Cargando…

Indole-3-Carbinol (I3C) Protects the Heart From Ischemia/Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Cellular Apoptosis in Mice

Strategies for treating myocardial ischemia in the clinic usually include re-canalization of the coronary arteries to restore blood supply to the myocardium. However, myocardial reperfusion insult often leads to oxidative stress and inflammation, which in turn leads to apoptosis and necrosis of myoc...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Qi, Xia, Boyu, Wu, Jingjing, Yuan, Xiaomei, Lu, Xu, Huang, Chao, Gu, Hongcheng, Zheng, Koulong, You, Qingsheng, Liu, Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208008/
https://www.ncbi.nlm.nih.gov/pubmed/35734410
http://dx.doi.org/10.3389/fphar.2022.924174
_version_ 1784729647254077440
author Li, Qi
Xia, Boyu
Wu, Jingjing
Yuan, Xiaomei
Lu, Xu
Huang, Chao
Gu, Hongcheng
Zheng, Koulong
You, Qingsheng
Liu, Kun
author_facet Li, Qi
Xia, Boyu
Wu, Jingjing
Yuan, Xiaomei
Lu, Xu
Huang, Chao
Gu, Hongcheng
Zheng, Koulong
You, Qingsheng
Liu, Kun
author_sort Li, Qi
collection PubMed
description Strategies for treating myocardial ischemia in the clinic usually include re-canalization of the coronary arteries to restore blood supply to the myocardium. However, myocardial reperfusion insult often leads to oxidative stress and inflammation, which in turn leads to apoptosis and necrosis of myocardial cells, for which there are no standard treatment methods. The aim of this study was to determine the pharmacological effect of indole-3-carbinol (I3C), a phytochemical found in most cruciferous vegetables, in a mouse model of myocardial ischemia/reperfusion injury (MIRI). Our results showed that I3C pretreatment (100 mg/kg, once daily, i. p.) prevented the MIRI-induced increase in infarct size and serum creatine kinase (CK) and lactate dehydrogenase (LDH) in mice. I3C pretreatment also suppressed cardiac apoptosis in MIRI mice by increasing the expression levels of the anti-apoptotic protein Bcl-2 and decreasing the expression levels of several apoptotic proteins, including Bax, caspase-3, and caspase-9. In addition, I3C pretreatment was found to reduce the levels of parameters reflecting oxidative stress, such as dihydroethidium (DHE), malondialdehyde (MDA), reactive oxygen species (ROS), and nitric oxide (NO), while increasing the levels of parameters reflecting anti-oxidation, such as total antioxidant capacity (T-AOC) and glutathione (GSH), in MIRI-induced ischemic heart tissue. I3C pretreatment was also able to remarkably decrease the expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) mRNA in ischemic heart tissue. These results demonstrate that administration of I3C protects the heart from MIRI through its anti-apoptotic, antioxidant, and anti-inflammatory effects.
format Online
Article
Text
id pubmed-9208008
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92080082022-06-21 Indole-3-Carbinol (I3C) Protects the Heart From Ischemia/Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Cellular Apoptosis in Mice Li, Qi Xia, Boyu Wu, Jingjing Yuan, Xiaomei Lu, Xu Huang, Chao Gu, Hongcheng Zheng, Koulong You, Qingsheng Liu, Kun Front Pharmacol Pharmacology Strategies for treating myocardial ischemia in the clinic usually include re-canalization of the coronary arteries to restore blood supply to the myocardium. However, myocardial reperfusion insult often leads to oxidative stress and inflammation, which in turn leads to apoptosis and necrosis of myocardial cells, for which there are no standard treatment methods. The aim of this study was to determine the pharmacological effect of indole-3-carbinol (I3C), a phytochemical found in most cruciferous vegetables, in a mouse model of myocardial ischemia/reperfusion injury (MIRI). Our results showed that I3C pretreatment (100 mg/kg, once daily, i. p.) prevented the MIRI-induced increase in infarct size and serum creatine kinase (CK) and lactate dehydrogenase (LDH) in mice. I3C pretreatment also suppressed cardiac apoptosis in MIRI mice by increasing the expression levels of the anti-apoptotic protein Bcl-2 and decreasing the expression levels of several apoptotic proteins, including Bax, caspase-3, and caspase-9. In addition, I3C pretreatment was found to reduce the levels of parameters reflecting oxidative stress, such as dihydroethidium (DHE), malondialdehyde (MDA), reactive oxygen species (ROS), and nitric oxide (NO), while increasing the levels of parameters reflecting anti-oxidation, such as total antioxidant capacity (T-AOC) and glutathione (GSH), in MIRI-induced ischemic heart tissue. I3C pretreatment was also able to remarkably decrease the expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) mRNA in ischemic heart tissue. These results demonstrate that administration of I3C protects the heart from MIRI through its anti-apoptotic, antioxidant, and anti-inflammatory effects. Frontiers Media S.A. 2022-06-06 /pmc/articles/PMC9208008/ /pubmed/35734410 http://dx.doi.org/10.3389/fphar.2022.924174 Text en Copyright © 2022 Li, Xia, Wu, Yuan, Lu, Huang, Gu, Zheng, You and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Li, Qi
Xia, Boyu
Wu, Jingjing
Yuan, Xiaomei
Lu, Xu
Huang, Chao
Gu, Hongcheng
Zheng, Koulong
You, Qingsheng
Liu, Kun
Indole-3-Carbinol (I3C) Protects the Heart From Ischemia/Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Cellular Apoptosis in Mice
title Indole-3-Carbinol (I3C) Protects the Heart From Ischemia/Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Cellular Apoptosis in Mice
title_full Indole-3-Carbinol (I3C) Protects the Heart From Ischemia/Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Cellular Apoptosis in Mice
title_fullStr Indole-3-Carbinol (I3C) Protects the Heart From Ischemia/Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Cellular Apoptosis in Mice
title_full_unstemmed Indole-3-Carbinol (I3C) Protects the Heart From Ischemia/Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Cellular Apoptosis in Mice
title_short Indole-3-Carbinol (I3C) Protects the Heart From Ischemia/Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Cellular Apoptosis in Mice
title_sort indole-3-carbinol (i3c) protects the heart from ischemia/reperfusion injury by inhibiting oxidative stress, inflammation, and cellular apoptosis in mice
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208008/
https://www.ncbi.nlm.nih.gov/pubmed/35734410
http://dx.doi.org/10.3389/fphar.2022.924174
work_keys_str_mv AT liqi indole3carbinoli3cprotectstheheartfromischemiareperfusioninjurybyinhibitingoxidativestressinflammationandcellularapoptosisinmice
AT xiaboyu indole3carbinoli3cprotectstheheartfromischemiareperfusioninjurybyinhibitingoxidativestressinflammationandcellularapoptosisinmice
AT wujingjing indole3carbinoli3cprotectstheheartfromischemiareperfusioninjurybyinhibitingoxidativestressinflammationandcellularapoptosisinmice
AT yuanxiaomei indole3carbinoli3cprotectstheheartfromischemiareperfusioninjurybyinhibitingoxidativestressinflammationandcellularapoptosisinmice
AT luxu indole3carbinoli3cprotectstheheartfromischemiareperfusioninjurybyinhibitingoxidativestressinflammationandcellularapoptosisinmice
AT huangchao indole3carbinoli3cprotectstheheartfromischemiareperfusioninjurybyinhibitingoxidativestressinflammationandcellularapoptosisinmice
AT guhongcheng indole3carbinoli3cprotectstheheartfromischemiareperfusioninjurybyinhibitingoxidativestressinflammationandcellularapoptosisinmice
AT zhengkoulong indole3carbinoli3cprotectstheheartfromischemiareperfusioninjurybyinhibitingoxidativestressinflammationandcellularapoptosisinmice
AT youqingsheng indole3carbinoli3cprotectstheheartfromischemiareperfusioninjurybyinhibitingoxidativestressinflammationandcellularapoptosisinmice
AT liukun indole3carbinoli3cprotectstheheartfromischemiareperfusioninjurybyinhibitingoxidativestressinflammationandcellularapoptosisinmice