Cargando…
The Developmental Delay of Seedlings With Cotyledons Only Confers Stress Tolerance to Suaeda aralocaspica (Chenopodiaceae) by Unique Performance on Morphology, Physiology, and Gene Expression
Cotyledons play an important role in seedling establishment, although they may just exist for a short time and become senescent upon the emergence of euphylla. So far, the detailed function of cotyledons has not been well understood. Suaeda aralocaspica is an annual halophyte distributed in cold des...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208309/ https://www.ncbi.nlm.nih.gov/pubmed/35734249 http://dx.doi.org/10.3389/fpls.2022.844430 |
_version_ | 1784729714679611392 |
---|---|
author | Cao, Jing Li, Xiaorong Chen, Ling He, Meixiang Lan, Haiyan |
author_facet | Cao, Jing Li, Xiaorong Chen, Ling He, Meixiang Lan, Haiyan |
author_sort | Cao, Jing |
collection | PubMed |
description | Cotyledons play an important role in seedling establishment, although they may just exist for a short time and become senescent upon the emergence of euphylla. So far, the detailed function of cotyledons has not been well understood. Suaeda aralocaspica is an annual halophyte distributed in cold deserts; its cotyledons could exist for a longer time, even last until maturity, and they must exert a unique function in seedling development. Therefore, in this study, we conducted a series of experiments to investigate the morphological and physiological performances of cotyledons under salt stress at different developmental stages. The results showed that the cotyledons kept growing slowly to maintain the normal physiological activities of seedlings by balancing phytohormone levels, accumulating osmoprotectants and antioxidants, and scavenging reactive oxygen species (ROS). Salt stress activated the expression of osmoprotectant-related genes and enhanced the accumulation of related primary metabolites. Furthermore, differentially expressed transcriptional profiles of the cotyledons were also analyzed by cDNA-AFLP to gain an understanding of cotyledons in response to development and salt stress, and the results revealed a progressive increase in the expression level of development-related genes, which accounted for a majority of the total tested TDFs. Meanwhile, key photosynthetic and important salt stress-related genes also actively responded. All these performances suggest that “big cotyledons” are experiencing a delayed but active developmental process, by which S. aralocaspica may survive the harsh condition of the seedling stage. |
format | Online Article Text |
id | pubmed-9208309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92083092022-06-21 The Developmental Delay of Seedlings With Cotyledons Only Confers Stress Tolerance to Suaeda aralocaspica (Chenopodiaceae) by Unique Performance on Morphology, Physiology, and Gene Expression Cao, Jing Li, Xiaorong Chen, Ling He, Meixiang Lan, Haiyan Front Plant Sci Plant Science Cotyledons play an important role in seedling establishment, although they may just exist for a short time and become senescent upon the emergence of euphylla. So far, the detailed function of cotyledons has not been well understood. Suaeda aralocaspica is an annual halophyte distributed in cold deserts; its cotyledons could exist for a longer time, even last until maturity, and they must exert a unique function in seedling development. Therefore, in this study, we conducted a series of experiments to investigate the morphological and physiological performances of cotyledons under salt stress at different developmental stages. The results showed that the cotyledons kept growing slowly to maintain the normal physiological activities of seedlings by balancing phytohormone levels, accumulating osmoprotectants and antioxidants, and scavenging reactive oxygen species (ROS). Salt stress activated the expression of osmoprotectant-related genes and enhanced the accumulation of related primary metabolites. Furthermore, differentially expressed transcriptional profiles of the cotyledons were also analyzed by cDNA-AFLP to gain an understanding of cotyledons in response to development and salt stress, and the results revealed a progressive increase in the expression level of development-related genes, which accounted for a majority of the total tested TDFs. Meanwhile, key photosynthetic and important salt stress-related genes also actively responded. All these performances suggest that “big cotyledons” are experiencing a delayed but active developmental process, by which S. aralocaspica may survive the harsh condition of the seedling stage. Frontiers Media S.A. 2022-06-06 /pmc/articles/PMC9208309/ /pubmed/35734249 http://dx.doi.org/10.3389/fpls.2022.844430 Text en Copyright © 2022 Cao, Li, Chen, He and Lan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Cao, Jing Li, Xiaorong Chen, Ling He, Meixiang Lan, Haiyan The Developmental Delay of Seedlings With Cotyledons Only Confers Stress Tolerance to Suaeda aralocaspica (Chenopodiaceae) by Unique Performance on Morphology, Physiology, and Gene Expression |
title | The Developmental Delay of Seedlings With Cotyledons Only Confers Stress Tolerance to Suaeda aralocaspica (Chenopodiaceae) by Unique Performance on Morphology, Physiology, and Gene Expression |
title_full | The Developmental Delay of Seedlings With Cotyledons Only Confers Stress Tolerance to Suaeda aralocaspica (Chenopodiaceae) by Unique Performance on Morphology, Physiology, and Gene Expression |
title_fullStr | The Developmental Delay of Seedlings With Cotyledons Only Confers Stress Tolerance to Suaeda aralocaspica (Chenopodiaceae) by Unique Performance on Morphology, Physiology, and Gene Expression |
title_full_unstemmed | The Developmental Delay of Seedlings With Cotyledons Only Confers Stress Tolerance to Suaeda aralocaspica (Chenopodiaceae) by Unique Performance on Morphology, Physiology, and Gene Expression |
title_short | The Developmental Delay of Seedlings With Cotyledons Only Confers Stress Tolerance to Suaeda aralocaspica (Chenopodiaceae) by Unique Performance on Morphology, Physiology, and Gene Expression |
title_sort | developmental delay of seedlings with cotyledons only confers stress tolerance to suaeda aralocaspica (chenopodiaceae) by unique performance on morphology, physiology, and gene expression |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208309/ https://www.ncbi.nlm.nih.gov/pubmed/35734249 http://dx.doi.org/10.3389/fpls.2022.844430 |
work_keys_str_mv | AT caojing thedevelopmentaldelayofseedlingswithcotyledonsonlyconfersstresstolerancetosuaedaaralocaspicachenopodiaceaebyuniqueperformanceonmorphologyphysiologyandgeneexpression AT lixiaorong thedevelopmentaldelayofseedlingswithcotyledonsonlyconfersstresstolerancetosuaedaaralocaspicachenopodiaceaebyuniqueperformanceonmorphologyphysiologyandgeneexpression AT chenling thedevelopmentaldelayofseedlingswithcotyledonsonlyconfersstresstolerancetosuaedaaralocaspicachenopodiaceaebyuniqueperformanceonmorphologyphysiologyandgeneexpression AT hemeixiang thedevelopmentaldelayofseedlingswithcotyledonsonlyconfersstresstolerancetosuaedaaralocaspicachenopodiaceaebyuniqueperformanceonmorphologyphysiologyandgeneexpression AT lanhaiyan thedevelopmentaldelayofseedlingswithcotyledonsonlyconfersstresstolerancetosuaedaaralocaspicachenopodiaceaebyuniqueperformanceonmorphologyphysiologyandgeneexpression AT caojing developmentaldelayofseedlingswithcotyledonsonlyconfersstresstolerancetosuaedaaralocaspicachenopodiaceaebyuniqueperformanceonmorphologyphysiologyandgeneexpression AT lixiaorong developmentaldelayofseedlingswithcotyledonsonlyconfersstresstolerancetosuaedaaralocaspicachenopodiaceaebyuniqueperformanceonmorphologyphysiologyandgeneexpression AT chenling developmentaldelayofseedlingswithcotyledonsonlyconfersstresstolerancetosuaedaaralocaspicachenopodiaceaebyuniqueperformanceonmorphologyphysiologyandgeneexpression AT hemeixiang developmentaldelayofseedlingswithcotyledonsonlyconfersstresstolerancetosuaedaaralocaspicachenopodiaceaebyuniqueperformanceonmorphologyphysiologyandgeneexpression AT lanhaiyan developmentaldelayofseedlingswithcotyledonsonlyconfersstresstolerancetosuaedaaralocaspicachenopodiaceaebyuniqueperformanceonmorphologyphysiologyandgeneexpression |