense